Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,097,145 T→G 7.8% intergenic (+136/+156) PP_mr66 → / ← acsA‑I P6/acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,097,1450TG 7.8% 150.1 / 4.7 51intergenic (+136/+156)PP_mr66/acsA‑IP6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (24/23);  new base G (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

GAAATAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGT  >  NC_002947/5097001‑5097285
                                                                                                                                                |                                                                                                                                             
gAAATAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGc                                                                                                                                           >  6:194406/1‑149 (MQ=255)
       aaCCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCg                                                                                                                                    <  1:45596/149‑1 (MQ=255)
       aaCCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACCACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGCACAGGTTCGGGGcgc                                                                                                                                    <  2:19606/149‑3 (MQ=255)
          cTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTg                                                                                                                                 <  8:38979/149‑1 (MQ=255)
            ataGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGCACAGGTTCGGGGc                                                                                                                                      <  7:18820/142‑1 (MQ=255)
            ataGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGCACAGGTTCGGGGc                                                                                                                                      >  8:18820/1‑142 (MQ=255)
             taGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACa                                                                                                                              <  3:103819/149‑1 (MQ=255)
              aGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGa                                                                                                                                >  8:220133/1‑146 (MQ=255)
               gACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAg                                                                                                                             >  4:102521/1‑148 (MQ=255)
                aCCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGaa                                                                                                                           >  1:279350/1‑149 (MQ=255)
                    aGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGACGGAATTGTTTCATGGCGCCCCGAACCTGTGCGGGGCCTGTGACAGAACAg                                                                                                                        >  1:298442/1‑148 (MQ=255)
                     gCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGt                                                                                                                       >  1:158636/1‑148 (MQ=255)
                       aaTTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAAc                                                                                                                    <  4:203686/149‑1 (MQ=255)
                       aaTTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAAc                                                                                                                    <  4:207734/149‑1 (MQ=255)
                           ccTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCga                                                                                                                 <  2:158636/148‑1 (MQ=255)
                                gCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTg                                                                                                           >  2:40821/1‑149 (MQ=255)
                                   tattatTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCg                                                                                                        >  1:48980/1‑149 (MQ=255)
                                    attattGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCgg                                                                                                       >  4:126205/1‑149 (MQ=255)
                                     ttattGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCggg                                                                                                      >  6:55933/1‑149 (MQ=255)
                                     ttattGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCgg                                                                                                       <  4:181337/148‑1 (MQ=255)
                                          ggCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAAc                                                                                                  <  3:60061/148‑1 (MQ=255)
                                          ggCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGGGACAGAACAGTAACCGATGATTGTCGGGTAAc                                                                                                  <  3:102521/148‑1 (MQ=255)
                                           gCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCATGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCAGGCGCCCCGCACAGGTTCGGGGc                                                                                                                                      >  7:195044/1‑111 (MQ=255)
                                            cAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAAt                                                                                               >  2:167424/1‑149 (MQ=255)
                                                        cgtcgGCAAGGGCATCGTGCCTTGATTCACAAAATTCTGACTCCGATGCACCGAAACGGGGCGGAATTGTTTCCTGTCGCCCCGAAACTTTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGTGc                                                                                  <  2:298442/146‑1 (MQ=255)
                                                        cgacgGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGc                                                                                  >  8:60365/1‑149 (MQ=255)
                                                         gacgGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGAAA‑AAGCGGGCt                                                                                 >  1:202463/1‑149 (MQ=255)
                                                            ggCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGc                                                                              <  2:279350/149‑1 (MQ=255)
                                                                aGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCcgc                                                                          <  7:220133/149‑1 (MQ=255)
                                                                  tGCAGCGTGCCTTGATGCACAAAATCCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGTTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTCGCCCGCGa                                                                        <  5:194062/149‑1 (MQ=255)
                                                                           ccTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCTCGCGAATGCGTGa                                                                <  5:194406/148‑1 (MQ=255)
                                                                            cTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGt                                                                  <  4:147966/145‑1 (MQ=255)
                                                                            cTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGt                                                                  >  3:147966/1‑145 (MQ=255)
                                                                            cTTGATGCACAAAATGCTGACTCCGATGAACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAgg                                                              >  7:221163/1‑149 (MQ=255)
                                                                                   cacaAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAACAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAggg                                                        <  1:40821/149‑1 (MQ=255)
                                                                                   cacaAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAGGTCAggg                                                        >  7:43916/1‑148 (MQ=255)
                                                                                   cacaAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAGGTCAggg                                                        <  5:55933/148‑1 (MQ=255)
                                                                                        aaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGc                                                  >  8:138953/1‑149 (MQ=255)
                                                                                          tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGACCGCGAATGCGTTAGGTCAGGGGACAGCt                                                 >  6:79620/1‑148 (MQ=255)
                                                                                             cgccgacGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGc                                             <  4:138581/143‑1 (MQ=255)
                                                                                               aCTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCCAATGGGTGAGGTCAGGGGACAGCTTAGCCt                                           >  3:276955/1‑149 (MQ=255)
                                                                                                   cGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGaa                                        <  2:48980/148‑1 (MQ=255)
                                                                                                     aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTg                                     <  3:126205/149‑1 (MQ=255)
                                                                                                        cACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGAc                                   <  8:43916/148‑1 (MQ=255)
                                                                                                        cACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGAc                                   <  1:167424/148‑1 (MQ=255)
                                                                                                           cGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGGCAGCTTAGCCTGAACTGACGCAt                               >  5:64100/1‑149 (MQ=255)
                                                                                                             aaaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCGGAACTGACGCATTc                             >  3:53161/1‑149 (MQ=255)
                                                                                                                   gCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTCGCGGGc                       <  7:60365/149‑1 (MQ=255)
                                                                                                                     cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTTCCCGCGCATTGGGGAGGTGAGGGGGCAGCGTAGGCGGAACTGAAGCCATAGCgggg                       >  5:23855/1‑146 (MQ=255)
                                                                                                                                        cccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGCCAGCTTAGCCTGAACTGACGCAATAGCGGGCAAGCCCGCGCCGGCAGGGGGt  >  8:267509/1‑149 (MQ=255)
                                                                                                                                         ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTTCCCCCGAATGCCTGAAGTCATGGGACAGCATATCCTCAAATGACGCATAAGCCGGCAACCCCCCGCCTGCAgggg    >  3:300103/1‑146 (MQ=255)
                                                                                                                                                |                                                                                                                                             
GAAATAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAA‑AAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGT  >  NC_002947/5097001‑5097285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑