Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 378,809 C→T 8.9% K202K (AAG→AAA)  gbcA ← glycine‑betaine dioxygenase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947378,8090CT 8.9% 124.1 / 2.9 45K202K (AAG→AAA) gbcAglycine‑betaine dioxygenase subunit
Reads supporting (aligned to +/- strand):  ref base C (20/21);  new base T (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

GCGGCCACATGGTCCTTGAATTCCTGGCTGGCGCGCGGGTCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAGGAACTCGTCGATGGCAGGCGGGTTTTCCGCCAGGCTGATGAAGATGTAGCCGCCGGCCACCTTCACGT  >  NC_002947/378666‑378957
                                                                                                                                               |                                                                                                                                                    
gcggcCACATGGTCCTTGAATTCCTGGCTGGCGCGCGGGTCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCtttt                                                                                                                                                  <  5:153763/148‑1 (MQ=255)
gcggcCACATGGTCCTTGAATTCCTGGCTGGCGCGCGGGTCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCtttt                                                                                                                                                  >  6:153763/1‑148 (MQ=255)
 cggcCACAAGCTCCTTGAATTCCTGGCCGGCGCGCGGGTCGTTGGTGGCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCCCTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTcc                                                                                                                                                <  8:235203/149‑1 (MQ=255)
   gcCACATGGTCCTTGAATTCCTGGCTGGCGCGCGGGTCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCa                                                                                                                                               >  4:222562/1‑148 (MQ=255)
       caTGGTCCTTGAATTCCTGGCTGGCGCGCGGGTCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAg                                                                                                                                          <  3:222562/149‑1 (MQ=255)
              cTTGAATTCCTGGCTGGCGCGCGGGTCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGttt                                                                                                                                   >  6:254827/1‑149 (MQ=255)
              cTTGAATTCCTGGCTGGCGCGCGGGTCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGttt                                                                                                                                   <  5:254827/149‑1 (MQ=255)
                    ttCCTGGCTGGCGCGCGGGTCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCg                                                                                                                             >  7:6328/1‑149 (MQ=255)
                       ctggctggCGCGCGGGTCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTg                                                                                                                                  <  6:256240/141‑1 (MQ=255)
                       ctggctggCGCGCGGGTCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTg                                                                                                                                  >  5:256240/1‑141 (MQ=255)
                            tggcGCGCGGGTCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAATGTAGTTTGCACCGCCACCTTg                                                                                                                     <  6:70544/149‑1 (MQ=255)
                              gcgcgcGGGTCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGgt                                                                                                                   <  8:213305/149‑1 (MQ=255)
                                  gcgGGGCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTtc                                                                                                               <  6:61301/149‑1 (MQ=255)
                                   cgGGTCGTTGGTGTCGTCCCACTCCAGCAGGGTTGGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTtct                                                                                                              <  6:139323/149‑1 (MQ=255)
                                      gTCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCa                                                                                                           >  3:259926/1‑149 (MQ=255)
                                              tgtCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGtt                                                                                                                >  6:104316/1‑136 (MQ=255)
                                              tgtCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGtt                                                                                                                <  5:104316/136‑1 (MQ=255)
                                              tgtCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTAc                                                                                                   >  2:3388/1‑149 (MQ=255)
                                               gtcgtcCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTc                                                                                                                                                 <  1:42374/102‑1 (MQ=255)
                                               gtcgtcCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTc                                                                                                                                                 >  2:42374/1‑102 (MQ=255)
                                                  gtcCCTCTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGCTAGCACTCGCGGTTGTTTTCCAGCACCAGATTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTTGTGTTCTCCATGTCGTACGGtt                                                                                               <  8:81201/149‑1 (MQ=255)
                                                  gtcCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGtt                                                                                               >  3:92324/1‑149 (MQ=255)
                                                    cccACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCTTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTc                                                                                              >  4:115416/1‑148 (MQ=255)
                                                    cccACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCTTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTc                                                                                              >  4:110836/1‑148 (MQ=255)
                                                    cccACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTc                                                                                              >  2:82265/1‑148 (MQ=255)
                                                     ccACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTcc                                                                                             <  4:92324/148‑1 (MQ=255)
                                                                 ggTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCAATGTCGTACGGTTCCATGTAATGGTc                                                                                  >  7:289804/1‑147 (MQ=255)
                                                                   tttGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCTTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAgg                                                                              <  3:115416/149‑1 (MQ=255)
                                                                   tttGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCTTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAgg                                                                              <  3:110836/149‑1 (MQ=255)
                                                                     tGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAggg                                                                             <  1:3388/148‑1 (MQ=255)
                                                                        agcagTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGc                                                                         >  4:151310/1‑149 (MQ=255)
                                                                         gcagTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGcc                                                                        <  8:6328/149‑1 (MQ=255)
                                                                             ttCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAgg                                                                     <  2:30303/148‑1 (MQ=255)
                                                                                          ccGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAGGAACTCGTCGAt                                                          >  7:224046/1‑146 (MQ=255)
                                                                                          ccGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAGGAACTCGTCGAt                                                          <  8:224046/146‑1 (MQ=255)
                                                                                                       aGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAGGAACTCGTCGATGGCAGGCGGGTTTTcc                                          >  6:274503/1‑149 (MQ=255)
                                                                                                           cTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAGGAACTCGTCGATGGCAGGCGGGTTTTCCGCCa                                      <  5:274503/149‑1 (MQ=255)
                                                                                                                       ttCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAGGAACTCGTCGATGGCAGGCGGGTTTTCCGCCAGGCTGATGAAg                           <  2:246360/148‑1 (MQ=255)
                                                                                                                          cAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAGGAACTCGTCGATGGCAGGCGGGTTTTCCGCCAGGCTGATGAAGATGt                       <  1:82265/149‑1 (MQ=255)
                                                                                                                                cAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAGGAACTCGTCGATGGCAGGCGGGTTTTCCGCCAGGCTGATGAAGATGTAgccgc                 >  3:269602/1‑149 (MQ=255)
                                                                                                                                       cAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAGGAACTCGTCGATGGCAGGCGGGTTTTCCGCCAGGCTGATGAAGATGTAGCCGCCGGCCAc          >  8:202125/1‑149 (MQ=255)
                                                                                                                                        aGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTGTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGAGGCCAGGATCTCGTCGATGGCAGGCGGGTTTTCCGCCAGGCTGATGAAGATCTAGCCGACGGCCAcc         >  8:178705/1‑149 (MQ=255)
                                                                                                                                        aGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAGGAACTCGTCGATGGCAGGCGGGTTTTCCGCCAGGCTCATGAAGATGTAGCCGCCGGCCAcc         >  8:174146/1‑149 (MQ=255)
                                                                                                                                           tGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAGGAACTCGTCGATGGCAGGCGGGTTTTCCGCCAGGCTGATGAAGATGTAGCCGCCGGCCACCtt       >  4:239359/1‑148 (MQ=255)
                                                                                                                                             gCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAGGAACTCGTCGATGGCAGGCGGGTTTTCCGCCAGGCTGATGAAGATGTAGCCGCCGGCCACCttcac    <  4:259926/149‑1 (MQ=255)
                                                                                                                                               cTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAGGAACTCGTCGATGGCAGGCGGGTTTTCCGCCAGGCTGATGAAGATGTAGCCGACGGCCACCttcacgt  >  6:172387/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
GCGGCCACATGGTCCTTGAATTCCTGGCTGGCGCGCGGGTCGTTGGTGTCGTCCCACTCCAGCAGGGTTTGCAGCAGTTCCGGGTGCGAACCGTTGCAGTGGTAGCACTCGCGGTTGTTTTCCAGCACCAGTTTCCAGTTGGCCTTTTCCATCAAGGTAGTTTGCACCGCCACCTTGGTGTTCTCCATGTCGTACGGTTCCATGTAATGGTCCAGGGTGGCCAGGAACTCGTCGATGGCAGGCGGGTTTTCCGCCAGGCTGATGAAGATGTAGCCGCCGGCCACCTTCACGT  >  NC_002947/378666‑378957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑