Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,826,563 C→T 7.7% A1543T (GCA→ACA)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,826,5630CT 7.7% 148.7 / 2.1 52A1543T (GCA→ACA) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (25/23);  new base T (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-01

CCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCGGGCGCGATCGCCACTGCGGTACAGACGCT  >  NC_002947/4826419‑4826700
                                                                                                                                                |                                                                                                                                         
ccGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCcag                                                                                                                                        >  5:45071/1‑148 (MQ=255)
   gCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAgcagg                                                                                                                                    >  6:206011/1‑149 (MQ=255)
    cACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAgcaggc                                                                                                                                   <  8:173703/149‑1 (MQ=255)
      ccATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAgcaggcag                                                                                                                                 <  6:94591/149‑1 (MQ=255)
         tGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGcaggcaggtg                                                                                                                              <  1:289320/149‑3 (MQ=255)
         tGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGcc                                                                                                                              >  6:174526/1‑149 (MQ=255)
                tGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACtt                                                                                                                       >  8:266012/1‑149 (MQ=255)
                         cTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCTCTTCCTCGGGc                                                                                                               <  7:25850/148‑1 (MQ=255)
                          tCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCcagc                                                                                                                                       >  3:37349/1‑123 (MQ=255)
                          tCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCcagc                                                                                                                                       <  4:37349/123‑1 (MQ=255)
                          tCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGGATCAGCACCCGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACGTCCTCGGGCTc                                                                                                             >  5:84358/1‑149 (MQ=255)
                            aGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCg                                                                                                            <  7:39221/148‑1 (MQ=255)
                                aGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGACACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGAcgcg                                                                                                       <  7:71072/149‑1 (MQ=255)
                                     ggCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAAc                                                                                                  >  6:151915/1‑149 (MQ=255)
                                            aCCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCca                                                                                                                                         >  8:153072/1‑103 (MQ=255)
                                            aCCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCca                                                                                                                                         <  7:153072/103‑1 (MQ=255)
                                              cTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCt                                                                                         <  5:174526/149‑1 (MQ=255)
                                               tCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGTCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCtt                                                                                        >  8:179362/1‑149 (MQ=255)
                                                cGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGTCAGCAGGCAGGCCTGCACTTCCTCg                                                                                                                  <  1:200659/122‑1 (MQ=255)
                                                cGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGTCAGCAGGCAGGCCTGCACTTCCTCg                                                                                                                  >  2:200659/1‑122 (MQ=255)
                                                 ggCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTg                                                                                       <  2:297054/148‑1 (MQ=255)
                                                          ggCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGt                                                                             <  7:266012/149‑1 (MQ=255)
                                                                     tGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACATCCACGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGcc                                                                   >  4:267848/1‑148 (MQ=255)
                                                                        aaTAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAgg                                                               >  8:82045/1‑149 (MQ=255)
                                                                          tAGCCGACCAGTTGCTCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTACACACCCGCCTGTGCCAgcagtcag                                                                                                                                 >  6:206985/1‑81 (MQ=255)
                                                                          tAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTa                                                             <  5:151915/149‑1 (MQ=255)
                                                                          tAGCCGACAAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCACAGCCTGTTCCACACCCGCCTGTGCCAgcaggcag                                                                                                                                 <  5:206985/81‑1 (MQ=255)
                                                                              cGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTcc                                                         >  3:201521/1‑149 (MQ=255)
                                                                               gACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCa                                                        <  4:63724/149‑1 (MQ=255)
                                                                                 ccAGTTGCGCGCCAATGGCGTCATTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCATTCCTTCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAgg                                                      <  2:212352/149‑1 (MQ=255)
                                                                                       gcgcgcCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTc                                                 >  1:78255/1‑148 (MQ=255)
                                                                                        cgcgcCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTcc                                                <  5:206011/148‑1 (MQ=255)
                                                                                          cgcCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGc                                             <  6:45071/149‑1 (MQ=255)
                                                                                           gcCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAACCCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCt                                            >  6:187298/1‑149 (MQ=255)
                                                                                             cAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACcg                                                                                                >  2:154571/1‑95 (MQ=255)
                                                                                                   cGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGc                                    >  6:245234/1‑149 (MQ=255)
                                                                                                          gtgtATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCg                               <  5:245234/147‑1 (MQ=255)
                                                                                                             tATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCGGgcg                           <  2:78255/148‑1 (MQ=255)
                                                                                                                  gCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAAc                                        >  2:195109/1‑130 (MQ=255)
                                                                                                                  gCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAAc                                        <  1:195109/130‑1 (MQ=255)
                                                                                                                  gCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCGGGCGCGATCg                     >  8:164572/1‑149 (MQ=255)
                                                                                                                   cACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCGGGCGCGATc                      >  1:136857/1‑147 (MQ=255)
                                                                                                                   cACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCGGGCGCGATc                      <  2:136857/147‑1 (MQ=255)
                                                                                                                         cgcCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCGGGCGCGATCGc                    <  5:91635/143‑1 (MQ=255)
                                                                                                                         cgcCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCGGGCGCGATCGc                    >  6:91635/1‑143 (MQ=255)
                                                                                                                              gTTCCACACCCGCCTGTGTCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCGGGCGCGATCGCCACTGCGGTAc         <  7:179362/149‑1 (MQ=255)
                                                                                                                               ttCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCGGGCGCGATCGCCACTGCGGTa          >  8:72328/1‑147 (MQ=255)
                                                                                                                                   acacCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGTGGGCGCGATCGCCACTGCGGTACAGACg    >  2:295705/1‑149 (MQ=255)
                                                                                                                                   acacCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCGGGCGCGATCGCCACTGCGGTACAGACg    >  5:279124/1‑149 (MQ=255)
                                                                                                                                    cacCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCGGGCGCGATCGCCACTGCgg            >  7:90471/1‑140 (MQ=255)
                                                                                                                                    cacCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCGGGCGCGATCGCCACTGCgg            <  8:90471/140‑1 (MQ=255)
                                                                                                                                     acCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCGGGCGCGATCGCCACTGCGGTACAGACGCt  >  6:72276/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                         
CCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGGCTCGACGCGGAAACCGCGCACCTTGACCTGCTGGTCGAGGCGGCCAAGGTATTCCAGGGCCTCCAGCTGAACCTGCCAGCGGGCGCGATCGCCACTGCGGTACAGACGCT  >  NC_002947/4826419‑4826700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑