| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,447,838:1 | +T | 11.8% | intergenic (‑274/‑48) | nicC ← / → nicX | 6‑hydroxynicotinate 3‑monooxygenase/2,5‑dihydroxypyridine 5,6‑dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,447,838 | 1 | . | T | 11.8% | 172.7 / 18.6 | 51 | intergenic (‑274/‑48) | nicC/nicX | 6‑hydroxynicotinate 3‑monooxygenase/2,5‑dihydroxypyridine 5,6‑dioxygenase |
| Reads supporting (aligned to +/- strand): ref base . (21/24); new base T (3/3); total (24/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01 | |||||||||||
ACGAAATCCGGTTGATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGCCAC > NC_002947/4447690‑4447984 | acgaaaTCCGGTTGATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCa < 6:236805/149‑1 (MQ=255) aTCCGGTTGATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACAGAGATGCAGCACCA‑GGCAc > 3:290597/1‑149 (MQ=255) ttACCGTAAATACTGGTCAATATGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCATGGCAGTGCC‑TGGTgct < 1:136805/148‑2 (MQ=255) cGTAAATACTTGTCAATGTGTGGATAGGTAAAGTTTTAACAGATTACTTGCGCTCTCTGGCAGGATGCGGTTTCATCTGCACAATTATGGTGTACAAACTATTATT‑TTGCACCGAGATTCAGCACCA‑GGCACTGCCATGGTGCGCCCCt < 2:133086/149‑1 (MQ=255) cGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCt > 6:150459/1‑149 (MQ=255) gTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTg > 7:246665/1‑149 (MQ=255) tAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGt < 6:59030/149‑1 (MQ=255) aaTACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCa < 4:248475/149‑1 (MQ=255) cTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGc > 2:47480/1‑149 (MQ=255) tGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCATGGCAGTGCC‑TGGTgct > 1:232979/1‑134 (MQ=255) tGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCATGGCAGTGCC‑TGGTgct < 2:232979/135‑2 (MQ=255) gTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCAc > 8:131092/1‑149 (MQ=255) aaTGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCAcc > 2:78935/1‑147 (MQ=255) aTGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAg < 5:65919/149‑1 (MQ=255) tgGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGaa > 4:84856/1‑149 (MQ=255) gATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAAc < 3:84856/148‑1 (MQ=255) gTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACAA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGAt > 3:109199/1‑149 (MQ=255) cGTTTGAACAGTTGTCTTGCGCTCGCTGGGAGGTTGCGTTTTCATCTGCACATGTAGGGTGGACACACTATTATT‑TTGCAGCGAGATGGAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCATCGCCACCCAGGAGAACCCCGATGcgg < 4:109199/149‑3 (MQ=255) gAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAg > 2:296748/1‑149 (MQ=255) aaCAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCAGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAg < 7:242688/148‑1 (MQ=255) gATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATg < 1:78935/149‑1 (MQ=255) ttGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTAATGCACCGAGATGAAGCACAA‑GGCCATGCAATGGTGCCCCCCTGGAAACGCCCCCCAGGAAAACCCCGATGCCGGTGGGCATTGcaca > 1:87996/1‑148 (MQ=255) gcgcTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACt < 2:274143/148‑1 (MQ=255) gctGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTTTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCa < 5:290619/148‑1 (MQ=255) gctGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAg < 5:150459/149‑1 (MQ=255) aGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGAcc > 4:11900/1‑140 (MQ=255) aGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGAcc < 3:11900/140‑1 (MQ=255) tGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGc < 2:87996/149‑1 (MQ=255) cGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCAc < 1:296748/149‑1 (MQ=255) gTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACg < 8:246665/149‑1 (MQ=255) ttCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTa < 4:290597/148‑1 (MQ=255) tCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTAc < 1:47480/148‑1 (MQ=255) aaGTAGGGTGTACACACTATTATT‑TTGCACCGAGCTGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGtc > 5:18789/1‑148 (MQ=255) gTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGc > 3:8877/1‑130 (MQ=255) gTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGc < 4:8877/130‑1 (MQ=255) gTAGGGTGTAAACACTATTATA‑TTGCACCGAGATGCAGCTCCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAATCTGtctgg < 6:226542/149‑3 (MQ=255) ggTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGt < 4:69336/148‑1 (MQ=255) gtgtACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCg > 7:187447/1‑91 (MQ=255) gtgtACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCg < 8:187447/91‑1 (MQ=255) acacTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGaaa > 1:283024/1‑149 (MQ=255) attatt‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACg > 8:202244/1‑146 (MQ=255) aCCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTg > 7:238429/1‑127 (MQ=255) aCCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTg < 8:238429/127‑1 (MQ=255) ccGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGt < 7:131092/149‑1 (MQ=255) gcagcaCCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGTTc > 4:49890/1‑146 (MQ=255) gcagcaCCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTc > 6:75050/1‑146 (MQ=255) cagcaCCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCaa > 2:156837/1‑147 (MQ=255) cgcaCCATGGCAGTGCC‑TGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCaa > 7:274012/2‑146 (MQ=255) cgcaCCATGGCAGTGCC‑TGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAaga < 8:274012/147‑1 (MQ=255) gcaCCATGGCAGTGCC‑TGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGc > 8:35523/1‑149 (MQ=255) aCCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGCCa < 7:202244/149‑1 (MQ=255) cA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGCCac > 4:264873/1‑148 (MQ=255) | ACGAAATCCGGTTGATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATT‑TTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGCCAC > NC_002947/4447690‑4447984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |