Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,159,357 Δ1 bp 9.5% coding (1376/2097 nt) opuA → glycine betaine ABC transporter ATP‑binding protein/permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,159,3570A. 9.5% 127.6 / 7.7 42coding (1376/2097 nt)opuAglycine betaine ABC transporter ATP‑binding protein/permease
Reads supporting (aligned to +/- strand):  ref base A (20/18);  new base . (2/2);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

GGACATTGGTGCAGTTTCCCTCCGATCGCGACAGTATGCTGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGTTAGCCCTGGGGTATATCGGCCTGTTCGGTTTCTGGGAGCGTACGATTTCC  >  NC_002947/3159210‑3159505
                                                                                                                                                   |                                                                                                                                                    
ggACATTGGTGCAGTTTCCCTCCGATCGCGACAGTATGCTGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGaa                                                                                                                                                     <  1:126961/149‑1 (MQ=255)
ggACATTGGTGCAGTTTCCCTCCGATCGCGACAGTATGCTGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGaa                                                                                                                                                     <  1:131653/149‑1 (MQ=255)
        gTGCAGTTTCCCTCCGATCGCGACAGTATGCTGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCgct                                                                                                                                             >  4:132191/1‑149 (MQ=255)
        gTGCAGTTTCCCTCCGATCGCGACAGTATGCTGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCAGTGCGCACCGTCATCAATGGCGTCGAAGGTGCgct                                                                                                                                             >  7:32049/1‑149 (MQ=255)
                  ccTCCGATCGCGACAGTATGCTGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCgctgggct                                                                                                                                        <  1:155497/144‑1 (MQ=255)
                  ccTCCGATCGCGACAGTATGCTGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCgctgggct                                                                                                                                        >  2:155497/1‑144 (MQ=255)
                   cTCCGATCGCGACAGTATGCTGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGa                                                                                                                                                      >  8:18040/1‑129 (MQ=255)
                      cGATCGCGACAGTATGCTGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGt                                                                                                                               <  5:269636/149‑1 (MQ=255)
                           gcgACAGTATGCTGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGcc                                                                                                                           <  5:222549/148‑1 (MQ=255)
                           gcgACAGTATGCTGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCg                                                                                                                          >  7:90859/1‑149 (MQ=255)
                            cgACAGTATGCTGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCAgg                                                                                                                         >  7:38005/1‑149 (MQ=255)
                                  tATGCTGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCgg                                                                                                                   >  6:117994/1‑149 (MQ=255)
                                       tGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCAACAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCtt                                                                                                              >  4:97757/1‑149 (MQ=255)
                                                      ccATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTAcctg                                                                                               >  3:3365/1‑149 (MQ=255)
                                                           gACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTAcctggcctg                                                                                          <  4:3365/149‑1 (MQ=255)
                                                             cgcgTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACctggcctggc                                                                                        <  8:90859/149‑1 (MQ=255)
                                                                 ttGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTAcctg                                                                                               >  1:51807/1‑138 (MQ=255)
                                                                 ttGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTAcctg                                                                                               <  2:51807/138‑1 (MQ=255)
                                                                   gATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTcc                                                                                   <  1:103583/148‑1 (MQ=255)
                                                                             tctcAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGgcg                                                                            <  4:89593/145‑1 (MQ=255)
                                                                             tctcAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGgcg                                                                            >  3:89593/1‑145 (MQ=255)
                                                                               tcAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCG‑AGGTGCgctgggctgg                                                                                                                                      <  6:118340/84‑1 (MQ=255)
                                                                               tcAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCG‑AGGTGCgctgggctgg                                                                                                                                      <  6:113601/84‑1 (MQ=255)
                                                                               tcAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCG‑AGGTGCgctgggctgg                                                                                                                                      >  5:113601/1‑84 (MQ=255)
                                                                               tcAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCG‑AGGTGCgctgggctgg                                                                                                                                      >  5:118340/1‑84 (MQ=255)
                                                                                       ccTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCt                                                               >  3:298713/1‑148 (MQ=255)
                                                                                        cTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTga                                                             >  5:54870/1‑149 (MQ=255)
                                                                                        cTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTga                                                             >  4:72578/1‑149 (MQ=255)
                                                                                        cTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATCCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTga                                                             >  3:257480/1‑149 (MQ=255)
                                                                                         ttCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTgac                                                            >  2:124211/1‑149 (MQ=255)
                                                                                         ttCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTgac                                                            >  5:87430/1‑149 (MQ=255)
                                                                                           cGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTgac                                                            <  6:47959/147‑1 (MQ=255)
                                                                                                 cgcATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGt                                                    <  8:38005/149‑1 (MQ=255)
                                                                                                           ggggTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGTTAGCCCTggg                                          <  6:87430/149‑1 (MQ=255)
                                                                                                                    gTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGTTAGCCCTGGGGTATATCg                                  <  6:54870/148‑1 (MQ=255)
                                                                                                                     tGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGTTAGCCCTGGGGTATATCGGc                                >  5:98803/1‑149 (MQ=255)
                                                                                                                         ggTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGTTAGCCCTGGGGTATATCGGCCTGt                            >  6:264561/1‑149 (MQ=255)
                                                                                                                                  cGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGTTAGCCCTGGGGTATATCGGCCTGTTCGGTTTCt                   >  6:261744/1‑149 (MQ=255)
                                                                                                                                    tcatcaATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGTTAGCCCTGGGGTATATCGGCCTGTTCGGTTTCTgg                 <  5:117994/149‑1 (MQ=255)
                                                                                                                                    tcatcaATGGCGTCGAAGGTGCGCTGGGCTGGATCCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGTTAGCCCTGGGGTATATCGGCCTGTTCGGTTTCTgg                 <  4:257480/149‑1 (MQ=255)
                                                                                                                                     catcaATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCTTGACGACTGCGTTAGCCCTGGTGTATATCGGCCTGTTCGGTTTCTgg                 <  4:298713/148‑1 (MQ=255)
                                                                                                                                      atcaATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGTTAGCCCTGGGGTATATCGGCCTGTTCGGTTTCTGGGa               <  3:259470/149‑1 (MQ=255)
                                                                                                                                              cGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGTTAGCCCTGGGGTATATCGGCCTGTTCgg                        >  7:68591/1‑132 (MQ=255)
                                                                                                                                              cGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGTTAGCCCTGGGGTATATCGGCCTGTTCgg                        <  8:68591/132‑1 (MQ=255)
                                                                                                                                                  gAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGTTAGCCCTGGGGTATATCGGCCTGTTCGGTTTCTGGGAGCGTACGATTTc   <  1:124211/149‑1 (MQ=255)
                                                                                                                                                   aaGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGTTAGCCCTGGGGTATATCGGCCTGTTCGGTTTCTGGGAGCGTACGATTTcc  <  3:97757/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGACATTGGTGCAGTTTCCCTCCGATCGCGACAGTATGCTGGCGGCCGCTGGTGCCATCGACGCGTTGATTGGCTGGTCTCAGGTAACCTTCGAAAGCGCATTCGTCGGGGTGATCGTGGCGGTGCGCACCGTCATCAATGGCGTCGAAGGTGCGCTGGGCTGGATTCCGTGGCCGGTACCGGCGCTTGCATTGGTCTACCTGGCCTGGCGCTCCGCCGGCGCGATCCTGGCCCTGACGACTGCGTTAGCCCTGGGGTATATCGGCCTGTTCGGTTTCTGGGAGCGTACGATTTCC  >  NC_002947/3159210‑3159505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑