Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,793,958 C→T 10.3% intergenic (‑119/+37) pvdI ← / ← PP_4222 non‑ribosomal peptide synthetase/protein SyrP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,793,9580CT10.3% 158.5 / 6.6 58intergenic (‑119/+37)pvdI/PP_4222non‑ribosomal peptide synthetase/protein SyrP
Reads supporting (aligned to +/- strand):  ref base C (29/23);  new base T (4/2);  total (33/25)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTGCGCCGCGAGCATGTTGTCGAGCATCAGCACG  >  NC_002947/4793810‑4794103
                                                                                                                                                    |                                                                                                                                                  
aGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGATCGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAgc                                                                                                                                                    <  5:152465/149‑1 (MQ=255)
     gTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTg                                                                                                                                               >  1:32045/1‑149 (MQ=255)
      tttGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGc                                                                                                                                              >  6:100777/1‑149 (MQ=255)
      tttGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGc                                                                                                                                              <  2:295300/149‑1 (MQ=255)
        tgagtgagTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGGTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCg                                                                                                                                             <  5:246384/148‑1 (MQ=255)
            tgagtCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGTGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAg                                                                                                                                        >  5:242950/1‑149 (MQ=255)
                            aTGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGTGCTTGCGGCTTGCC‑GCAGGCGCTCGCca                                                                                                                       <  5:281697/149‑1 (MQ=39)
                                  ggggTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTg                                                                                                                  <  3:67972/148‑1 (MQ=255)
                                             tGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAgcgc                                                                                                      >  7:111462/1‑149 (MQ=255)
                                             tGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAgcgc                                                                                                      >  4:181000/1‑149 (MQ=255)
                                             tGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGC‑GCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGcgcc                                                                                                     >  4:128790/1‑149 (MQ=255)
                                              gATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCGCAAAGcgcc                                                                                                     >  7:252939/1‑149 (MQ=255)
                                                   gCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTc                                                                                                >  6:135110/1‑149 (MQ=255)
                                                     gctttggCCGCACGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTcgc                                                                                              >  4:81233/6‑149 (MQ=255)
                                                     aGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTcgc                                                                                              >  2:26822/1‑149 (MQ=255)
                                                      gCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTcgcg                                                                                             <  7:4036/149‑1 (MQ=255)
                                                       cTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCgg                                                                                            >  6:308533/1‑149 (MQ=255)
                                                        tCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGt                                                                                           >  5:294109/1‑149 (MQ=255)
                                                          ggCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTg                                                                                          <  5:100777/148‑1 (MQ=255)
                                                           gCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGcc                                                                                        >  1:177031/1‑149 (MQ=255)
                                                                  ggCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTcgcg                                                                                             >  5:87059/1‑137 (MQ=255)
                                                                  ggCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTcgcg                                                                                             <  6:87059/137‑1 (MQ=255)
                                                                    ccAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGC‑GCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCAt                                                                              <  3:128790/149‑1 (MQ=255)
                                                                               ccAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGAc                                                                     <  8:111462/148‑1 (MQ=255)
                                                                                cAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCa                                                                   >  4:245010/1‑149 (MQ=255)
                                                                                 aGTAGAACGAGCGGAGGGAGGGCGAATTTAAAGCGGCAGCGCCATGCCCGGACGCTGCGATGGCGAGCGCCTGAGGCAAGCC‑GCAAGCAATAGCCACAGTGGCCTCAAAGCGACGCTTCGCGGTGCCACTGAGCAATGGCCAAGACCAc                                                                  >  6:216791/1‑149 (MQ=255)
                                                                                   tAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACtt                                                                <  3:181000/149‑1 (MQ=255)
                                                                                      aaCGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGc                                                             >  1:80982/1‑149 (MQ=255)
                                                                                          aGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTgg                                                         >  7:71714/1‑149 (MQ=255)
                                                                                          aGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTgg                                                         <  2:177031/149‑1 (MQ=255)
                                                                                           gCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGc                                                        >  7:161953/1‑149 (MQ=255)
                                                                                            cGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGcc                                                       <  6:294109/149‑1 (MQ=255)
                                                                                               agggagggCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCgg                                                    <  6:242950/149‑1 (MQ=255)
                                                                                                  gagggCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTg                                                  >  1:166242/1‑148 (MQ=255)
                                                                                                      gCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATg                                                                             >  7:131685/1‑117 (MQ=255)
                                                                                                      gCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATg                                                                             <  8:131685/117‑1 (MQ=255)
                                                                                                       cGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGt                                            >  1:241047/1‑149 (MQ=255)
                                                                                                        gAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACg                                              >  6:288829/1‑146 (MQ=255)
                                                                                                        gAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACg                                              <  5:288829/146‑1 (MQ=255)
                                                                                                              aaCGCGGCAGCGCCATGCCCGGACGCTGCGCTGTCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCggg                                     >  3:63139/1‑149 (MQ=255)
                                                                                                              aaCGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCgg                                      <  5:308533/148‑1 (MQ=255)
                                                                                                              aaCGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCgg                                      <  2:80982/148‑1 (MQ=255)
                                                                                                                  cgGCAGCGCCATGCCCGGACGCTGCGCTGTCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTg                                 <  4:63139/149‑1 (MQ=255)
                                                                                                                   ggCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTgc                                <  8:71714/149‑1 (MQ=255)
                                                                                                                    gCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCCGGCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTgc                                >  1:67442/1‑149 (MQ=255)
                                                                                                                    gCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACttt                                                               >  4:168321/1‑117 (MQ=255)
                                                                                                                           cATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTGCGCCGCGAg                        >  1:41631/1‑149 (MQ=255)
                                                                                                                               cccGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTGCGCCGCGAGCAtg                    <  2:32045/149‑1 (MQ=255)
                                                                                                                                     cgctgcgctgGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTGCGCCGCGAGCATGTTGTCg              <  5:135110/149‑1 (MQ=255)
                                                                                                                                      gctgcgctgGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTGCGCCGCGAGCATGTTGTCGa             <  8:161953/149‑1 (MQ=255)
                                                                                                                                      gccactgtgGCGAGTGCTTGCGGCTTGCC‑GCAGGCGCTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTGCGCCGCGAGCATGTTGTc               >  3:254486/8‑147 (MQ=38)
                                                                                                                                        cactgtgGCGAGTGCTTGCGGCTTGCC‑GCAGGCGCTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTGCGCCGCGAGCATGTTGTCGAg            >  3:242919/6‑148 (MQ=38)
                                                                                                                                              ggCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTGCGCCGCGAGCATGTTGTCGAGCATCAGc     <  3:245010/149‑1 (MQ=255)
                                                                                                                                               gCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTGCGCCGCGAGCATGTTGTCGAGCATCAGCa    >  4:94625/1‑149 (MQ=255)
                                                                                                                                                 gAGTGCTTGCGGCTTGCC‑GCAGGCGCTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTGcgccgc                           <  2:116583/124‑1 (MQ=38)
                                                                                                                                                 gAGTGCTTGCGGCTTGCC‑GCAGGCGCTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTGCGCCGCGAGCATGTTGTCGAGCATCAGCAcg  >  1:2278/1‑149 (MQ=255)
                                                                                                                                                 gAGTGCTTGCGGCTTGCC‑GCAGGCGCTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTGCGCCGCGAGCATGTTGTCGAGCATCAGCAcg  >  3:27512/1‑149 (MQ=255)
                                                                                                                                                 gAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGc                                          >  6:283532/1‑109 (MQ=255)
                                                                                                                                                 gAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGc                                          <  5:283532/109‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCC‑GCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCGGTGCCCCTGAGCCATGGCCACGACCACTTTGCGTGGCCCGGTGAACGGTGCCCGGGCGTGCGCCGCGAGCATGTTGTCGAGCATCAGCACG  >  NC_002947/4793810‑4794103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑