| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 278,742:1 | +A | 100% | intergenic (‑237/+77) | PP_0224 ← / ← sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 278,742 | 1 | . | A | 86.5% | 104.2 / 11.3 | 37 | intergenic (‑237/+77) | PP_0224/sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (1/4); new base A (15/17); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑A‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCG > NC_002947/278595‑278883 | aTGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑t > 3:126126/1‑148 (MQ=255) cAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGc < 1:52514/147‑1 (MQ=255) gCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTcc > 5:118616/1‑148 (MQ=255) tGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTa > 5:110087/1‑148 (MQ=255) tCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTACAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTacac > 5:200648/1‑148 (MQ=255) tcAGAGGTGTGCTGGTGCGGTTGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑GCCGCTCCTACACACATGc < 8:4566/148‑1 (MQ=255) gACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTc < 8:216431/149‑1 (MQ=255) cGTGTGATGGTCCTGTGGTCAGTTATATATACCCTAATGATTTATAAAATTTTTATTAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTAAACACATGCCTCaa < 2:72912/149‑1 (MQ=255) gtgtGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAg < 4:126126/149‑1 (MQ=255) gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCtgtg > 6:293010/1‑148 (MQ=255) tggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa < 6:31856/149‑1 (MQ=255) cggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGc > 4:118630/1‑149 (MQ=255) tatataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGtt < 5:293010/149‑1 (MQ=255) taCACTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTACAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTAc < 6:200648/148‑1 (MQ=255) ccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCcgc < 8:38439/149‑1 (MQ=255) aTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCtgtg > 4:101704/1‑121 (MQ=255) aTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGaaa < 6:118616/149‑1 (MQ=255) tGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑ACCGCTCATACACAAATTCCTAAAAATAAGTGGACTGTATGCTCGGGTTTAACCCCGaaat > 3:166040/1‑148 (MQ=255) aTGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCggg < 5:25963/118‑1 (MQ=255) aTGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCggg > 6:25963/1‑118 (MQ=255) ttactaaatTTTTGTATTTTTTATACAAGGGGGATTTGTTACTGAGGGGCGGGGGTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCATGTTCTGTCCACTGTTGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACt < 6:110087/146‑1 (MQ=255) ttttaactTTTTGTAATATTTATAGAAGGGGTATTTGTTACAGCGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTTGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTc < 8:145587/145‑1 (MQ=255) aactaactTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑A‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCgg < 1:39165/149‑1 (MQ=255) aactaactTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑A‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCg > 2:39165/1‑148 (MQ=255) actaactTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCGACACACATGCCTCAAGCTCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCgg > 7:266451/1‑149 (MQ=255) aactTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCgagat > 7:145587/1‑145 (MQ=255) tttGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCg > 3:186457/1‑147 (MQ=255) tatTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTGCTCGGAGGCCGCAGTccc > 3:190371/1‑147 (MQ=255) tttatggggtATTTGTTAGTGTGTGGCGGGGCTCTTCGCGGG‑T‑A‑ACCCCGTTCCTACACACTTGCGTCAAGATGTGTGCATTGTCGGTGCGGTTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAAc < 4:166040/143‑1 (MQ=255) tggggtATTTGTTACCTAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACTTGCCTCAAGATCTGTGCACTGTTGGAGGGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTgc < 8:266451/147‑1 (MQ=255) ggctaTTTGTTACAGCGGTGCCGGGATCTTCGCGTG‑T‑ACA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTTTAGGAGGGGTTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTgcgc < 8:210753/144‑1 (MQ=255) ttGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACt > 4:206896/1‑149 (MQ=255) ttACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGc < 3:118630/148‑1 (MQ=255) tACAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGcc < 3:206896/148‑1 (MQ=255) cAGAGGTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGttt > 2:4205/1‑78 (MQ=255) gTGCCGGGCTCTTCGCGGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGGTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGc > 1:12944/1‑149 (MQ=255) ctTCGCGGGAA‑A‑A‑CCCGCTCCTACACACATCCCTCACGATCTGTGCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCg < 3:88253/149‑1 (MQ=39) gcgGG‑TAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTGCTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGgcg < 4:190371/148‑1 (MQ=255) | ATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑A‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCG > NC_002947/278595‑278883 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |