| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,668,038 | Δ1 bp | 9.5% | intergenic (+56/‑111) | prpF → / → prpD | aconitate isomerase/2‑methylcitrate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,668,038 | 0 | G | . | 9.5% | 121.6 / 7.6 | 42 | intergenic (+56/‑111) | prpF/prpD | aconitate isomerase/2‑methylcitrate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (15/21); new base . (2/2); total (19/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 | |||||||||||
ATGGTGAGTGGACGGTAACCAAGGCAATCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTA > NC_002947/2667891‑2668186 | aTGGTGAGTGGACGGTAACCAAGGCAATCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGc < 2:162899/149‑1 (MQ=255) gTGGACGGTAACCAAGGCAATCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAACTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTcc < 1:177136/149‑1 (MQ=255) gACGGTAACCAAGGCAATCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCcac < 6:267796/149‑1 (MQ=255) aCGGTAACCAAGGCAATCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTc > 8:128454/1‑144 (MQ=255) aCGGTAACCAAGGCAATCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTc < 7:128454/144‑1 (MQ=255) cAAGGCAATCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATca > 3:264303/1‑149 (MQ=255) aaGGCAATCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTTCCCG‑CTCCCACAGGTAATca > 8:165272/1‑148 (MQ=255) aaGGCAATCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATca > 2:220111/1‑148 (MQ=255) aGGCAATCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATca > 7:4932/1‑147 (MQ=255) ggCAATCATGAGCCGTAGTTCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGTGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAg < 1:80557/149‑1 (MQ=255) gaaaTCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAgg < 8:219557/147‑1 (MQ=255) gCAATCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAgg < 2:185125/149‑1 (MQ=255) gCAATCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAgg < 7:239751/149‑1 (MQ=255) gCAATCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAgg > 3:251355/1‑149 (MQ=255) gCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTT‑CCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCt > 1:252267/1‑149 (MQ=255) gCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTT‑CCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCt > 1:248687/1‑149 (MQ=255) ccGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCt < 5:279853/149‑1 (MQ=255) cTGATGGAGGGCTGGGTTCGCGTGCCTGGCTATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTgg < 6:232031/148‑1 (MQ=255) cTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTgg < 8:4932/148‑1 (MQ=255) tGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCCTCCCACAGGTAATCACAGGGTTTGAATGCtg < 1:192548/134‑1 (MQ=255) tGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCtg > 2:192548/1‑133 (MQ=255) ggCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCgg > 5:191233/1‑145 (MQ=255) ggCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCgg < 6:191233/145‑1 (MQ=255) cTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGc > 7:12515/1‑149 (MQ=255) tGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGcc > 1:223621/1‑149 (MQ=255) ccTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTT‑CCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTg < 2:248687/148‑1 (MQ=255) ccTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTT‑CCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTg < 2:252267/148‑1 (MQ=255) cTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCtg > 7:135429/1‑109 (MQ=255) cTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCtg < 8:135429/109‑1 (MQ=255) cTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTggg > 5:163011/1‑124 (MQ=255) cTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTggg < 6:163011/124‑1 (MQ=255) ctaaaGCTTCTAAAGAACCACTGGCGCTCGGCTTCAAGCGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGTTAATCACAGGGTTTGAATGCTGTTATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCAcag < 2:223621/145‑1 (MQ=37) ttCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCtgt < 6:304253/110‑1 (MQ=255) ttCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCtgt > 5:304253/1‑110 (MQ=255) acccaccacTGGGGCTGGGCTTCATGTGGGGCGGCTTTGGGGCCCTTCGCGGGCTTGCCGG‑CTCCCACTGGTAATCACTGGGTTTGAATGCTGTTATATCCCTGTGGGTGGGGGAAAGCCCGCGAAGGGCTGCACAGCAGCCCCgg < 4:264303/143‑1 (MQ=255) gCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCtgtg < 2:189826/93‑1 (MQ=255) gCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCtgtg > 1:189826/1‑93 (MQ=255) gcgcTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCATTCCCG‑CTCCCACACGTTATCACACTGGTTTAAAGCAGGGATTTCCCGGTGGGAGAGGGCAAGCCCGCGAACGGCATCACCGCCGCCCCGGGAATTAGGAAgg > 8:238589/1‑147 (MQ=255) cGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTgg < 7:165272/149‑1 (MQ=255) ggggCCGCTTTGCGGCCCTTCGCGGGCTTGCCCC‑CAACCACAGGTTATAACACGGGTTGAATGCAGTGATATACCAGTGGGCGCGGGCACGCCCGCGACGGGCTGCACAGCAGCCCCGGGAATTATGAAGGGAGCTGGATATTAgcgc > 2:259960/1‑148 (MQ=255) caaagCGGCCCTTCGCGGGCGTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCcgc < 7:171088/85‑1 (MQ=255) cTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCGCAGCAGCCCCGGCAATTCTGAAGGGCGCTGGATATGAGCGCCCACGTAg > 7:281310/1‑149 (MQ=255) gCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTa > 5:59223/1‑149 (MQ=255) | ATGGTGAGTGGACGGTAACCAAGGCAATCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCG‑CTCCCACAGGTAATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTA > NC_002947/2667891‑2668186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |