Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,479,939:1	+G	9.8%	coding (463/993 nt)	prmA →	ribosomal protein L11 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,479,939	1	.	G	9.8%	184.1 / 13.0	51	coding (463/993 nt)	prmA	ribosomal protein L11 methyltransferase
Reads supporting (aligned to +/- strand):  ref base . (24/22);  new base G (2/3);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAGGTGTTCGCCCACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCACCCAGGTGCTGGACTTTGG  >  NC_002947/5479791‑5480084                                                                                                                                                       |                                                                                                                                                  aCAGGTGTTCGCCCACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCt                                                                                                                                                    >  1:146765/1‑149 (MQ=255) aCAGGTGTTCGCCCACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCAGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCt                                                                                                                                                    <  6:23048/149‑1 (MQ=255)          cGCCCACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTG‑CCAGCTGGGCACGAt                                                                                                                                           <  1:89215/148‑1 (MQ=255)          cGCCCACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTG‑CCAGCTGGGCACGAt                                                                                                                                           <  3:245906/148‑1 (MQ=255)           gCCCACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGc                                                                                                                                         <  6:143178/149‑1 (MQ=255)           gCCCACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTG‑CCAGCTGGGCACGAtc                                                                                                                                          >  8:268222/1‑147 (MQ=255)            cccACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGcc                                                                                                                                        >  7:131422/1‑149 (MQ=255)             ccACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGccc                                                                                                                                       >  7:168668/1‑149 (MQ=255)              cACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTG‑CCAGCTGGGCACGAtccaca                                                                                                                                      <  7:268222/149‑6 (MQ=255)               aCCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCg                                                                                                                                     >  1:293657/1‑149 (MQ=255)                    cgcCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGaaaa                                                                                                                                <  3:25986/149‑1 (MQ=255)                     gcCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAg                                                                                                                               <  7:139958/149‑1 (MQ=255)                       ctgctgACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAATGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGa                                                                                                                             >  6:5286/1‑149 (MQ=255)                       ctgctgACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGa                                                                                                                             >  6:1811/1‑149 (MQ=255)                          ctgACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGAc                                                                                                                            <  3:117667/147‑1 (MQ=255)                             aCCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGt                                                                                                                       <  6:6837/149‑1 (MQ=255)                              ccGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTg                                                                                                                      <  5:1811/149‑1 (MQ=255)                               cGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTg                                                                                                                      >  6:142494/1‑148 (MQ=255)                               cGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTg                                                                                                                      <  5:142494/148‑1 (MQ=255)                                 gcgcCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAAc                                                                                                                   >  6:143541/1‑149 (MQ=255)                                           gCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCg                                                                                                         >  8:71258/1‑149 (MQ=255)                                            cccGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCg                                                                                                         <  2:293657/148‑1 (MQ=255)                                             ccGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGATCAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GCCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGa                                                                                                        >  1:84358/1‑149 (MQ=255)                                                   aCCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑gg                                                                                                                                                 <  1:25161/101‑1 (MQ=255)                                                   aCCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑gg                                                                                                                                                 >  2:25161/1‑101 (MQ=255)                                                      aGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGggcctg                                                                                              <  2:146765/149‑1 (MQ=255)                                                            aGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCttt                                                                                        <  8:168668/149‑1 (MQ=255)                                                                gATCGAGGACCAGGACTGGGAACGCAGCTGNATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCa                                                                                    >  1:28393/1‑149 (MQ=255)                                                                  tCGAGGCCCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCAcc                                                                                  >  8:256004/1‑149 (MQ=255)                                                                      ggACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCa                                                                              <  5:143541/149‑1 (MQ=255)                                                                          cAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGggc                                                                                                 <  2:89817/126‑1 (MQ=255)                                                                          cAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGggc                                                                                                 >  1:89817/1‑126 (MQ=255)                                                                             gACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCAccc                                                                       >  5:207388/1‑149 (MQ=255)                                                                             gACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCAccc                                                                       >  8:253310/1‑149 (MQ=255)                                                                                tGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCccac                                                                    >  8:79723/1‑149 (MQ=255)                                                                                         aGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCt                                                           <  7:253310/149‑1 (MQ=255)                                                                                                  ga‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTgg                                                   <  2:36309/148‑1 (MQ=255)                                                                                                   a‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGaa                                                 >  1:5992/1‑149 (MQ=255)                                                                                                       aCTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATgg                                              <  8:131422/149‑1 (MQ=255)                                                                                                         ttCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCt                                            >  1:92613/1‑149 (MQ=255)                                                                                                               ccGATGCGCTTCGGCCGCCGCCTGAGGATCGTGCCAAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGCCCCGCGCCTGGCCGTTGGCCCCGGCACTCACCCCACCACGGCCCTCTGCCTGGAATGGCTCGACgg                                      >  8:77236/1‑149 (MQ=255)                                                                                                                      gcTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCcagcag                               >  6:67491/1‑149 (MQ=255)                                                                                                                       cTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCagcagc                              <  3:291498/149‑1 (MQ=255)                                                                                                                       cTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCagcagc                              <  6:207388/149‑1 (MQ=255)                                                                                                                               gccgccTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGc                      <  7:71258/149‑1 (MQ=255)                                                                                                                                 cgccTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCAc                    <  2:84358/149‑1 (MQ=255)                                                                                                                                       tgGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCACCCAGGt              <  2:92613/149‑1 (MQ=255)                                                                                                                                        gcaTCGTG‑CCAGCTGGGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCACCCAGGTg             >  1:21273/3‑149 (MQ=255)                                                                                                                                             gTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCAcc                                                                        >  2:60388/1‑85 (MQ=255)                                                                                                                                             gTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCAcc                                                                        <  1:60388/85‑1 (MQ=255)                                                                                                                                             gTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCACCCAGGTggggga        >  6:173497/1‑144 (MQ=255)                                                                                                                                              tGCCCAGCT‑GGCGCGATGCCCCGGAAAAGGACGCCGTGAACCTGGTGCTCGACCCGGGCCTGGCCTGTGGCACCGGCAGGCAGCCCACCACGGCCCTGTGGGTGGAGTGGCTCGACGGGCAGCAGCGTGAAGGCGCCGGGGTGCTGGag       >  4:37810/1‑148 (MQ=255)                                                                                                                                               gCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCACCCAGGTGCTGGACt      >  7:171605/1‑149 (MQ=255)                                                                                                                                               gCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGACAGCAGCTTGAAGGCACCCAGGTGCTGGACt      >  7:167040/1‑149 (MQ=255)                                                                                                                                                   aGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCACCCAGGTGCTGGACTTTgg  <  7:256004/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                  ACAGGTGTTCGCCCACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGA‑CAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCACCCAGGTGCTGGACTTTGG  >  NC_002947/5479791‑5480084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑