Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,182,066 C→A 13.1% intergenic (‑124/+30) PP_4560 ← / ← csbD ribonuclease BN/stress response protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,182,0660CA13.1% 125.0 / 10.2 52intergenic (‑124/+30)PP_4560/csbDribonuclease BN/stress response protein
Reads supporting (aligned to +/- strand):  ref base C (25/20);  new base A (5/2);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

CCGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACACC  >  NC_002947/5181920‑5182212
                                                                                                                                                  |                                                                                                                                                   
ccGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCAc                                                                                                                                                   >  5:206288/1‑149 (MQ=255)
     cAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCgag                                                                                                                                              <  3:252080/149‑1 (MQ=255)
      aGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCgag                                                                                                                                              >  8:213595/1‑148 (MQ=255)
       ggTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCgcg                                                                                                                                              >  5:257882/1‑145 (MQ=255)
       ggTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGcgaa                                                                                                                                            <  6:257882/149‑1 (MQ=255)
        gTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCgag                                                                                                                                              >  1:80402/1‑146 (MQ=255)
        gTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGagaag                                                                                                                                           <  4:226082/149‑1 (MQ=255)
            gggAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGc                                                                                                                                       >  1:283619/1‑149 (MQ=255)
            gggAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGc                                                                                                                                       <  6:206288/149‑1 (MQ=255)
                        gCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCt                                                                                                                            >  2:49747/1‑148 (MQ=255)
                           tctcCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCt                                                                                                                            >  8:87759/1‑145 (MQ=255)
                           tctcCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCt                                                                                                                            <  7:87759/145‑1 (MQ=255)
                                tttCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCtcaagtcaag                                                                                                                   >  8:170040/1‑149 (MQ=255)
                                     ccgcTTGATCGCTACACCGGCAAGTCTAATACACCGTCCCCTAGCTGGGCTGCTCCCGGCCGTGGTTGAAGAATTTCCTTACTAATAAGTCCCGGCCTCTCCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGcttc                                                                                                              <  6:281503/149‑1 (MQ=255)
                                                   cacaGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCACGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCAAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCt                                                                                                 >  5:248838/1‑148 (MQ=255)
                                                      aGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGTTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTca                                                                                              >  2:13134/1‑148 (MQ=255)
                                                        gCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCGGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACAt                                                                                           >  7:245556/1‑149 (MQ=255)
                                                        gCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCACGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTATTCGCGGGTTAACCCGAGAAGAGGCCGGAACCGCATCAAGTCAAGGCTTATTCACCGAATCCTTCACAt                                                                                           >  7:194036/1‑149 (MQ=255)
                                                             tCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑c                                                                                       >  6:295787/1‑147 (MQ=255)
                                                             tCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑c                                                                                       <  5:295787/147‑1 (MQ=255)
                                                                   aGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAgg                                                                                                                  >  5:245060/1‑115 (MQ=255)
                                                                         tGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACGGCATCCTTCACATTT‑CCCTTGACCtgctgc                                                                         >  4:67745/1‑149 (MQ=255)
                                                                             cGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGcgcct                                                                     <  2:80402/149‑1 (MQ=255)
                                                                               aGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGcgcctcg                                                                   >  7:1922/1‑149 (MQ=255)
                                                                                     gctgctCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCttt                                                              >  4:291465/1‑148 (MQ=255)
                                                                                       tgctCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGAGCCTCGCCttt                                                              >  7:52746/1‑146 (MQ=255)
                                                                                            ccGGCCGTGTTTTATTAATTTGCTTACTATAAAGTGCCGGCCTCTTCGCGGGTTCACCTGAGAAGCGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCt                                                      <  8:245556/149‑1 (MQ=255)
                                                                                            ccGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGAGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTc                                                        >  5:36962/1‑147 (MQ=255)
                                                                                            ccGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGAGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTc                                                        <  6:36962/147‑1 (MQ=255)
                                                                                             cGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCATCAAGTCAAGGCTTCTTCACCGCCTGCTTCACATTT‑GCCGTGGCGTGCTGGGCCTAGCCTGGGAGCTCCTg                                                     >  8:231129/1‑149 (MQ=255)
                                                                                                ccGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTgcgc                                                  >  4:131735/1‑149 (MQ=255)
                                                                                                 cGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCgcc                                                 <  3:131735/149‑1 (MQ=255)
                                                                                                  gTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCgcc                                                 <  1:49747/149‑1 (MQ=255)
                                                                                                       tGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCgccttgcct                                           >  4:122938/1‑149 (MQ=255)
                                                                                                         aagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCAAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCgccttgcctt                                          <  6:248838/148‑1 (MQ=255)
                                                                                                         aagaaTTTGCTTACGAATAAGTGCCGGCCGCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTc                                         <  7:213595/149‑1 (MQ=255)
                                                                                                                    tACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCt                              >  7:39143/1‑149 (MQ=255)
                                                                                                                       gAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCtttt                           >  6:5825/1‑149 (MQ=255)
                                                                                                                         aTAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCg                         <  7:170040/149‑1 (MQ=255)
                                                                                                                                ccGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCg                   <  3:122938/148‑1 (MQ=255)
                                                                                                                                ccGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCg                   <  2:87140/148‑1 (MQ=255)
                                                                                                                                  ggCCTCTTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGt                 >  8:221862/1‑148 (MQ=255)
                                                                                                                                    ccTCTTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGGTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCAc              >  5:44457/1‑149 (MQ=39)
                                                                                                                                     ctctTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGcgcctcgc                                                                  <  7:19910/96‑1 (MQ=38)
                                                                                                                                     ctctTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGcgcctcgc                                                                  >  8:19910/1‑96 (MQ=38)
                                                                                                                                       ctTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCtt           >  2:110847/1‑149 (MQ=255)
                                                                                                                                       ctTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCtt           >  1:300876/1‑149 (MQ=255)
                                                                                                                                          cgcgGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGc         <  5:5825/148‑1 (MQ=255)
                                                                                                                                          cgcgGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGc         <  1:13134/148‑1 (MQ=255)
                                                                                                                                              ggTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAaca    <  3:291465/149‑1 (MQ=255)
                                                                                                                                              ggTCCACCCCACAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAaca    <  2:224985/149‑1 (MQ=255)
                                                                                                                                                ttCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACAcc  >  7:73274/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTT‑CCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACACC  >  NC_002947/5181920‑5182212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑