New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 1375310	58 (1.190)	4 (0.080)	3/254	NT	7.0%	coding (930/1911 nt)	kup	potassium transport system protein Kup
	?	NC_002947	= 1375342	50 (1.050)					coding (962/1911 nt)	kup	potassium transport system protein Kup

ATTACTTCGGCCAGGGTGCATTGCTGCTGCAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAGGGGCAGATCTACATTGGTGCGGTGAACTGGACGCTGATGGTGGGCGTGGTGTT  >  NC_002947/1375195‑1375477                                                                                                                                                     |                                                                                                                                        aTTACTTCGGCCAGGGTGCATTGCTGCTGCAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGg                                                                                                                                        >  4:272560‑M2/1‑148 (MQ=255)     cTTCGGCCAGGGTGCATTGCTGCTGCAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGggcc                                                                                                                                     >  3:95446‑M2/1‑144 (MQ=255)      ttCGGCCAGGGTGCATTGCTGCTGCAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATAGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCtt                                                                                                                                   >  6:78077/1‑149 (MQ=255)          gCCAGGGTGCATTGCTGCTGCAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTcc                                                                                                                               >  6:279689/1‑149 (MQ=255)          gCCAGGGTGCATTGCTGCTGCAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTcc                                                                                                                               >  4:117680/1‑149 (MQ=255)          gCCAGGGTGCATTGCTGCTGCAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTcc                                                                                                                               >  2:284497/1‑149 (MQ=255)          gCCAGGGTGCATTGCTGCTGCAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATAGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTcc                                                                                                                               <  5:78077/149‑1 (MQ=255)          gCCAGGGTGCATTGCTGCTGCAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGGTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTcc                                                                                                                               <  8:198895/149‑1 (MQ=255)               ggTGCATTGCTGCTGCAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGAc                                                                                                                          >  6:54866/1‑149 (MQ=255)                 tGCATTGCTGCTGCAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGAccc                                                                                                                        >  4:276665/1‑149 (MQ=255)                     ttGCTGCTGCAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGc                                                                                                                      >  5:86708/1‑147 (MQ=255)                        ctgctgcAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTACTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGc                                                                                                                 <  1:288317/149‑1 (MQ=255)                        ctgctgcAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTACTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGc                                                                                                                 <  1:292892/149‑1 (MQ=255)                             gcAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATc                                                                                                             >  6:242332/1‑148 (MQ=255)                               aaaaCCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAg                                                                                                          <  5:65647/149‑1 (MQ=255)                                    ccGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTggg                                                                                                     >  3:46835/1‑149 (MQ=255)                                    ccGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTggg                                                                                                     <  1:47984/149‑1 (MQ=255)                                     cGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGc                                                                                                    <  3:117680/149‑1 (MQ=255)                                         ggCCTCGCGCAATCCGTGCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGTCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCtata                                                                                                <  7:127986/149‑1 (MQ=255)                                         ggCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCtata                                                                                                <  8:118083/149‑1 (MQ=255)                                               gcgcAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCcgc                                                                                          >  1:127314/1‑149 (MQ=255)                                                   aaTCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCAt                                                                                      <  6:86708/149‑1 (MQ=255)                                                      ccGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGAGCATGCa                                                                                   >  5:90198/1‑149 (MQ=255)                                                            tATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGAtt                                                                               >  7:235310/1‑147 (MQ=255)                                                              tCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTc                                                                              <  5:279689/146‑1 (MQ=255)                                                              tCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAg                                                                            <  1:284497/148‑1 (MQ=255)                                                                tgctgGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCCGACGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGca                                                                          >  1:91697/1‑149 (MQ=255)                                                                  ctgGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCacac                                                                       <  8:235310/149‑1 (MQ=255)                                                                  ctgGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCacac                                                                       >  7:81470/1‑149 (MQ=255)                                                                     gcgcCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCt                                                                     <  1:204524/148‑1 (MQ=255)                                                                     gcgcCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTc                                                                    <  4:46835/149‑1 (MQ=255)                                                                        ccGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAg                                                                 <  5:242332/149‑1 (MQ=255)                                                                             cTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGcac                                                                         <  5:308311/136‑1 (MQ=255)                                                                             cTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGcac                                                                         >  6:308311/1‑136 (MQ=255)                                                                                  cgcTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAg                                                          >  6:263775/1‑146 (MQ=255)                                                                                  cgcTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCa                                                        <  5:263775/148‑1 (MQ=255)                                                                                       ctgcCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAGGGGc                                                   <  3:276665/148‑1 (MQ=255)                                                                                        tgcCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAgggg                                                    >  1:271337/1‑146 (MQ=255)                                                                                        tgcCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAGGGGCAg                                                 <  2:127314/149‑1 (MQ=255)                                                                                          ccGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAgg                                                      <  8:283922/142‑1 (MQ=255)                                                                                          ccGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAgg                                                      >  7:283922/1‑142 (MQ=255)                                                                                            gTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAGGGGCAGATc                                              >  1:245099/1‑148 (MQ=255)                                                                                                   gggCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGAATGCAGATTCAGCACACCTCAAGCGACGAGCAGGGGCAGATCTACATTgg                                      >  6:179200/1‑149 (MQ=255)                                                                                                     gCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAGGGGCAGATCTACATTGGTg                                    >  2:86318/1‑149 (MQ=255)                                                                                                     gCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCCCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAGGGGCAGATCTACATTGGTg                                    >  5:59699/1‑149 (MQ=255)                                                                                                          ccaccaTGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGcgacg                                                            >  8:3231/1‑120 (MQ=255)                                                                                                          ccaccaTGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGcgacg                                                            <  7:3231/120‑1 (MQ=255)                                                                                                           caccaTGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGggccatggtggccagcccgaccaacggcagcagcgcccagcccggcgccagcagatagaacggattgcgcgcggcctccgggttttgcagcagcaatgcaccctggc                              <  3:285862‑M2/149‑108 (MQ=255)                                                                                                              caTGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGggccatggtggccagcccgaccaacggcagcagcgcccagcccggcgccagcagatagaacggattgcgcgcggcctccgggttttgcagcagcaatgcaccctggccg                            <  8:13044‑M2/148‑110 (MQ=255)                                                                                                              caTGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGggccatggtggccagcccgaccaacggcagcagcgcccagcccggcgccagcagatagaacggatt                                                                       >  6:175316‑M2/1‑39 (MQ=255)                                                                                                              caTGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGggccatggtggccagcccgaccaacggcagcagcgcccagcccggcgccagcagatagaacggatt                                                                       <  5:175316‑M2/105‑67 (MQ=255)                                                                                                                 ggCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAGGGGCAGATGTACATTGGTGCGGTGAACTg                          >  2:113552/1‑147 (MQ=255)                                                                                                                   ccACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAGGGGCAGATCTACATTGGTGCGGTGAACTGGACg                      <  5:54866/149‑1 (MQ=255)                                                                                                                         ttATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAGGGGCAGATCTACATTGGTGCGGTGAACTGGACGCTGAtg                <  2:91697/149‑1 (MQ=255)                                                                                                                               cc‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAGGGGCAGATCTACATTGGTGCGGTGAACTGGACGCTGATGGTGGGc          <  1:86318/149‑1 (MQ=255)                                                                                                                                      aGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAGGGGCAGATCTACATTGGTGCGGTGAACTGGACGCTGATGGTGGGCgtggtg    <  2:271337/149‑1 (MQ=255)                                                                                                                                        gCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAGGGGCAGATCTACATTGGTGCGGTGAACTGGACGCTGATGGTGGGCGTGGTGtt  >  4:35676/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                        ATTACTTCGGCCAGGGTGCATTGCTGCTGCAAAACCCGGAGGCCGCGCGCAATCCGTTCTATCTGCTGGCGCCGGGCTGGGCGCTGCTGCCGTTGGTCGGGCTGGCCACCATGGCCACGGTTATCGCC‑TCGCAGGCGGTGATTTCCGGGGCCTTCTCCCTGACCCGCCAGGCCATCCAGCTGGGCTATATTCCGCGCATGCAGATTCAGCACACCTCAAGCGACGAGCAGGGGCAGATCTACATTGGTGCGGTGAACTGGACGCTGATGGTGGGCGTGGTGTT  >  NC_002947/1375195‑1375477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.