Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_002947	1,450,884	Δ5 bp	5.2%	coding (68‑72/906 nt)	PP_1270 →	LysR family transcriptional regulator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	1450889 =	78 (1.590)	4 (0.090)	4/242	NT	5.2%	coding (73/906 nt)	PP_1270	LysR family transcriptional regulator
	?	NC_002947	= 1450883	75 (1.660)					coding (67/906 nt)	PP_1270	LysR family transcriptional regulator

CTCCACCAGGCCATGCACCTGCTCGCGCAGGGCCAACGCACGGGGCGTAGGCACCAGGCCACGGCCGGCGCGCACCAGGATCGGGTCACCCAAGGCATCGCGGATACGCCCCAATGTCCGGCTCATGGCCGCCGGGCTCAGGTTCATGCGCTGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_002947/1451044‑1450889 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgcgctgcgcCCACCACGCTGCCCTCGTCGAGCAAGGCATCGAGGGCGACGAGCAGGTTCATGTCCGGGAGTTGCATGGCCGTTTTCCGTTACAGCTGCGGGAAACGCGATGGTAGCAGGTTGGTGGATTGCATCAGACGCAATGCGCTCTTGCGT  <  NC_002947/1450883‑1450738                                                                                                                                                                                                                                                                                                                CTCCACCAGGCCATGCACCTGCTCGCGCAGGGCCAACGCACGGGGCGTAGGCACCAGGCCACGGCCGGCGCGCACCAGGATCGGGTCACCCAAGGCATCGCGGATACGCCCCAATGTCCGGCTCATGGCCGCCGGGCTCAGGTTCATG                                                                                                                                                            <  8:191670/148‑1  TCCACCAGGCCATGCACCTGCTCGCGCAGGGCCAACGCACGGGGCGTAGGCACCAGGCCACGGCCGGCGCGCACCAGGATCGGGTCACCCAAGGCATCGCGGATACGCCCCAATGTCCGGCTCATGGCCGCCGGGCTCAGGTTCATGCG                                                                                                                                                          >  4:271651/1‑149    CACCAGGCCATGCACCTGCTCGCGCAGGGCCAACGCACGGGGCGTAGGCACCAGGCCACGGCCGGCGCGCACCAGGATCGGGTCCCCCAAGGCGTGGCGGATACGCCCGCGTGTCCGGCGCATGGCCGCGGGGCTCCGGGTCGTGCGCg                                                                                                                                                        >  8:210352/1‑148     ACCAGGCCATGCACCTGCTCGCGCAGGGCCAACGCACGGGGCGTAGGCACCAGGCCACGGCCGGCGCGCACCAGGATCGGGTCACCCAAGGCATCGCGGATACGCCCCAATGTCCGGCTCATGGCCGCCGGGCTCAGGTTCATGCGCTG                                                                                                                                                       <  5:168388/149‑1                           GCAGGGCCAACGCACGGGGCGTAGGCACCAGGCCACGGCCGGCGCGCACCAGGATCGGGTCACCCAAGGCATCGCGGATACGCCCCAATGTCCGGCTCATGGCCGCCGGGCTCAGGTTCATGCGCTGCGCCCACCACGCTGCCCTCGTC                                                                                                                                 <  6:38096/149‑1                                                                            CAGGATCGGGTCACCCAAGGCATCGCGGATACGCCCCAATGTCCGGCTCATGGCCGCCGGGCTCAGGTTCATGCGCTGCGCCCACCACGCTGCCCTCGTCGAGCAAGGCATC                                                                                                                     >  7:271879/1‑112                                                                            CAGGATCGGGTCACCCAAGGCATCGCGGATACGCCCCAATGTCCGGCTCATGGCCGCCGGGCTCAGGTTCATGCGCTGCGCCCACCACGCTGCCCTCGTCGAGCAAGGCATC                                                                                                                     <  8:271879/112‑1                                                                                                            GCCCCAATGTCCGGCTCATGGCCGCCGGGCTCAGGTTCATGCGCTGCGCCCACCACGCTGCCCTCGTCGAGCAAGGCATCGAGGGCGACGAGCAGGTTCATGTCCGGGAGTTGCATGGCCGTTTTCCGTTACAGCTGCGGGAAACGCGA                                                >  5:302194/1‑149                                                                                                                                                          CGCCCACCACGCTGCCCTCGTCGAGCAAGGCATCGAGGGCGACGAGCAGGTTCATGTCCGGGAGTTGCATGGCCGTTTTCCGTTACAGCTGCGGGAAACGCGATGGTAGCAGGTTGGTGGATTGCATCAGACGCAATGCGCTCTTGCGT  <  2:191496/149‑1                                                                                                                                                                                                                                                                                                                CTCCACCAGGCCATGCACCTGCTCGCGCAGGGCCAACGCACGGGGCGTAGGCACCAGGCCACGGCCGGCGCGCACCAGGATCGGGTCACCCAAGGCATCGCGGATACGCCCCAATGTCCGGCTCATGGCCGCCGGGCTCAGGTTCATGCGCTGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_002947/1451044‑1450889 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgcgctgcgcCCACCACGCTGCCCTCGTCGAGCAAGGCATCGAGGGCGACGAGCAGGTTCATGTCCGGGAGTTGCATGGCCGTTTTCCGTTACAGCTGCGGGAAACGCGATGGTAGCAGGTTGGTGGATTGCATCAGACGCAATGCGCTCTTGCGT  <  NC_002947/1450883‑1450738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.