| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,909,921 | C→G | 9.1% | intergenic (+314/+165) | PP_4318 → / ← PP_4319 | transposase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,909,921 | 0 | C | G | 9.1% | 131.5 / 7.6 | 55 | intergenic (+314/+165) | PP_4318/PP_4319 | transposase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (24/25); new base G (3/2); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01 | |||||||||||
CCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAAAGCAGAAGCAGAAGCAGAAGCAGCA > NC_002947/4909778‑4910064 | ccGCCCTTACGGCGGGTCACTTTTTGTTAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATgg > 2:176822/1‑149 (MQ=18) cGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATggg < 3:180369/149‑1 (MQ=32) cGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATgg < 7:155951/148‑1 (MQ=32) gCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTGCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATgggg > 1:252254/1‑149 (MQ=17) gCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATgggg > 8:200806/1‑149 (MQ=32) cTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCt > 5:79958/1‑149 (MQ=32) aCGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑Ca < 5:116718/148‑1 (MQ=32) cggcggGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAgc < 5:108966/149‑1 (MQ=32) cggcggGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAgc > 3:218835/1‑149 (MQ=32) ggcggGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAgcg > 2:294815/1‑149 (MQ=32) gcggGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAgcg > 4:49957/1‑148 (MQ=32) cTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACggg < 1:169910/149‑1 (MQ=32) tttttGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACggg > 5:151014/1‑148 (MQ=32) tCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATcc < 2:56525/147‑1 (MQ=32) tCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATcc > 1:56525/1‑147 (MQ=32) aaggcgACAAAAAGTAACCAAAAAACGCTGGCTCCTTTCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACgg < 8:37602/135‑1 (MQ=17) aaCGCGACAAAAAGTAACAAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTAGATACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACgg > 7:37602/1‑138 (MQ=12) aCGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGccc < 4:218835/149‑1 (MQ=32) cgACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATGGATGCCCGt > 4:261634/1‑148 (MQ=17) gACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATGGATGCCCGTCa < 3:261634/149‑1 (MQ=17) gACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCa < 3:49957/149‑1 (MQ=32) aCAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTTCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCAc < 5:156082/149‑1 (MQ=17) aCAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATGGATGCCCGTCaa < 1:294815/149‑2 (MQ=17) aaaaGTAACCAAAACACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCTGGGTTCCCTCGCTACGGTCTTTCTCCCGGTAGTACGCGCTGCAGGCCCCATCCATGGGGCCtgcagc < 7:156598/121‑6 (MQ=11) aaaGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTcc < 1:176822/149‑1 (MQ=32) ccTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCAAGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCAGTCACCTCCCTTCGCAAGCCCTGAGCTCGGcc > 8:210190/1‑149 (MQ=255) tcatcCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACTCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTc < 7:83025/148‑1 (MQ=25) atcCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTg < 6:151014/148‑1 (MQ=37) tcCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCCCTAAGCTCATGCTCCCGGGAGTACGCCCTGCAGCCCCCAAACAAGCGGCCT‑CAGCGCTTGAACGGCATCCATCCCAGTCCCCTAACTTCGAAAGCCCTGCGCACGGCCATCTg > 6:180690/1‑147 (MQ=2) tGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATc > 2:103112/1‑149 (MQ=37) gcTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCCGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGc > 5:23243/1‑149 (MQ=37) gcTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGc > 5:27715/1‑149 (MQ=37) tCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGTTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCt > 2:192581/1‑149 (MQ=37) cGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCt < 6:79958/147‑1 (MQ=37) gggTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAAc < 7:60590/149‑1 (MQ=37) gTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACaa > 5:113910/1‑149 (MQ=40) gCTCCCGGGAGTACGCGCTGCAGGCCGCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGc < 5:180690/146‑1 (MQ=255) gCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCaa > 2:203935/1‑148 (MQ=39) gCTCCCGGGAGTACGCGCTGCAGGCCCCAGCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCaa < 4:165988/148‑1 (MQ=255) cTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCaaa < 7:200806/148‑1 (MQ=40) ccGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCg < 4:236260/91‑1 (MQ=33) ccGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCg > 3:236260/1‑91 (MQ=33) ccGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaag > 8:102265/1‑147 (MQ=255) ccGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaag < 7:134266/147‑1 (MQ=255) tcaagcgcTG‑AGGCCCCATGGATGGGGCCTGCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGaa > 8:8100/5‑117 (MQ=11) tcaagcgcTG‑AGGCCCCATGGATGGGGCCTGCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGaa < 7:8100/113‑1 (MQ=11) gcgcTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaagcagaag > 8:132560/1‑149 (MQ=255) cgcTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaagcagaagc < 7:102265/149‑1 (MQ=255) gaGGCCCCATGGATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGc < 3:280091/101‑1 (MQ=255) gaGGCCCCATGGATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGc > 4:280091/2‑102 (MQ=255) gaGGCCCCATGGATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaagcagaagcagaag > 6:38709/2‑149 (MQ=255) cAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCgcgcgc > 6:267964/1‑118 (MQ=39) cAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaagcagaagcaga < 2:252254/147‑1 (MQ=255) cccATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCCCCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTGGCAATCTGCGTCGCCTGAACTACCGCGCGCTTCCAAGCAAAAGCAGAAGCAGAagcagaagccgcc > 8:221417/1‑148 (MQ=255) cccATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaagcagaagcagaagcag > 8:16877/1‑147 (MQ=255) | CCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAAAGCAGAAGCAGAAGCAGAAGCAGCA > NC_002947/4909778‑4910064 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |