| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,796,619 | A→G | 19.7% | intergenic (‑177/+41) | pvdH ← / ← PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,796,619 | 0 | A | G | 19.7% | 109.2 / 21.6 | 57 | intergenic (‑177/+41) | pvdH/PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base A (17/28); new base G (7/4); total (24/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01 | |||||||||||
GCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGC > NC_002947/4796474‑4796759 | gCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGG‑AACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCgg < 2:9046/149‑1 (MQ=255) cGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTGGAGTGGGGGTAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCgg < 7:163644/149‑1 (MQ=255) cGGTGGAAATGGGTAAATCCTCGCGATCAAGCGGATGGGCTGTTATCGCTTTGTAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCgg < 3:229188/149‑1 (MQ=255) ggTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTAcgggcggg < 3:263998/149‑1 (MQ=38) ggTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCggg < 6:312217/149‑1 (MQ=255) ggACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTc < 4:266032/149‑1 (MQ=255) aattGGGTTATTCCTCGCGAGCAAGTGGATGTGCGGTTATGGCTTTGGTTACGCTTGAGTGGGGGGAGGA‑TTAGCGGTTGTTTGGCGATTTTTTTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGTGCGTGTTCAc < 6:26491/145‑1 (MQ=37) cATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAcc < 3:102097/149‑1 (MQ=255) aTGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAccc < 3:237659/149‑1 (MQ=38) aTGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAccc > 4:237659/1‑149 (MQ=38) aTGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAccc < 3:64820/149‑1 (MQ=255) gggTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCggg > 4:83627/1‑140 (MQ=255) gggTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCggg < 3:83627/140‑1 (MQ=255) aaTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGaa > 4:9254/1‑147 (MQ=255) aaTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAaga < 8:209238/149‑1 (MQ=255) cTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGc < 8:25034/148‑1 (MQ=255) cgcgAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGGTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAg < 3:63646/149‑1 (MQ=255) gCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTAACCCGCGAAGAGGCCAGCACAg > 3:250334/1‑149 (MQ=255) cAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAgg > 7:237142/1‑149 (MQ=255) gCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑a < 2:193440/149‑1 (MQ=255) gggCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAAc < 8:237142/148‑1 (MQ=255) tCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGccc < 7:20718/149‑1 (MQ=255) cTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTCCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATa < 1:124341/133‑1 (MQ=255) tttGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGGTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCAGCCCGTAGGAGCGGGTTCACCCGCCAAGAGGCCAGCACCGGCG‑ATCAACATCAATGCCCAGGa > 2:83755/1‑149 (MQ=39) gataCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑cgc < 8:92369/145‑1 (MQ=255) aCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCGCAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCg < 3:203624/149‑1 (MQ=255) cTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGCATAAACATCAATGCCCAGGA‑‑CGCGCCGGt < 1:22781/149‑1 (MQ=255) gggggAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGcaa < 3:9254/148‑1 (MQ=255) cgatctgcgATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCCAGCCGT‑Ca < 5:238136/143‑1 (MQ=255) cgATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAAAGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑Ca > 6:238136/1‑142 (MQ=255) gATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCt < 7:21660/149‑1 (MQ=255) tttttGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTGACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGC‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAg > 5:238140/1‑149 (MQ=255) ttatcgccTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGACG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCaaa < 5:122522/141‑1 (MQ=18) ttatcgccTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGACG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCa > 6:122522/8‑146 (MQ=18) ttGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAgcg < 3:268080/148‑1 (MQ=255) tgtgCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGc > 1:247181/1‑148 (MQ=255) tgCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGACCCGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAg < 1:83755/146‑1 (MQ=255) tGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCgg > 3:110106/1‑149 (MQ=255) ttCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCg > 5:64110/1‑149 (MQ=37) gggTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCa > 8:267097/1‑149 (MQ=255) ggTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTc > 8:10259/1‑119 (MQ=25) ggTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTc < 7:10259/119‑1 (MQ=25) gTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc > 3:256212/1‑148 (MQ=255) tgaaCCCGCTCCTACGGGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGa > 3:258864/3‑149 (MQ=255) tAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGcaa > 7:95748/1‑87 (MQ=255) tAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGcaa < 8:95748/87‑1 (MQ=255) tAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGcaa < 4:253706/87‑1 (MQ=255) tAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGcaa > 3:253706/1‑87 (MQ=255) gaaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGAt > 3:219976/2‑149 (MQ=38) gaaCCCGCTCCTACGGGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGc < 7:267585/143‑1 (MQ=255) gaaCCCGCTCCTACGGGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGc > 8:267585/2‑144 (MQ=255) aCCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCg > 6:99531/1‑149 (MQ=255) cccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATc < 4:169292/147‑1 (MQ=255) cccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATc > 3:169292/1‑147 (MQ=255) ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCCACTACGGCTGTCAAAGCGCAGGCTACCGGCacac < 1:196979/135‑1 (MQ=255) ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCacac > 2:196979/1‑134 (MQ=255) ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGcc > 5:267430/1‑149 (MQ=255) cccgTAGGAGCGGGTTCACCCGCTAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGc < 7:244505/149‑1 (MQ=255) | GCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCG‑ATAAACATCAATGCCCAGGA‑‑CGCGCCGGTACCTGCAACAAAACCCGCAAGCCGT‑CACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGC > NC_002947/4796474‑4796759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |