Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,082,737 A→T 7.5% L510* (TTG→TAG)  alaS ← alanine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,082,7370AT 7.5% 155.7 / 3.2 53L510* (TTG→TAG) alaSalanine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (23/26);  new base T (2/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

GTGGCGTGCTGTACGTCGGCATCGACCTTGGCCTCGACGGGCGAACCGATCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGC  >  NC_002947/5082593‑5082885
                                                                                                                                                |                                                                                                                                                    
gTGGCGTGCTGTACGTCGGCATCGACCTTGGCCTCGACGGGCGAACCGATCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCaagaa                                                                                                                                                  <  4:156222/149‑1 (MQ=255)
     gTGCTGTACGTCGGCATCGACCTTGGCCTCGACGGGCGAACCGATCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCgg                                                                                                                                             <  2:41779/149‑1 (MQ=255)
          gTACGTCGGCATCGACCTTGGCCTCGACGGGCGAACCGATCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACGGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGAGCAGCGCCAGCCTGCAAGAAGCCGGTATca                                                                                                                                        <  1:183520/149‑1 (MQ=255)
          gTACGTCGGCATCGACCTTGGCCTCGACGGGCGAACCGATCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATca                                                                                                                                        >  6:208094/1‑149 (MQ=255)
           tACGTCGGCATCGACCTTGGCCTCGACGGGCGAACCGATCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATcac                                                                                                                                       >  4:100170/1‑149 (MQ=255)
                       gACCTTGGCCTCGACGGGCGAACCGATCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGaccac                                                                                                                           >  5:251014/1‑149 (MQ=255)
                                cTCGACGGGCGAACCGATCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCgg                                                                                                                  <  3:100170/149‑1 (MQ=255)
                                 tCGACGGGCGAACCGATCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCgggtccgcc                                                                                                                 >  3:44752/1‑142 (MQ=255)
                                   gACGGGCGAACCGATCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCg                                                                                                                >  3:244283/1‑148 (MQ=255)
                                                aTCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCTAGAAGCCgg                                                                                                                                             >  8:177929/1‑106 (MQ=255)
                                                aTCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCTAGAAGCCgg                                                                                                                                             >  8:173407/1‑106 (MQ=255)
                                                aTCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCTAGAAGCCgg                                                                                                                                             <  7:177929/106‑1 (MQ=255)
                                                aTCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCTAGAAGCCgg                                                                                                                                             <  7:173407/106‑1 (MQ=255)
                                                     cAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACg                                                                                                         >  4:9956/1‑137 (MQ=255)
                                                     cAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACg                                                                                                         <  3:9956/137‑1 (MQ=255)
                                                     cAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTg                                                                                                    <  2:305567/142‑1 (MQ=255)
                                                     cAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTg                                                                                                    >  1:305567/1‑142 (MQ=255)
                                                      aGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAg                                                                                            >  5:252775/1‑149 (MQ=255)
                                                       gcgcACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGc                                                                                           <  6:221356/149‑1 (MQ=255)
                                                                   tCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGcc                                                                                >  6:187024/1‑148 (MQ=255)
                                                                        acGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCctcctc                                                                          <  8:129776/149‑1 (MQ=255)
                                                                              tggtgCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTc                                                                    <  8:121599/149‑1 (MQ=255)
                                                                                 tgCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCAcc                                                                 <  5:187024/149‑1 (MQ=255)
                                                                                  gCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCAcc                                                                 >  5:82242/1‑148 (MQ=255)
                                                                                    aGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAg                                                              >  1:300327/1‑149 (MQ=255)
                                                                                     ggAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGc                                                             <  2:300327/149‑1 (MQ=255)
                                                                                                 cgGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGAt                                                 <  5:208094/149‑1 (MQ=255)
                                                                                                    tCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTgg                                              <  6:82242/149‑1 (MQ=255)
                                                                                                    tCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTgg                                              <  6:198160/149‑1 (MQ=255)
                                                                                                    tCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTgg                                              >  5:198160/1‑149 (MQ=255)
                                                                                                      ttGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGc                                                                                           >  3:122619/1‑102 (MQ=255)
                                                                                                      ttGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGc                                                                                           <  4:122619/102‑1 (MQ=255)
                                                                                                       tggtggTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTg                                                                                                    <  3:287314/92‑1 (MQ=255)
                                                                                                       tggtggTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTg                                                                                                    >  4:287314/1‑92 (MQ=255)
                                                                                                        ggtggtGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTgg                                              >  5:54168/1‑145 (MQ=255)
                                                                                                        ggtggtGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTgg                                              <  6:54168/145‑1 (MQ=255)
                                                                                                         gtggtgTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTg                                                           >  2:240445/1‑131 (MQ=255)
                                                                                                         gtggtgTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTg                                                           <  1:240445/131‑1 (MQ=255)
                                                                                                            gtgtCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCtt                                      <  7:84273/149‑1 (MQ=255)
                                                                                                            gtgtCGCGCACGGCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCCGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCtt                                      <  8:99904/149‑1 (MQ=255)
                                                                                                            gtggCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCtt                                      <  5:160092/149‑1 (MQ=255)
                                                                                                               tCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACGACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTa                                   >  1:252013/1‑149 (MQ=255)
                                                                                                               tCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGt                                    <  4:244283/148‑1 (MQ=255)
                                                                                                                   gcACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGc                                                                        >  4:56788/1‑108 (MQ=255)
                                                                                                                   gcACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGc                                                                        <  3:56788/108‑1 (MQ=255)
                                                                                                                           aaGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCtt                                      <  3:6252/134‑1 (MQ=255)
                                                                                                                           aaGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCtt                                      >  4:6252/1‑134 (MQ=255)
                                                                                                                            aGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATc                      <  6:251014/149‑1 (MQ=255)
                                                                                                                               gTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTg                   >  5:94438/1‑149 (MQ=255)
                                                                                                                                  cAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGcc                 <  2:130169/148‑1 (MQ=255)
                                                                                                                                  cAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGcc                 >  1:130169/1‑148 (MQ=255)
                                                                                                                                       ccAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGcc           >  7:143726/1‑149 (MQ=255)
                                                                                                                                              gCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTg    >  6:19653/1‑149 (MQ=255)
                                                                                                                                               cAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTg    <  2:252013/148‑1 (MQ=255)
                                                                                                                                                aagaagCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGc  >  6:138546/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GTGGCGTGCTGTACGTCGGCATCGACCTTGGCCTCGACGGGCGAACCGATCACCAGCGCACCGCTGGTCACCACGCCGTGGTGCAGGAAGGCACCGCCGGTCTTGGTGGTGTCGCGCACGTCAAAGCGTGCAGCGCCAGCCTGCAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGC  >  NC_002947/5082593‑5082885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑