Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,962,576	Δ1 bp	7.5%	coding (774/1152 nt)	PP_3492 →	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,962,576	0	A	.	7.5%	162.9 / 7.7	53	coding (774/1152 nt)	PP_3492	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (26/23);  new base . (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGCG  >  NC_002947/3962438‑3962722                                                                                                                                            |                                                                                                                                                    ggTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑gcgg                                                                                                                                          >  3:149112/1‑149 (MQ=255)  gTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑gcggc                                                                                                                                         >  4:68283/1‑149 (MQ=255)   tCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑gcggcg                                                                                                                                        >  2:7456/1‑149 (MQ=255)       cTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCAGCG‑CCGC‑TTCGAATGCGGCGCggg                                                                                                                                    >  7:108499/1‑149 (MQ=39)          cGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGt                                                                                                                                   <  6:223739/147‑1 (MQ=255)          cGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCAGCG‑CCGC‑TTCGAATGCGGCGCgggcg                                                                                                                                  <  8:108499/148‑3 (MQ=39)            aCAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTAc                                                                                                                               >  7:183819/1‑149 (MQ=255)              aaCTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCa                                                                                                                                                     <  5:280177/126‑1 (MQ=255)              aaCTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCa                                                                                                                                                     >  6:280177/1‑126 (MQ=255)               aCTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCCGCATTCGAA‑GCGGCGCTGGTCTACTc                                                                                                                             <  1:17878/149‑1 (MQ=255)                cTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTcgc                                                                                                                           <  1:7456/149‑1 (MQ=255)                           cTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCAt                                                                                                                 >  4:14293/1‑148 (MQ=255)                              ccgccAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAg                                                                                                             >  2:105190/1‑149 (MQ=255)                                  cAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTg                                                                                                         <  8:183819/149‑1 (MQ=255)                                     tatCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGc                                                                                                       >  4:19438/1‑148 (MQ=255)                                          tCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCCACCTCGAAGGCGCCCGTATCGGCATTGCCGCCCCGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACACGCGTGCCCGCATCCAGTTTGGCACGcc                                                                                                  >  7:27369/1‑148 (MQ=255)                                             gTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATc                                                                                              <  3:68283/149‑1 (MQ=255)                                                aGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAAc                                                                                           <  3:14293/149‑1 (MQ=255)                                                aGCGTGGCAAGGGCCTGGACATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAAc                                                                                           <  1:219746/149‑1 (MQ=255)                                                    tGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAac                                                                                       <  4:149112/149‑1 (MQ=255)                                                       cAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAacac                                                                                     >  1:254932/1‑148 (MQ=255)                                                         aGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAacactgc                                                                                  >  1:146398/1‑146 (MQ=255)                                                          gggCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCaga                                                                                 <  1:187746/149‑1 (MQ=255)                                                             ccTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCa                                                                              >  1:209654/1‑149 (MQ=255)                                                              cTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCAt                                                                             >  5:187124/1‑149 (MQ=255)                                                                ggCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTg                                                                           >  5:313404/1‑149 (MQ=255)                                                                 gCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCAtt                                                                            <  8:122218/147‑1 (MQ=255)                                                                        cccTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACctg                                                                   >  6:51306/1‑149 (MQ=255)                                                                            gTCCAACCTCGAAGGCGGTCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTgg                                                               >  1:92158/1‑149 (MQ=255)                                                                             tCCAACCTCGAAGGCGGTCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGc                                                              <  2:92158/149‑1 (MQ=255)                                                                             tCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTgg                                                               <  6:313404/148‑1 (MQ=255)                                                                             tCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTAGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGATCATTGCCCACCGGCTGGc                                                              >  6:17922/1‑149 (MQ=255)                                                                                 aCCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGa                                                           >  2:75780/1‑148 (MQ=255)                                                                                    tCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAtg                                                       >  1:122101/1‑149 (MQ=255)                                                                                      gAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAtgc                                                      <  1:105190/148‑1 (MQ=255)                                                                                            ggcCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAtgcaggtgca                                               <  6:187124/149‑1 (MQ=255)                                                                                             gcCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAtgcaggtgcag                                              >  4:139643/1‑149 (MQ=255)                                                                                               cGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATgcaggtgcaggt                                            <  3:19438/149‑1 (MQ=255)                                                                                               cGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATgcaggtgcaggt                                            <  2:209654/149‑1 (MQ=255)                                                                                                      gCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAAcgcc                                     >  6:297422/1‑149 (MQ=255)                                                                                                       cATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAAcgccg                                    >  7:154186/1‑149 (MQ=255)                                                                                                            ccgccCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGt                               >  8:186448/1‑149 (MQ=255)                                                                                                               cccAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTg                            >  3:75845/1‑149 (MQ=255)                                                                                                                cccggCCGTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGGCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGc                           <  8:27369/146‑1 (MQ=255)                                                                                                                  aGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGttt                             >  5:43229/1‑145 (MQ=255)                                                                                                                  aGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGttt                             <  6:43229/145‑1 (MQ=255)                                                                                                                      cTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAt                                                        >  5:251951/1‑114 (MQ=255)                                                                                                                      cTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAt                                                        <  6:251951/114‑1 (MQ=255)                                                                                                                      cTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATcc                     <  2:254932/149‑1 (MQ=255)                                                                                                                         gggCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGc                           >  8:84416/1‑140 (MQ=255)                                                                                                                         gggCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGc                           <  7:84416/140‑1 (MQ=255)                                                                                                                                accagcg‑cCGC‑TTCGAATGCGGCGCGGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAtgcaggtgcagg                                             <  3:13895/111‑1 (MQ=255)                                                                                                                                accagcg‑cCGC‑TTCGAATGCGGCGCGGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAtgcaggtgcagg                                             >  4:13895/5‑115 (MQ=255)                                                                                                                                    gcg‑cCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGccgcccgc       <  5:297422/149‑1 (MQ=255)                                                                                                                                          gcATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTgcg  <  3:139643/149‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                    GGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCG‑CCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGCG  >  NC_002947/3962438‑3962722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑