Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,096,289	A→C	9.7%	P159P (CCT→CCG)	argT ←	lysine/arginine/ornithine ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,096,289	0	A	C	9.7%	149.8 / 6.3	59	P159P (CCT→CCG)	argT	lysine/arginine/ornithine ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (25/28);  new base C (3/3);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

GGCGTCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGCGCCGGAG  >  NC_002947/5096142‑5096437                                                                                                                                                    |                                                                                                                                                      ggCGTCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTaa                                                                                                                                                       <  5:107834/148‑1 (MQ=255) ggCGTCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAg                                                                                                                                                      >  4:49974/1‑149 (MQ=255)    gTCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGca                                                                                                                                                   >  8:221951/1‑149 (MQ=255)    gTCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGca                                                                                                                                                   >  4:62687/1‑149 (MQ=255)     tCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTATATTTCGTTCTGGGTGCCGTAAGGcac                                                                                                                                                  <  6:206645/149‑1 (MQ=255)        gTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGcaccac                                                                                                                                               >  8:151175/1‑149 (MQ=255)          gAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑gg                                                                                                                                            >  3:278176/1‑149 (MQ=255)                cGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCAc                                                                                                                                      <  7:16949/149‑1 (MQ=255)                     cTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTg                                                                                                                                 <  2:282643/149‑1 (MQ=255)                        cGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGgcg                                                                                                                              >  5:225941/1‑149 (MQ=255)                        cGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGgc                                                                                                                               <  6:267717/148‑1 (MQ=255)                           aCGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCTTACGGcacccaga                                                                                                                                             <  2:172329/132‑5 (MQ=255)                           aCGCGAAGCCCTTGCCGGCGTCAGTCTTCAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCTTACGGcacccaga                                                                                                                                             >  1:172329/1‑128 (MQ=255)                            cgcgAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCa                                                                                                                          <  3:62687/149‑1 (MQ=255)                             gcgAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAg                                                                                                                         >  1:897/1‑149 (MQ=255)                               gAAGCCCTTGCCGGCTTCAGTCTTGAGGAACCCATCTTCCAGCAGGTGGGCGTCAGCCACAGGGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAg                                                                                                                         <  2:97217/147‑1 (MQ=255)                                   cccTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTc                                                                                                                   <  7:221951/149‑1 (MQ=255)                                             cGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAg                                                                                                         <  3:49974/149‑1 (MQ=255)                                                   tCTTGCGTAAGCCATGTTCCTGCTGGGTGGCGTCAGCCACAGTGCGGGCGTGGGGGCAAGCTGCAACGTCCAGGTATATTTGGTTCTGGGTGCCGTTAGGCACCAC‑GGTGGCAGCCTTGGCGCCCAGCACTTCCTTGGCGTAGCGGTc                                                                                                    <  4:278176/148‑1 (MQ=255)                                                       tcggAAGCAAGATTCCTGCTGGGGGTCGTACGCAACAGGGCCGTCGATGCGGCCCTGTTCAACGTAATGGTTGATTTCGTTCTGGGTGCCTTACGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGt                                                                                                  <  2:291107/144‑1 (MQ=255)                                                             gCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGc                                                                                         <  2:897/149‑1 (MQ=255)                                                               cATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCcg                                                                                       >  6:205731/1‑149 (MQ=255)                                                               cATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCcg                                                                                       >  8:142470/1‑149 (MQ=255)                                                                aTCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCcgc                                                                                      >  3:65064/1‑149 (MQ=255)                                                                            gggggCGTCAGCAACAGTGCCGTCGCGGCGGCAAGCTGCAACGTCCAGTTACTTTTCGTTCTGGGGGCGGAACGCCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGa                                                                          <  6:199948/146‑1 (MQ=255)                                                                                gCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTTGTGAATGGAGCCGCGCTGCACGCCGAtctt                                                                      <  6:111190/149‑1 (MQ=255)                                                                                gCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTg                                                                                  <  1:188056/137‑1 (MQ=255)                                                                                gCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTg                                                                                  >  2:188056/1‑137 (MQ=255)                                                                                 cGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGAtcttc                                                                     <  5:236758/149‑1 (MQ=255)                                                                                  gTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGAtcttct                                                                    >  7:188588/1‑149 (MQ=255)                                                                                   tCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑AGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATcttctt                                                                   >  2:304336/1‑149 (MQ=255)                                                                                       ccACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCt                                                               >  2:188305/1‑149 (MQ=255)                                                                                       ccACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCt                                                               <  7:142470/149‑1 (MQ=255)                                                                                        cacaGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGGGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCtt                                                              <  6:225941/149‑1 (MQ=255)                                                                                         acaGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCttt                                                             <  1:188305/149‑1 (MQ=255)                                                                                         acaGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCatt                                                             <  4:65064/149‑1 (MQ=255)                                                                                         acaGGGCCGGCGTGGCGGCCAGCTGCACCGTCCAGGTATATTTCGTGCTGGGTGCGGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCttt                                                             <  4:288586/149‑1 (MQ=255)                                                                                                     cggcgccCAGCTGCAAGGGCCCGGTAGTTTGCGTTCGGGGGGCGGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAg                                                 <  4:4650/148‑1 (MQ=255)                                                                                                      ggcggcCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCACGGGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAg                                                 >  1:238501/1‑149 (MQ=255)                                                                                                              gCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑AGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCt                                        <  1:304336/149‑1 (MQ=255)                                                                                                                 gCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGc                                                                 >  7:65512/1‑121 (MQ=255)                                                                                                                 gCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGc                                                                 <  8:65512/121‑1 (MQ=255)                                                                                                                  cAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCa                                                           >  2:197576/1‑126 (MQ=255)                                                                                                                  cAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCa                                                           <  1:197576/126‑1 (MQ=255)                                                                                                                  cAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACg                                    >  5:49450/1‑149 (MQ=255)                                                                                                                  cAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACg                                    >  4:91187/1‑149 (MQ=255)                                                                                                                    aCGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACggt                                  >  5:91596/1‑149 (MQ=255)                                                                                                                      gTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACggtgg                                >  7:71233/1‑149 (MQ=255)                                                                                                                            gTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGt                          >  3:107017/1‑149 (MQ=255)                                                                                                                                 tttCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCttt                                                             >  1:45058/1‑109 (MQ=255)                                                                                                                                 tttCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCttt                                                             <  2:45058/109‑1 (MQ=255)                                                                                                                                      ttCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGAc                >  8:154243/1‑149 (MQ=255)                                                                                                                                        cgtggTGCCTTACGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGAc                >  5:181171/4‑147 (MQ=255)                                                                                                                                        cgtggTGCCTTACGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGAc                >  8:16871/4‑147 (MQ=255)                                                                                                                                           ggTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAgg            <  2:238501/148‑1 (MQ=255)                                                                                                                                           ggTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAg             <  8:188588/147‑1 (MQ=255)                                                                                                                                               ccGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTcc                        <  6:225671/132‑1 (MQ=255)                                                                                                                                               ccGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTcc                        >  5:225671/1‑132 (MQ=255)                                                                                                                                               ccGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGgcgcgc       <  8:71233/149‑1 (MQ=255)                                                                                                                                                cGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGCGcc      <  3:91187/149‑1 (MQ=255)                                                                                                                                                    aGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGCGcc      <  4:139846/145‑1 (MQ=255)                                                                                                                                                    aGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGCGCCgag   >  3:139846/1‑146 (MQ=255)                                                                                                                                                    aGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGCGCCGGAg  >  1:271724/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                      GGCGTCGGTGAAGGCCGGGCCTACGAACGCGAAGCCCTTGCCGGCGTCAGTCTTGAGGAAGCCATCTTCCAGCAGGGTGGCGTCAGCCACAGTGCCGTCGAGGCGGCCAGCTGCAACGTCCAGGTAGATTTCGTTCTGGGTGCCGTAAGGCACCAC‑GGTGGCACCCTTGGCGCCCAGCACTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGCGCCGGAG  >  NC_002947/5096142‑5096437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑