Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,796,069 G→A 7.4% A125V (GCA→GTA)  pvdH ← diaminobutyrate‑2‑oxoglutarate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,796,0690GA 7.4% 158.8 / 2.6 54A125V (GCA→GTA) pvdHdiaminobutyrate‑2‑oxoglutarate transaminase
Reads supporting (aligned to +/- strand):  ref base G (22/28);  new base A (2/2);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

CGAGCAAGGCCCCCAGTGGCTGCTTCGGCCCATGGCTGCCCATCAGGTTCAGCGCGCCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCGGGGTGGTCAGGTCCAAGGTGTGCAGCGGCAACTCGTCAGCCAGCACC  >  NC_002947/4795923‑4796215
                                                                                                                                                  |                                                                                                                                                  
cGAGCAAGGCCCCCAGTGGCTGCTTCGGCCCATGGCTGCCCATCAGGTTCAGCGCGCCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCa                                                                                                                                                  <  2:182455/149‑1 (MQ=255)
cGAGCAAGGCCCCCAGTGGCTGCTTCGGCCCATGGCTGCCCATCAGGTTCAGCGCGCCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCa                                                                                                                                                  >  5:303318/1‑149 (MQ=255)
cGAGCAAGGCCCCCAGTGGCTGCTTCGGCCCATGGCTGCCCATCAGGTTCAGCGCGCCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCa                                                                                                                                                  >  5:214543/1‑149 (MQ=255)
 gAGCAAGGCCCCCAGTGGCTGCTTCGGCCCATGGCTGCCCATCAGGTTCAGCGCGCCTTGGCTCATGCCATGGTCGGCGCCCGGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCAt                                                                                                                                                 <  8:195372/149‑1 (MQ=255)
 gAGCAAGGCCCCCAGTGGCTGCTTCGGCCCATGGCTGCCCATCAGGTTCAGCGCGCCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCAt                                                                                                                                                 <  6:214543/149‑1 (MQ=255)
    cAAGGCCCCCAGTGGCTGCTTCGGCCCATGGCTGCCCATCAGGTTCAGCGCGCCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAg                                                                                                                                              >  7:130864/1‑149 (MQ=255)
                tGGCTGCTTCGGCCCATGGCTGCCCATCAGGTTCAGCGCGCCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGc                                                                                                                                  <  6:270612/149‑1 (MQ=255)
                tGGCTGCTTCGGCCCATGGCTGCCCATCAGGTTCAGCGCGCCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGc                                                                                                                                  <  6:107961/149‑1 (MQ=255)
                       ttCGGCCCATGGCTGCCCATCAGGTTCAGCGCGCCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGaa                                                                                                                           >  2:44722/1‑149 (MQ=255)
                           gCCCATGGCTGCCCATCAGGTTCAGCGCGCCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTg                                                                                                                        >  6:28161/1‑148 (MQ=255)
                            cccATGGCTGCCCATCAGGTTCAGCGCGCCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCa                                                                                                                      >  6:30058/1‑149 (MQ=255)
                                         aTCAGGTTCAGCGCGCCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGAACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTcgcg                                                                                                         >  4:267794/1‑149 (MQ=255)
                                                   gcgcgcCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGcc                                                                                               <  3:267794/149‑1 (MQ=255)
                                                    cgcgcCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCAcc                                                                                                                    <  7:255451/127‑1 (MQ=255)
                                                    cgcgcCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCAcc                                                                                                                    >  8:255451/1‑127 (MQ=255)
                                                          ttGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCggg                                                                                          >  4:57827/1‑147 (MQ=255)
                                                              cTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGAt                                                                                    <  5:258325/149‑1 (MQ=255)
                                                              cTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTACACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGAt                                                                                    <  8:120502/149‑1 (MQ=255)
                                                               tCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATg                                                                                   >  7:63953/1‑149 (MQ=255)
                                                                 aTGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGcc                                                                                 <  5:30058/149‑1 (MQ=255)
                                                                       tGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAg                                                                           <  1:44722/149‑1 (MQ=255)
                                                                        ggTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAgg                                                                          >  3:224467/1‑149 (MQ=255)
                                                                         gTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAgg                                                                          <  8:13289/148‑1 (MQ=255)
                                                                             gcgcCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGCGCGCCGCCTCGACGGCATCAGTGCCGGGCGGGCCACAGAACTGCACCGTGGCCTCGCGGCGCCGCGCCGCGGGCAGGATGCCGCACAGGTCCTg                                                                     >  5:93176/1‑149 (MQ=255)
                                                                             gcgcCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGt                                                                         <  3:64928/145‑1 (MQ=255)
                                                                             gcgcCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGt                                                                         >  4:64928/1‑145 (MQ=255)
                                                                             gcgcCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTg                                                                     >  5:81183/1‑149 (MQ=255)
                                                                               gcCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAg                                                                           <  8:250049/141‑1 (MQ=255)
                                                                               gcCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAg                                                                           >  7:250049/1‑141 (MQ=255)
                                                                               gcCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGa                                                                   <  6:81183/149‑1 (MQ=255)
                                                                                cccTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGaa                                                                              <  7:96879/137‑1 (MQ=255)
                                                                                cccTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGaa                                                                              >  8:96879/1‑137 (MQ=255)
                                                                                     gAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGaa                                                               <  1:28539/147‑1 (MQ=255)
                                                                                     gAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGc                                                             >  6:44202/1‑149 (MQ=255)
                                                                                      aaGGCCAGCACGGTGCGGCGGCCGGTTGCGGGGCGCACCAGCTTGAGCGCAGCCTCGACTGCAGCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCg                                                            <  6:93176/149‑1 (MQ=255)
                                                                                        ggCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCGGt                                                          <  5:44202/149‑1 (MQ=255)
                                                                                              cACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCGGTCCTTGa                                                    <  3:57827/149‑1 (MQ=255)
                                                                                                 ggTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAgg                                                                                      <  5:255604/112‑1 (MQ=255)
                                                                                                 ggTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAgg                                                                                      >  6:255604/1‑112 (MQ=255)
                                                                                                     ctgcGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCgggg                                              <  6:303318/148‑1 (MQ=255)
                                                                                                     ctgcGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCgggg                                              <  8:130864/148‑1 (MQ=255)
                                                                                                         ggCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGcc                                                                                 <  1:267670/109‑1 (MQ=255)
                                                                                                         ggCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGcc                                                                                 >  2:267670/1‑109 (MQ=255)
                                                                                                           ccGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCGGGGTGGTCAg                                       <  8:63953/149‑1 (MQ=255)
                                                                                                            cGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTACATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGTTGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCGGggtggt                                          <  1:213442/145‑1 (MQ=255)
                                                                                                            cGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTACATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCGGggtggt                                          >  4:207101/1‑145 (MQ=255)
                                                                                                            cGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTACATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCGGggtggt                                          >  2:213442/1‑145 (MQ=255)
                                                                                                            cGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTACATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCGGggtggt                                          <  3:207101/145‑1 (MQ=255)
                                                                                                             ggTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCGGGGTGGTCAGGt                                     <  2:302323/149‑1 (MQ=255)
                                                                                                              gTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGTTGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCGGggtggt                                          >  5:181041/1‑143 (MQ=255)
                                                                                                              gTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGTTGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCGGggtggt                                          <  6:181041/143‑1 (MQ=255)
                                                                                                                 gCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCGGGGTGGTCAGGTcc                                   >  4:298040/1‑147 (MQ=255)
                                                                                                                  cGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCGGGGTGGTCAGGTCCAAg                                <  4:224467/149‑1 (MQ=255)
                                                                                                                                                cTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCGGGGTGGTCAGGTCCAAGGTGTGCAGCGGCAACTCGTCAGCCAGCAcc  >  7:240443/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGAGCAAGGCCCCCAGTGGCTGCTTCGGCCCATGGCTGCCCATCAGGTTCAGCGCGCCTTGGCTCATGCCATGGTAGGCGCCCTGGAAGGCCAGCACGGTGCTGCGGCCGGTGGCGGTGCGCACCAGCTTGAGCGCAGCCTCGACTGCATCAGTGCCGGTCGGGCCACAGAACTGCACCTTGGCCTCGCGGCGCAGCGCCTCGGGCAGGATGCCGAACAGGTCCTGGACGAAGCGGTCCTTGACCGGGGTGGTCAGGTCCAAGGTGTGCAGCGGCAACTCGTCAGCCAGCACC  >  NC_002947/4795923‑4796215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑