Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,096,286	G→T	7.6%	Y160* (TAC→TAA)	argT ←	lysine/arginine/ornithine ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,096,286	0	G	T	7.6%	163.3 / 4.3	61	Y160* (TAC→TAA)	argT	lysine/arginine/ornithine ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (26/30);  new base T (3/2);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01

CTTGGCGTCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGCGCC  >  NC_002947/5096139‑5096433                                                                                                                                                    |                                                                                                                                                     cTTGGCGTCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCg                                                                                                                                                      >  3:4650/1‑148 (MQ=255)  ttGGCGTCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGt                                                                                                                                                     >  5:199948/1‑148 (MQ=255)    ggCGTCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTaa                                                                                                                                                   <  5:107834/148‑1 (MQ=255)    ggCGTCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAg                                                                                                                                                  >  4:49974/1‑149 (MQ=255)       gTCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGca                                                                                                                                               >  8:221951/1‑149 (MQ=255)       gTCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGca                                                                                                                                               >  4:62687/1‑149 (MQ=255)        tCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTATATTTCGTTCTGGGTGCCGTAAGGcac                                                                                                                                              <  6:206645/149‑1 (MQ=255)           gTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGcaccac                                                                                                                                           >  8:151175/1‑149 (MQ=255)             gAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑gg                                                                                                                                        >  3:278176/1‑149 (MQ=255)                   cGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCAc                                                                                                                                  <  7:16949/149‑1 (MQ=255)                        cTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTg                                                                                                                             <  2:282643/149‑1 (MQ=255)                           cGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGgcg                                                                                                                          >  5:225941/1‑149 (MQ=255)                           cGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGgc                                                                                                                           <  6:267717/148‑1 (MQ=255)                              aCGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCTTACGGcacccaga                                                                                                                                         <  2:172329/132‑5 (MQ=255)                              aCGCGAAGCCCTTGCCGGCGTCAGTCTTCAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCTTACGGcacccaga                                                                                                                                         >  1:172329/1‑128 (MQ=255)                               cgcgAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCa                                                                                                                      <  3:62687/149‑1 (MQ=255)                                gcgAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAg                                                                                                                     >  1:897/1‑149 (MQ=255)                                  gAAGCCCTTGCCGGCTTCAGTCTTGAGGAACCCATCTTCCAGCAGGTGGGCGTCAGCCACAGGGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAg                                                                                                                     <  2:97217/147‑1 (MQ=255)                                      cccTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTc                                                                                                               <  7:221951/149‑1 (MQ=255)                                                cGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAg                                                                                                     <  3:49974/149‑1 (MQ=255)                                                      tCTTGCGTAAGCCATGTTCCTGCTGGGTGGCGTCAGCCACAGTGCGGGCGTGGGGGCAAGCTGCAACGTCCAGGTATATTTGGTTCTGGGTGCCGTTAGGCACCAC‑GGTGGCAGCCTTGGCGCCCAGCACTTCCTTGGCGTAGCGGTc                                                                                                <  4:278176/148‑1 (MQ=255)                                                          tcggAAGCAAGATTCCTGCTGGGGGTCGTACGCAACAGGGCCGTCGATGCGGCCCTGTTCAACGTAATGGTTGATTTCGTTCTGGGTGCCTTACGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGt                                                                                              <  2:291107/144‑1 (MQ=255)                                                                gCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGc                                                                                     <  2:897/149‑1 (MQ=255)                                                                  cATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCcg                                                                                   >  8:142470/1‑149 (MQ=255)                                                                  cATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCcg                                                                                   >  6:205731/1‑149 (MQ=255)                                                                   aTCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCcgc                                                                                  >  3:65064/1‑149 (MQ=255)                                                                               gggggCGTCAGCAACAGTGCCGTCGCGGCGGCAAGCTGCAACGTCCAGTTACTTTTCGTTCTGGGGGCGGAACGCCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGa                                                                      <  6:199948/146‑1 (MQ=255)                                                                                   gCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTTGTGAATGGAGCCGCGCTGCACGCCGAtctt                                                                  <  6:111190/149‑1 (MQ=255)                                                                                   gCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTg                                                                              <  1:188056/137‑1 (MQ=255)                                                                                   gCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTg                                                                              >  2:188056/1‑137 (MQ=255)                                                                                    cGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGAtcttc                                                                 <  5:236758/149‑1 (MQ=255)                                                                                     gTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGAtcttct                                                                >  7:188588/1‑149 (MQ=255)                                                                                      tCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑AGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATcttctt                                                               >  2:304336/1‑149 (MQ=255)                                                                                          ccACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCt                                                           >  2:188305/1‑149 (MQ=255)                                                                                          ccACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCt                                                           <  7:142470/149‑1 (MQ=255)                                                                                           cacaGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGGGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCtt                                                          <  6:225941/149‑1 (MQ=255)                                                                                            acaGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCttt                                                         <  1:188305/149‑1 (MQ=255)                                                                                            acaGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCatt                                                         <  4:65064/149‑1 (MQ=255)                                                                                            acaGGGCCGGCGTGGCGGCCAGCTGCACCGTCCAGGTATATTTCGTGCTGGGTGCGGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCttt                                                         <  4:288586/149‑1 (MQ=255)                                                                                                        cggcgccCAGCTGCAAGGGCCCGGTAGTTTGCGTTCGGGGGGCGGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAg                                             <  4:4650/148‑1 (MQ=255)                                                                                                         ggcggcCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCACGGGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAg                                             >  1:238501/1‑149 (MQ=255)                                                                                                                 gCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑AGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCt                                    <  1:304336/149‑1 (MQ=255)                                                                                                                    gCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGc                                                             <  8:65512/121‑1 (MQ=255)                                                                                                                    gCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGc                                                             >  7:65512/1‑121 (MQ=255)                                                                                                                     cAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCa                                                       <  1:197576/126‑1 (MQ=255)                                                                                                                     cAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCa                                                       >  2:197576/1‑126 (MQ=255)                                                                                                                     cAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACg                                >  5:49450/1‑149 (MQ=255)                                                                                                                     cAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACg                                >  4:91187/1‑149 (MQ=255)                                                                                                                       aCGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACggt                              >  5:91596/1‑149 (MQ=255)                                                                                                                         gTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACggtgg                            >  7:71233/1‑149 (MQ=255)                                                                                                                               gTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGt                      >  3:107017/1‑149 (MQ=255)                                                                                                                                    tttCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCttt                                                         <  2:45058/109‑1 (MQ=255)                                                                                                                                    tttCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCttt                                                         >  1:45058/1‑109 (MQ=255)                                                                                                                                         ttCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGAc            >  8:154243/1‑149 (MQ=255)                                                                                                                                           cgtggTGCCTTACGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGAc            >  5:181171/4‑147 (MQ=255)                                                                                                                                           cgtggTGCCTTACGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGAc            >  8:16871/4‑147 (MQ=255)                                                                                                                                              ggTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAgg        <  2:238501/148‑1 (MQ=255)                                                                                                                                              ggTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAg         <  8:188588/147‑1 (MQ=255)                                                                                                                                                  ccGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTcc                    <  6:225671/132‑1 (MQ=255)                                                                                                                                                  ccGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTcc                    >  5:225671/1‑132 (MQ=255)                                                                                                                                                  ccGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGgcgcgc   <  8:71233/149‑1 (MQ=255)                                                                                                                                                   cGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGCGcc  <  3:91187/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CTTGGCGTCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGCGCC  >  NC_002947/5096139‑5096433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑