Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,997,189:1	+G	7.4%	intergenic (‑158/+42)	PP_5252 ← / ← PP_5253	hypothetical protein/arylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,997,189	1	.	G	7.4%	200.2 / 11.2	54	intergenic (‑158/+42)	PP_5252/PP_5253	hypothetical protein/arylesterase
Reads supporting (aligned to +/- strand):  ref base . (25/25);  new base G (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

GACTAGGGGCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACGAAT  >  NC_002947/5997042‑5997335                                                                                                                                                     |                                                                                                                                                   gACTAGGGGCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACa‑a                                                                                                                                                   >  8:12554/1‑149 (MQ=255)      ggggCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCt                                                                                                                                              >  8:2673/1‑149 (MQ=255)      ggggCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCt                                                                                                                                              >  3:78634/1‑149 (MQ=255)        ggCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTg                                                                                                                                             >  2:21865/1‑148 (MQ=255)        ggCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTg                                                                                                                                             >  6:227273/1‑148 (MQ=255)        ggCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTg                                                                                                                                             >  8:262206/1‑148 (MQ=255)         gCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTa                                                                                                                                           <  7:2673/149‑1 (MQ=255)          ccGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAg                                                                                                                                          <  5:57358/149‑1 (MQ=255)          ccGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAg                                                                                                                                          <  7:262206/149‑1 (MQ=255)          ccGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAg                                                                                                                                          <  6:251028/149‑1 (MQ=255)                tggggACTGCGAAGCATGCCGGTGGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGGTGAAGAAGGGTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCTCTCCTACA‑ACGGCTGTAGGAGCgg                                                                                                                                    <  1:50/148‑1 (MQ=39)                gggggACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGACGT‑TGTAGGAGCgg                                                                                                                                    <  4:234760/149‑1 (MQ=255)                      cTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTAc                                                                                                                              <  5:290435/149‑1 (MQ=255)                       tGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTAcc                                                                                                                             >  7:157932/1‑149 (MQ=255)                       tGCGAAGCATACCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCGACA‑ACGTCTGTAGGAGCGGGTTTAcc                                                                                                                             >  1:257554/1‑149 (MQ=255)                         cGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGACGT‑TGTAGGAGc                                                                                                                                      >  8:146320/1‑138 (MQ=255)                         cGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGACGT‑TGTAGGAGc                                                                                                                                      <  7:146320/138‑1 (MQ=255)                              cATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAAt                                                                                                                      <  2:42476/149‑1 (MQ=255)                                  ccGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGttt                                                                                                                  <  1:198707/149‑1 (MQ=255)                                   cGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTc                                                                                                                 <  7:12554/149‑1 (MQ=255)                                             aaGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTAc                                                                                                       <  8:237870/149‑1 (MQ=255)                                                gCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGc                                                                                                    <  1:21865/149‑1 (MQ=255)                                                     ggCTTGAATGTAGGTGCGGTGGGTGGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGa                                                                                                 <  4:254631/147‑1 (MQ=255)                                                          gAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACg                                                                                          >  1:181009/1‑149 (MQ=255)                                                          gAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGAGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACg                                                                                          >  1:212847/1‑149 (MQ=255)                                                           aaTGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCCCTCCTACC‑AAGTCTGTAGGGGCGGGTTTACCCGCGAATGTTTGCCACAATTACCGCTGAAGGGACGc                                                                                         >  4:262216/1‑149 (MQ=38)                                                                    gCGGTCGGTTGAAGAAGGTTAGCCCTGCTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTTAACCCGCATCGACA‑ACGTAGTTAGGATCGGGTTTTCACGCGAATGTTTCCCACTATTACCGCTGAAGGAACGCCAGCAAGt                                                                                 >  6:95355/1‑148 (MQ=255)                                                                      ggTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCt                                                                              <  5:120043/149‑1 (MQ=255)                                                                      ggTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCt                                                                              <  4:78634/149‑1 (MQ=255)                                                                        tGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCa                                                                            <  5:227273/149‑1 (MQ=255)                                                                                    ggTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCTGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCtgctgc                                                                <  6:308025/149‑1 (MQ=255)                                                                                     gtaaGCCCTGGACAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGTAAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGTTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCtgctgc                                                                <  5:52346/145‑1 (MQ=255)                                                                                     gTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTgcg                                                               <  1:175218/149‑1 (MQ=255)                                                                                         gCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCAt                                                            <  2:212847/148‑1 (MQ=255)                                                                                           ccTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCCCTCCTACA‑ACGTCAGTAGGACCGGGGTTACCCGCGAATGTTTCCCACAATTACCGCAGAACGAACGCCAGCAAGACCTCATTAAGCTGCTGCGCATGAg                                                         >  6:63869/1‑149 (MQ=38)                                                                                            cTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGTGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGt                                                        <  4:112795/149‑1 (MQ=255)                                                                                            cTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTgcgc                                                              >  1:171502/1‑143 (MQ=255)                                                                                            cTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTgcgc                                                              <  2:171502/143‑1 (MQ=255)                                                                                                 cAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAAACCGCTCCTACA‑ACGTGTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCCCAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTg                                                   >  2:95594/1‑149 (MQ=255)                                                                                                                cTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGTAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTgcggcg                                    <  8:157932/149‑1 (MQ=255)                                                                                                                 tGGGCCGGCCTCTTCGCGGGTAAACCCGCTACTAAC‑ACGTCTGTAAGAGCGGGTTTACCCCCGAATTTTTACACCCAATACCCCTGCACGAACCCCCGCAACTCCTCATTCACCTGCTTCGCATGATTCCATGCAAAACCGTGcgccgc                                   >  2:223415/1‑149 (MQ=16)                                                                                                                    gccggccTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGcccc                                >  6:139753/1‑149 (MQ=255)                                                                                                                     ccggccTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCg                               >  6:55388/1‑149 (MQ=255)                                                                                                                             ttCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATacac                       <  2:181009/149‑1 (MQ=255)                                                                                                                                      aaaCCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTc              >  6:89065/1‑149 (MQ=255)                                                                                                                                      aaaCCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTc              >  2:289128/1‑149 (MQ=255)                                                                                                                                       aaCCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTAGCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGTCCCGGCATACACCTTCAGCTc              <  5:63869/148‑1 (MQ=255)                                                                                                                                         cccGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGgc           >  1:275498/1‑149 (MQ=255)                                                                                                                                          ccGCTCCTACAGACGT‑TGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGgcg          >  6:303210/1‑149 (MQ=255)                                                                                                                                                cTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACg     >  4:104125/1‑148 (MQ=255)                                                                                                                                                 tACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCtt                    <  6:88560/132‑1 (MQ=255)                                                                                                                                                 tACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCtt                    >  5:88560/1‑132 (MQ=255)                                                                                                                                                 tACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACGaa   >  2:208719/1‑149 (MQ=255)                                                                                                                                                 tACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACGa    >  2:142573/1‑148 (MQ=255)                                                                                                                                                   cA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACGAAt  >  3:171214/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GACTAGGGGCCGGCAGGGGGACTGCGAAGCATGCCGGTTGATGCAAGGCCTGGGCTTGAATGGATGTGCGGTGGGTTGAAGAAGGTTAGCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑ACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACGAAT  >  NC_002947/5997042‑5997335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑