Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,139,337 G→C 9.1% V143V (GTG→GTC)  PP_4524 → sodium‑solute symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,139,3370GC 9.1% 157.4 / 5.1 53V143V (GTG→GTC) PP_4524sodium‑solute symporter
Reads supporting (aligned to +/- strand):  ref base G (22/26);  new base C (2/3);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTGATGTTCATC  >  NC_002947/5139197‑5139478
                                                                                                                                            |                                                                                                                                              
ctgcgcATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGa                                                                                                                                        <  4:71673/149‑1 (MQ=255)
   cgcATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCAGCATGCTGGCTTATGCACTGATGATCGTGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCt                                                                                                                                     <  5:213255/149‑1 (MQ=255)
      aTCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTCCAGCAGCACCTGca                                                                                                                                  <  4:48942/149‑2 (MQ=255)
           cACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTCCAGCAGCACCTGcag                                                                                                                                 <  3:61686/145‑3 (MQ=255)
            aCCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCt                                                                                                                             <  1:4918/148‑1 (MQ=255)
                tGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCt                                                                                                                        >  4:171319/1‑149 (MQ=255)
                    ccAGGTCCTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTtctc                                                                                                                    >  2:44505/1‑149 (MQ=255)
                    ccAGGTACTGGCGCAACGCTACCACCCCCCCGCACGCCAGGCCAGCGCCGTCATCAGGCTGGCTTATGCACTGATGATCTGGGTCCCCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCGGCTGGACCTGCCGTTCTGGGCTtctc                                                                                                                    <  8:77165/149‑1 (MQ=255)
                    ccAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTtctc                                                                                                                    >  3:156881/1‑149 (MQ=255)
                      aGGTACTGGAGCAACGCTACAACCCCACAGCACGCCAGGCCAGCGCCGTCATCATGATGGCTTATGCACTGATGATCGGGGTAACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTCCTGCTGGACCTGCCGTTCTGGGCTtctat                                                                                                                   >  7:264445/1‑146 (MQ=255)
                         tgcTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATg                                                                                                               <  1:44505/147‑1 (MQ=255)
                           cTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGc                                                                                                              <  1:299347/148‑1 (MQ=255)
                              gAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTcgg                                                                                                          >  8:228751/1‑149 (MQ=255)
                              gAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTcgg                                                                                                          >  3:202268/1‑149 (MQ=255)
                                 cAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTcggcg‑g                                                                                                      <  4:156881/149‑1 (MQ=255)
                                 cAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTcggcg‑g                                                                                                      >  1:212546/1‑149 (MQ=255)
                                       tACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCt                                                                                                                             >  7:66725/1‑121 (MQ=255)
                                       tACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGt                                                                                                >  1:210175/1‑149 (MQ=255)
                                         cAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTg                                                                                                                  <  2:221330/130‑1 (MQ=255)
                                         cAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTg                                                                                                                  >  1:221330/1‑130 (MQ=255)
                                               cACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGc                                                                                        >  6:274616/1‑149 (MQ=255)
                                                aCCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCt                                                                                       <  2:212546/149‑1 (MQ=255)
                                                            gccagCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCAt                                                                           >  2:186414/1‑149 (MQ=255)
                                                              cagCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATcg                                                                         <  4:202268/149‑1 (MQ=255)
                                                                gcgcCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATcggc                                                                       <  3:171319/149‑1 (MQ=255)
                                                                    cGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCa                                                                   >  4:99657/1‑149 (MQ=255)
                                                                     gTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCAt                                                                  <  5:274616/149‑1 (MQ=255)
                                                                        atcatGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGAAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCCGCAtgtg                                                               >  4:44186/1‑149 (MQ=255)
                                                                           atGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCATGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTc                                                            <  8:23635/149‑1 (MQ=255)
                                                                           atGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCGCGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGt                                                             <  1:58430/149‑1 (MQ=255)
                                                                           atGCTGGCTTATGCACTCATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCCGCAAGTGGt                                                             >  2:58430/1‑148 (MQ=255)
                                                                                 ggttctGCACTGATGATCGGTGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTTGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAAc                                                      <  8:264445/144‑1 (MQ=255)
                                                                                  cTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAAcc                                                     <  1:186414/149‑1 (MQ=255)
                                                                                     aTGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCt                                                   >  8:57243/1‑148 (MQ=255)
                                                                                         aCTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGAcc                                               >  3:256880/1‑148 (MQ=255)
                                                                                         aCTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGAcc                                               >  2:39316/1‑148 (MQ=255)
                                                                                           tgatgaTCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAAcc                                                     >  4:17849/1‑140 (MQ=255)
                                                                                           tgatgaTCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAAcc                                                     <  3:17849/140‑1 (MQ=255)
                                                                                               gatCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATc                                         <  7:228751/148‑1 (MQ=255)
                                                                                                atCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGt                                       >  3:149310/1‑149 (MQ=255)
                                                                                                 tCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTGCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTc                                      <  1:211943/149‑1 (MQ=255)
                                                                                                              gACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCa                         <  7:57243/149‑1 (MQ=255)
                                                                                                                 ccTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGa                      >  5:53476/1‑149 (MQ=255)
                                                                                                                 ccTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGa                      >  6:183646/1‑149 (MQ=255)
                                                                                                                       catggccaCCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCg                <  1:39316/149‑1 (MQ=255)
                                                                                                                        atggccaCCGTCCTGCAGGTGCTGGTGGACCTGCCGTTCGGGGCGTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGGTAACCCTGCCCGATATCGTCGAGTTCGTGATCAAGACCGTCgg               >  4:29110/1‑149 (MQ=255)
                                                                                                                            ccaCCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTg           <  1:196069/149‑1 (MQ=255)
                                                                                                                             caCCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTGa          <  4:149310/149‑1 (MQ=255)
                                                                                                                              aCCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTGAt         <  5:183646/149‑1 (MQ=255)
                                                                                                                                    cggCAGGTCCAGCAGCACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTGATGtt      <  6:193173/144‑1 (MQ=255)
                                                                                                                                    cggCAGGTCCAGCAGCACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTGATGtt      >  5:193173/3‑146 (MQ=255)
                                                                                                                                       cAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTGATGTTCATc  <  3:99657/147‑1 (MQ=255)
                                                                                                                                       cAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTGATGTTCATc  <  2:210175/147‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                              
CTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCG‑GCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTGATGTTCATC  >  NC_002947/5139197‑5139478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑