Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,930,408 G→C 7.8% L189V (CTG→GTG)  cheZ ← protein phosphatase CheZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,930,4080GC 7.8% 190.7 / 2.9 61L189V (CTG→GTG) cheZprotein phosphatase CheZ
Reads supporting (aligned to +/- strand):  ref base G (26/30);  new base C (3/2);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAG  >  NC_002947/4930265‑4930543
                                                                                                                                               |                                                                                                                                       
cGGACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAgt                                                                                                                                        <  4:161698/145‑1 (MQ=255)
cGGACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTgtgg                                                                                                                                    <  5:194538/149‑1 (MQ=255)
    ccTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGAc                                                                                                                                <  2:41199/149‑1 (MQ=255)
      atccccccGAGTCGTCTGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGAcgc                                                                                                                              <  8:139322/145‑1 (MQ=255)
         aCCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCACACTGGTGAccgagg                                                                                                                           <  3:771/149‑6 (MQ=255)
            ccGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGa                                                                                                                        >  8:28232/1‑149 (MQ=255)
             cGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGa                                                                                                                        >  5:52181/1‑148 (MQ=255)
              gAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGAt                                                                                                                       >  1:114790/1‑148 (MQ=255)
                   ggATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTg                                                                                                                 >  6:247949/1‑149 (MQ=255)
                   ggATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTg                                                                                                                 >  3:184367/1‑149 (MQ=255)
                     aTGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGc                                                                                                               <  5:148356/149‑1 (MQ=255)
                        tttttCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCg                                                                                                             <  7:28232/148‑1 (MQ=255)
                        tttttCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCg                                                                                                             <  4:263716/148‑1 (MQ=255)
                          tttCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGt                                                                                                           <  4:301763/148‑1 (MQ=255)
                               cGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAgg                                                                                                       <  7:58040/147‑1 (MQ=255)
                               cGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAgg                                                                                                       >  8:58040/1‑147 (MQ=255)
                                 aTCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCt                                                                                                   >  7:253000/1‑149 (MQ=255)
                                    attttCAGCACGCAAATGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGa                                                                                                 <  8:71557/147‑1 (MQ=255)
                                     ttttCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTCGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGa                                                                                                 >  7:224351/1‑147 (MQ=255)
                                      tttCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTcc                                                                                                    <  4:184367/143‑1 (MQ=255)
                                      tttCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGa                                                                                                 <  7:218941/146‑1 (MQ=255)
                                      tttCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATa                                                                                               <  6:52181/148‑1 (MQ=255)
                                            cACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCt                                                                                          <  1:73378/147‑1 (MQ=255)
                                            cACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTgg                                                                                        <  1:301104/149‑1 (MQ=255)
                                               gCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAg                                                                                                        <  8:263930/130‑1 (MQ=255)
                                               gCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAg                                                                                                        >  7:263930/1‑130 (MQ=255)
                                                   ttGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGa                                                                                                 <  1:288294/133‑1 (MQ=255)
                                                   ttGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGa                                                                                                 >  2:288294/1‑133 (MQ=255)
                                                          tGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTCAGCAGGTTGCTTTACACCTCGGTCACCAGAGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCATGGGCCAGCAAAATGTc                                                                          >  8:175449/1‑149 (MQ=255)
                                                            gTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAgt                                                                                                                                        >  5:241395/1‑85 (MQ=255)
                                                            gTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAgt                                                                                                                                        <  6:241395/85‑1 (MQ=255)
                                                               aTGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTCGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTg                                                                      <  8:224351/148‑1 (MQ=255)
                                                                tGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAg                                                                    >  3:226794/1‑149 (MQ=255)
                                                                     tataCCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGc                                                                <  5:247949/148‑1 (MQ=255)
                                                                         ccGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTcc                                                                                                    >  5:240611/1‑108 (MQ=255)
                                                                         ccGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTcc                                                                                                    <  6:240611/108‑1 (MQ=255)
                                                                         ccGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCgg                                                            >  7:234106/1‑148 (MQ=255)
                                                                          cGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGa                                                                     <  5:285848/138‑1 (MQ=255)
                                                                          cGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGa                                                                     >  6:285848/1‑138 (MQ=255)
                                                                          cGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGa                                                           >  5:26515/1‑148 (MQ=255)
                                                                           ggCAAAGCGGTCGACCTGGCTGGCGATCAGCACCAGCTTGAGCAGGTTGCTTTCCTCCTCGGTCACCAGTGTGGTGACGCGGTTGATCACGTGGCGGGTCAGGTGCTGATAGTCCTGGGGCATCAAATTGTCGTTGAGGTGCCCGGaaa                                                         <  7:175449/149‑1 (MQ=255)
                                                                               aaGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCtt                                                     <  3:215752/149‑1 (MQ=255)
                                                                                        aCCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTAcc                                            >  8:36601/1‑149 (MQ=255)
                                                                                               tggcCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGgcg                                     >  3:149536/1‑149 (MQ=255)
                                                                                                  ccATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCtg                                  <  8:210143/149‑1 (MQ=255)
                                                                                                   cATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCtgt                                 >  6:36912/1‑149 (MQ=255)
                                                                                                     tCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGc                               <  8:253000/149‑1 (MQ=255)
                                                                                                     tCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGc                               <  5:36912/149‑1 (MQ=255)
                                                                                                     tCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGc                               <  3:234482/149‑1 (MQ=255)
                                                                                                        gCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGt                             >  1:140766/1‑148 (MQ=255)
                                                                                                        gCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGt                             >  1:136096/1‑148 (MQ=255)
                                                                                                         cACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCa                           <  6:26515/149‑1 (MQ=255)
                                                                                                          aCCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAg                          >  2:284988/1‑149 (MQ=255)
                                                                                                                    gCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGc                                                   <  6:25153/114‑1 (MQ=255)
                                                                                                                    gCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGc                                                   >  5:25153/1‑114 (MQ=255)
                                                                                                                       ggTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACt             <  2:114790/149‑1 (MQ=255)
                                                                                                                           gCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCt         >  1:78102/1‑149 (MQ=255)
                                                                                                                                 caagcgcgTCACCACACTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGAc     >  3:3231/8‑147 (MQ=255)
                                                                                                                                  aCCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGAc     >  6:5983/1‑146 (MQ=255)
                                                                                                                                  aCCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAg  <  2:78102/149‑1 (MQ=255)
                                                                                                                                   ccTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAg  >  4:263955/1‑148 (MQ=255)
                                                                                                                                    gcgcgTCACCACACTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCa                           <  7:25391/118‑1 (MQ=255)
                                                                                                                                    gcgcgTCACCACACTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCa                           >  8:25391/5‑122 (MQ=255)
                                                                                                                                    gcgcgTCACCACACTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGAc     >  6:173044/5‑144 (MQ=255)
                                                                                                                                               |                                                                                                                                       
CGGACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAG  >  NC_002947/4930265‑4930543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑