Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 374,742 C→G 8.6% A11G (GCC→GGC)  PP_0312 → electron transfer flavoprotein subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947374,7420CG 8.6% 214.0 / 9.7 70A11G (GCC→GGC) PP_0312electron transfer flavoprotein subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (32/32);  new base G (4/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-01

GCGCCCACAGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGC  >  NC_002947/374597‑374887
                                                                                                                                                  |                                                                                                                                                 
gcgcCCACAGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGcc                                                                                                                                                  >  3:219373/1‑147 (MQ=255)
   cccACAGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTg                                                                                                                                             <  2:282459/149‑1 (MQ=255)
     cacaGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGa                                                                                                                                           <  1:88954/149‑1 (MQ=255)
       caGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATc                                                                                                                                         <  4:219373/149‑1 (MQ=255)
           tCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACTCGGCGCGTGGATCGcgg                                                                                                                                     <  1:167571/149‑3 (MQ=255)
              aGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGt                                                                                                                                   >  3:17412/1‑148 (MQ=255)
                      gCCGAACTTGTGGCCGACGTGCTGATCGAGGAGGACGCGGCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTg                                                                                                                          <  8:108779/149‑1 (MQ=255)
                      gCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTg                                                                                                                          >  3:185729/1‑149 (MQ=255)
                        cGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACTCGGCGCGTGGATCGcgg                                                                                                                                     >  6:206106/1‑134 (MQ=255)
                        cGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACTCGGCGCGTGGATCGcgg                                                                                                                                     <  5:206106/136‑3 (MQ=255)
                        cGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCa                                                                                                                        >  4:242533/1‑149 (MQ=255)
                             tggtggCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCAcc                                                                                                                      <  3:3000/146‑1 (MQ=255)
                             tggtggCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCAcc                                                                                                                      >  4:3000/1‑146 (MQ=255)
                                  gCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCa                                                                                                               <  4:185729/148‑1 (MQ=255)
                                           cTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATg                                                                                                     >  6:204896/1‑149 (MQ=255)
                                              aTCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCAATGATGAGAGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACACGCTGCATGCCGCGATg                                                                                                     >  2:9080/1‑146 (MQ=255)
                                               tCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGc                                                                                                    <  4:178856/146‑1 (MQ=255)
                                               tCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGc                                                                                                    >  3:178856/1‑146 (MQ=255)
                                                       gaCGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCaa                                                                                            >  5:2453/1‑146 (MQ=255)
                                                          gCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATc                                                                                                                                         <  8:133637/98‑1 (MQ=255)
                                                          gCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATc                                                                                                                                         >  7:133637/1‑98 (MQ=255)
                                                          gCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGAc                                                                                       >  3:294049/1‑148 (MQ=255)
                                                            aTCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAg                                                                                    >  3:164279/1‑149 (MQ=255)
                                                            aTCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAg                                                                                    >  1:216193/1‑149 (MQ=255)
                                                                  tGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCTCCCGCGATCCACGCGCCGCGTGCAACGCCCGTAACCGTATGAACCCCCTCCATGCCGCGATGCAGCCGCAAAAGACCAGATGGa                                                                               >  1:246506/1‑149 (MQ=255)
                                                                    tCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATg                                                                             <  4:17412/148‑1 (MQ=255)
                                                                    tCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGGCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATg                                                                             <  1:111887/148‑1 (MQ=255)
                                                                      ccAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATgggg                                                                          >  1:100375/1‑149 (MQ=255)
                                                                       gaaaGCCGCAACCGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGc                                                                         <  8:278774/148‑1 (MQ=255)
                                                                       cAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGc                                                                         >  8:220612/1‑149 (MQ=255)
                                                                        aaaGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGcc                                                                                                                                      >  7:131568/1‑87 (MQ=255)
                                                                        aaaGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGcc                                                                                                                                      <  8:131568/87‑1 (MQ=255)
                                                                        aaaGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGcc                                                                        >  7:218239/1‑149 (MQ=255)
                                                                           gCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCaa                                                                     >  4:230554/1‑149 (MQ=255)
                                                                            ccGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAAc                                                                    <  3:242533/149‑1 (MQ=255)
                                                                                     gCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCt                                                                                  >  1:277854/1‑126 (MQ=255)
                                                                                     gCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCt                                                                                  <  2:277854/126‑1 (MQ=255)
                                                                                     gCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCatcatc                                                           <  5:204896/149‑1 (MQ=255)
                                                                                      cTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATcc                                                          <  8:218239/149‑1 (MQ=255)
                                                                                       tAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGAGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCg                                                         >  4:145892/1‑149 (MQ=255)
                                                                                          aCGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGc                                                        >  8:115815/1‑147 (MQ=255)
                                                                                            gTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCaa                                                                     >  7:134559/1‑132 (MQ=255)
                                                                                            gTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCaa                                                                     <  8:134559/132‑1 (MQ=255)
                                                                                              gAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAAc                                                  >  7:114368/1‑149 (MQ=255)
                                                                                                 ccTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCca                                               <  3:230554/149‑1 (MQ=255)
                                                                                                    gCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACAt                                            >  4:7325/1‑149 (MQ=255)
                                                                                                     cGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACAt                                            <  7:220612/148‑1 (MQ=255)
                                                                                                     cGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACAt                                            <  2:216193/148‑1 (MQ=255)
                                                                                                      ggAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGc                                          <  6:2453/149‑1 (MQ=255)
                                                                                                       gAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGc                                          >  5:125545/1‑148 (MQ=255)
                                                                                                        aGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGa                                        <  4:294049/149‑1 (MQ=255)
                                                                                                          gTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGAtt                                      >  8:242224/1‑149 (MQ=255)
                                                                                                                tgatgaGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCaa                                                                     >  8:173003/1‑112 (MQ=255)
                                                                                                                tgatgaGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCaa                                                                     <  7:173003/112‑1 (MQ=255)
                                                                                                                      gCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCCCCCTCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGGACCCAAATGCGATTGCCGCAGGCttt                          <  1:9080/149‑1 (MQ=255)
                                                                                                                      gCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCttt                          <  3:184111/149‑1 (MQ=255)
                                                                                                                      gCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCttt                          >  4:184111/1‑149 (MQ=255)
                                                                                                                          cATTATCC‑GCCGCGCTCCACGCGCCGTGGGGATCGCCCGTACCCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTcg                      <  4:185763/149‑1 (MQ=255)
                                                                                                                              aTCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTcggcc                   <  8:114368/148‑1 (MQ=255)
                                                                                                                                   gccgcGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATgggg                                                                          <  5:210007/89‑1 (MQ=255)
                                                                                                                                   gccgcGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATgggg                                                                          >  6:210007/1‑89 (MQ=255)
                                                                                                                                   gccgcGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTcggcccggcc              >  7:73405/1‑149 (MQ=255)
                                                                                                                                   gccgcGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTcggcccggcc              <  2:100375/149‑1 (MQ=255)
                                                                                                                                      gcgATCCACTCGGCGCGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGATGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCccggccgg            >  2:232762/1‑148 (MQ=255)
                                                                                                                                      gcgATCCACTCGGCGCGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCccggccgg            >  4:20634/1‑148 (MQ=255)
                                                                                                                                      gcgATCCACTCGGCGCGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCccggccgg            >  8:14987/1‑148 (MQ=255)
                                                                                                                                        gATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCAt         <  7:115815/149‑1 (MQ=255)
                                                                                                                                         aTCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCAt         >  1:155453/1‑148 (MQ=255)
                                                                                                                                            cACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAg     <  2:246506/149‑1 (MQ=255)
                                                                                                                                             aCGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCaacc    >  1:109774/1‑147 (MQ=255)
                                                                                                                                             aCGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAgc    <  3:7325/149‑1 (MQ=255)
                                                                                                                                               gcgcCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAgcgc  <  8:73405/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCGCCCACAGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCC‑GCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGC  >  NC_002947/374597‑374887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑