| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,181,152 | A→T | 10.2% | L264Q (CTG→CAG) | PP_4560 ← | ribonuclease BN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,181,152 | 0 | A | T | 10.2% | 130.6 / 6.1 | 49 | L264Q (CTG→CAG) | PP_4560 | ribonuclease BN |
| Reads supporting (aligned to +/- strand): ref base A (23/21); new base T (2/3); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01 | |||||||||||
TATGAGGCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGCCAGCACCGAGCC > NC_002947/5181006‑5181300 | tATGAGGCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGagcagcag > 8:57022/1‑148 (MQ=255) gCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACgg > 7:71195/1‑147 (MQ=255) ttGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGCTGCCGTGCTGCTGCTCGGcgc < 4:241789/149‑3 (MQ=255) ggggTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTCCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGaaa < 2:269255/148‑1 (MQ=255) gggTTTGGGGGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGaaa < 8:167459/148‑1 (MQ=255) tttGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGCTGCCGTGCTGCTGCTCGGC‑GCCGAAatgaa < 6:183091/149‑4 (MQ=38) tttGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAAtata < 5:45333/149‑1 (MQ=255) gggTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGa < 1:160793/148‑1 (MQ=255) ggTGCTCGTGACCCAGTACAGTCAACGTATCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGaa < 5:249118/148‑1 (MQ=255) tGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAAt > 5:186709/1‑148 (MQ=255) cccAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATacagcaacag > 5:44787/1‑149 (MQ=255) ccAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATacagcaacag > 4:167812/1‑148 (MQ=255) cAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGAtt < 7:57022/148‑1 (MQ=255) aGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCa > 8:219316/1‑149 (MQ=255) ggTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTGGATCACCGCATTCATTTCGGC‑GCAGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCAc > 5:114727/1‑149 (MQ=255) cccGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATg < 6:186709/148‑1 (MQ=255) ccGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGAtt < 5:15320/126‑1 (MQ=255) ccGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGAtt > 6:15320/1‑126 (MQ=255) ccGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGc > 1:169086/1‑149 (MQ=255) gAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGCTGCCGTGCTGCTGCTCGGC‑GCCGaaa < 3:7191/92‑1 (MQ=25) gAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGCTGCCGTGCTGCTGCTCGGC‑GCCGaaa > 4:7191/1‑92 (MQ=25) tCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTc < 7:219316/149‑1 (MQ=255) tttCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATTCTGCCGTACATGGCGTTGTAGTCg > 3:38003/1‑148 (MQ=255) tctcGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCgg < 2:301670/147‑1 (MQ=255) tcGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACAt < 2:185643/131‑1 (MQ=255) tcGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACAt > 1:185643/1‑131 (MQ=255) gCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGGTGCCGTAGATGGCGTGGTAGTCGGCTAAgg > 4:26224/1‑149 (MQ=255) gCCCGGGTTCTTGCCTTCGGCTGACAGGTGCTCGCTCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCGGGCGAAATATAGAAATACTGCAACAGACGGATGATTGCACCAATGCAGCGGTAAAGGGCGCTGGAGGCGGCAAAgg > 6:155928/1‑149 (MQ=255) ttGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACgta > 4:22109/1‑149 (MQ=255) gCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACgtag < 3:167812/148‑1 (MQ=255) gCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGtagtag < 7:279485/144‑1 (MQ=255) gCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGtagtag > 8:279485/1‑144 (MQ=255) gCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCaaa > 6:228168/1‑149 (MQ=255) tGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACgtagt > 4:40119/1‑140 (MQ=255) tGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACgtagt < 3:40119/140‑1 (MQ=255) aCATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGccc < 3:119019/149‑1 (MQ=255) acctgattcattTCGGC‑GCCGAGCAGCAGCACGTCAGCCGAAATATAGAAATAGAGCAAGAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTAGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCATGGAGGCCACGa < 4:38003/143‑1 (MQ=255) aCCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGa < 3:22109/148‑1 (MQ=255) ccGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATc > 5:178990/1‑149 (MQ=255) ttcattTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATccacac > 8:53715/1‑149 (MQ=255) attTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCt > 2:190646/1‑106 (MQ=255) attTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCt < 1:190646/106‑1 (MQ=255) attTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATccacacca > 2:291722/1‑148 (MQ=255) tCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACCGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGGCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACTATCCACACCACaa < 5:155928/148‑1 (MQ=255) cGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGATAAGCCCAGGGAGGCCACGATCCACACCACAAc < 8:71195/148‑1 (MQ=255) gctgcCGTGCTGCTGCTCGGC‑GCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGc > 8:170349/4‑149 (MQ=255) gc‑gcCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGc > 6:274472/1‑149 (MQ=255) gc‑gcCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGc > 8:127895/1‑149 (MQ=255) gcCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGc > 3:159930/1‑147 (MQ=255) gcCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGCCa < 5:228168/149‑1 (MQ=255) gcagcagcaCGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGCCAGCAcc < 6:178990/149‑1 (MQ=255) agcaCGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACGACCGCCAGCACCGAGcc > 6:120178/1‑149 (MQ=255) | TATGAGGCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGCCAGCACCGAGCC > NC_002947/5181006‑5181300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |