Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,763,178 T→C 8.5% Y172Y (TAT→TAC)  pvdO → pyoverdine biosynthesis‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,763,1780TC 8.5% 129.5 / 2.4 47Y172Y (TAT→TAC) pvdOpyoverdine biosynthesis‑like protein
Reads supporting (aligned to +/- strand):  ref base T (20/23);  new base C (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TCTGCGCCGCCAGCAAGCCGACCTACAAACAGGGCCCGCGCCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAACACCTACGGCCCCACCGACGGTTACTCGTACACCGCCCCGGTCGGCAGCTACCCGG  >  NC_002947/4763030‑4763320
                                                                                                                                                    |                                                                                                                                               
tCTGCGCCGCCAGCAAGCCGACCTACAAACAGGGCCCGCGCCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAatat                                                                                                                                                 >  7:57171/1‑149 (MQ=255)
  tGCGCCGCCAGCAAGCCGACCTACAAACAGGGCCCGCGCCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGc                                                                                                                                               >  3:265730/1‑149 (MQ=255)
   gcgcCGCCAGCAAGCCGACCTACAAACAGGGCCCGCGCCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGcc                                                                                                                                              >  5:19844/1‑149 (MQ=255)
        gccAGCAAGCCGACCTACAAACAGGGCCCGCGCCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGTGAAGCCGAGCGCGAATACGCcgcgcg                                                                                                                                         >  2:114180/1‑149 (MQ=255)
        gccAGCAAGCCGACCTACAAACAGGGCACGCGCCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGTGAAGCCGAGCGCGAATACGCcgcgcg                                                                                                                                         >  8:229753/1‑149 (MQ=255)
                   ctacctcAAACAGGGCCCGCGCCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCaa                                                                                                                             <  3:84826/143‑1 (MQ=255)
                    aCCTACAAACAGGGCCCGCTCCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAAc                                                                                                                            <  5:116294/149‑1 (MQ=255)
                           aaCAGGGCCCGCGCCAGCCTGCCGTGTGCGTGAGCTGGTTCCATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCg                                                                                                                     >  7:249312/1‑149 (MQ=255)
                                    cgcgCCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGttccccttc                                                                                                            <  8:57171/149‑1 (MQ=255)
                                    cgcgCCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGttccccttc                                                                                                            <  1:232618/149‑1 (MQ=255)
                                    cgcgCCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGttccccttc                                                                                                            <  8:47843/149‑1 (MQ=255)
                                    cgcgCCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGttccccttc                                                                                                            <  4:133265/149‑1 (MQ=255)
                                     gcgcCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCGCCGCCGGTTCAACCGGCCCGttccccttc                                                                                                            <  1:18088/149‑1 (MQ=255)
                                       gcCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGttccccttcccc                                                                                                         >  8:285298/1‑149 (MQ=255)
                                         cAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTccccttcccctt                                                                                                       >  1:266958/1‑149 (MQ=255)
                                          aGCCTGTCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCcccttccccttc                                                                                                      <  1:265490/149‑1 (MQ=255)
                                             cTGCCGTGTGCGTGAGCTGGTTCCATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGAt                                                                                                   <  8:249312/149‑1 (MQ=255)
                                                        gTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGc                                                                                        <  3:224889/149‑1 (MQ=255)
                                                            gCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTcc                                                                                    <  5:105900/149‑1 (MQ=255)
                                                             cTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCa                                                                                   <  6:19844/149‑1 (MQ=255)
                                                                gTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCa                                                                                   <  2:266958/146‑1 (MQ=255)
                                                                gTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAgg                                                                                 >  3:237895/1‑148 (MQ=255)
                                                                          gAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGc                                                                      >  6:268299/1‑149 (MQ=255)
                                                                          gAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGc                                                                      >  5:309574/1‑149 (MQ=255)
                                                                              aaTACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAg                                                                  <  6:309574/149‑1 (MQ=255)
                                                                                tACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCATTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCa                                                                >  1:267419/1‑149 (MQ=255)
                                                                                 aCGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACa                                                                             >  6:147086/1‑135 (MQ=255)
                                                                                 aCGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACa                                                                             <  5:147086/135‑1 (MQ=255)
                                                                                   gCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCACGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGc                                                             >  3:246288/1‑149 (MQ=255)
                                                                                       aGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGTGAAGCCGAGCGCGAATACGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAac                                                         <  1:114180/149‑1 (MQ=255)
                                                                                       aGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGTGAAGCCGAGCGCGAATACGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAac                                                         <  7:229753/149‑1 (MQ=255)
                                                                                             tGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAaca                                                        <  7:267705/144‑1 (MQ=255)
                                                                                             tGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAACACCTAc                                                   >  2:266856/1‑149 (MQ=255)
                                                                                             tGTCGAAAAAAACCGGCCACAACTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAaca                                                        >  8:267705/1‑144 (MQ=255)
                                                                                                 gAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAACACCTACGGcc                                               <  4:265730/149‑1 (MQ=255)
                                                                                                                 acCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAACACCTACGGCCCCACCGACGGTTACTc                               <  1:266856/149‑1 (MQ=255)
                                                                                                                 acCTATCGCACGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAACACCTACGGCCCCACCGACGGTTACTc                               <  4:246288/149‑1 (MQ=255)
                                                                                                                   cTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCATCCAGGTACAGCATCGCCCATCACGCAAACACCTACGGCCCCACCCACCGTTAATCg                              >  8:282131/1‑148 (MQ=255)
                                                                                                                            gATAAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAACACCTACGGCCCCACCGACGGTTACTCGTACACCGccc                    >  3:302004/1‑149 (MQ=255)
                                                                                                                               cAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAg                                                                  <  8:21538/100‑1 (MQ=255)
                                                                                                                               cAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAg                                                                  >  7:21538/1‑100 (MQ=255)
                                                                                                                                aGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAACACCTACGGCCCCACCGACGGTTACTCGTACACCGCCCCgg                 <  5:268299/148‑1 (MQ=255)
                                                                                                                                 gCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAACACCTACGGCCCCACCGACGGTTACTCGTACACCGCCCCGGTc               >  5:42476/1‑149 (MQ=255)
                                                                                                                                      gCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAACACCTACGGCCCCACCGACGGTTACTCGTACACCGCCCCGGTCGGCAg          <  7:285298/149‑1 (MQ=255)
                                                                                                                                       ccGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAACACCTACGGCCCCACCGACGGTTACTCGTACACCGCCCCGGTCGGCAGc         >  7:88539/1‑149 (MQ=255)
                                                                                                                                        cGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAACACCTACGGCCCCACCGACGGTTACTCGTACACCGCCCCGGTCGGCAGc         >  3:145018/1‑148 (MQ=255)
                                                                                                                                               gAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAACACCTACGGCCCCACCGACGGTTACTCGTACACCGCCCCGGTCGGCAGCTACCCgg  <  8:224135/148‑1 (MQ=255)
                                                                                                                                               gAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAACACCTACGGCCCCACCGACGGTTACTCGTACACCGCCCCGGTCGGCAGCTACCCgg  >  7:224135/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TCTGCGCCGCCAGCAAGCCGACCTACAAACAGGGCCCGCGCCAGCCTGCCGTGTGCGTGAGCTGGTTCGATGTCGAGCAATACGCCAAGTGGCTGTCGAAAAAAACCGGCCACACCTATCGCATGATCAGCGAAGCCGAGCGCGAATATGCCGCGCG‑CGCCGGTTCAACCGGCCCGTTCCCCTTCCCCTTCGATAACGGCGACGCCTCCAGGTACAGCATCGCCCAGCACGCCAACACCTACGGCCCCACCGACGGTTACTCGTACACCGCCCCGGTCGGCAGCTACCCGG  >  NC_002947/4763030‑4763320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑