Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,184,117 C→T 7.9% A85V (GCC→GTC)  PP_4564 → lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,184,1170CT 7.9% 142.8 / 2.3 51A85V (GCC→GTC) PP_4564lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (22/24);  new base T (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

GTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCC  >  NC_002947/5183977‑5184265
                                                                                                                                             |                                                                                                                                                    
gTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCgacgac                                                                                                                                               <  2:25268/148‑1 (MQ=255)
 tCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCgacgac                                                                                                                                               >  5:289307/1‑147 (MQ=255)
       aGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGT‑CGTCGGCGACCATGTc                                                                                                                                         <  4:255960/147‑1 (MQ=255)
        gCGACTTCCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCAcc                                                                                                                                      <  4:1187/149‑1 (MQ=255)
        gCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGT‑CGTCGGCGACCATGTCAcg                                                                                                                                      <  7:28204/149‑2 (MQ=255)
           aCTACCTGGTCATGTACCGCGAGCAAAGGGCGCTGCTGTATGTTCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCGGGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGTCGACCAGGTCACCGAc                                                                                                                                   <  6:290472/149‑1 (MQ=255)
            cTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGAc                                                                                                                                   >  2:183863/1‑148 (MQ=255)
             tACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGAc                                                                                                                                   >  8:153748/1‑147 (MQ=255)
               ccTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAgg                                                                                                                               >  7:54307/1‑149 (MQ=255)
                cTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAgga                                                                                                                              >  8:220145/1‑148 (MQ=255)
                    aCATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTg                                                                                                                          >  5:288046/1‑149 (MQ=255)
                     cATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCCGCCGACGACCAGGTCACCGACCAGGCCCTg                                                                                                                          <  1:183863/149‑1 (MQ=255)
                     cATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTgc                                                                                                                         <  7:220145/149‑1 (MQ=255)
                           ccGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa                                                                                                                   <  4:203860/149‑1 (MQ=255)
                           ccGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa                                                                                                                   <  6:289307/149‑1 (MQ=255)
                           ccGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa                                                                                                                   <  7:292070/149‑1 (MQ=255)
                              cgAGCAAAGGGCGCTGCTGGATGATCGGCTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCa                                                                                                                >  8:24169/1‑149 (MQ=255)
                                     aGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGTTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGa                                                                                                         <  6:136016/149‑1 (MQ=255)
                                        gcgcTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGt                                                                                                      <  8:54307/149‑1 (MQ=255)
                                          gctgctGGATGATCGGCTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTcc                                                                                                    <  7:24169/149‑1 (MQ=255)
                                                    gatCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAAt                                                                                          >  5:52017/1‑149 (MQ=255)
                                                         gTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTc                                                                                     <  7:37503/149‑1 (MQ=255)
                                                              gcgAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTg                                                                                <  7:153748/149‑1 (MQ=255)
                                                                gAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTc                                                                                     >  1:97047/1‑142 (MQ=255)
                                                                gAGCACCTGGACTTGCATTTCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTTAGGCAATTGGTc                                                                                     <  2:97047/142‑1 (MQ=255)
                                                                   cACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGa                                                                           <  6:288046/149‑1 (MQ=255)
                                                                      ctggcctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGc                                                                              >  7:210084/1‑143 (MQ=255)
                                                                      ctggcctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGc                                                                              <  8:210084/143‑1 (MQ=255)
                                                                         gcctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAAt                                                                     >  6:21995/1‑149 (MQ=255)
                                                                                aTTGCAAGACTCAACTGCCCGCCTACCTCGACTGGCTCGACCCGCTACGTGACATGGT‑CGCCGCCGCCCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCt                                                              >  1:77083/1‑149 (MQ=255)
                                                                                        aCTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCAc                                                      >  7:54178/1‑149 (MQ=255)
                                                                                               tGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGGCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAActg                                               >  1:216501/1‑149 (MQ=255)
                                                                                                         ccTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGc                                      <  5:21995/148‑1 (MQ=255)
                                                                                                         ccTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCa                                     >  7:63019/1‑149 (MQ=255)
                                                                                                           tCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCAt                                    >  8:249625/1‑148 (MQ=255)
                                                                                                             gACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGa                                  <  6:52017/148‑1 (MQ=255)
                                                                                                              aCTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGc                                <  2:216501/149‑1 (MQ=255)
                                                                                                              aCTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGc                                <  8:54178/149‑1 (MQ=255)
                                                                                                               cTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGATctgct                                             <  8:283065/135‑1 (MQ=255)
                                                                                                               cTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGATctgct                                             >  7:283065/1‑135 (MQ=255)
                                                                                                                tGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGa                              >  6:238674/1‑149 (MQ=255)
                                                                                                                             tACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAgcg                 >  7:45415/1‑149 (MQ=255)
                                                                                                                               ggTGACCTGGT‑CGTCGGCGACCATGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAgcg                 >  2:289097/2‑147 (MQ=255)
                                                                                                                                 tGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCg                                                                            <  4:120421/86‑1 (MQ=255)
                                                                                                                                 tGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCg                                                                            >  3:120421/1‑86 (MQ=255)
                                                                                                                                 tGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAgcg                 >  1:266007/1‑145 (MQ=255)
                                                                                                                                     ctGGT‑CGTCGGCGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCTACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCg         >  6:32964/2‑149 (MQ=255)
                                                                                                                                     aTGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCg         >  3:276292/1‑149 (MQ=255)
                                                                                                                                     aTGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCg         >  2:166363/1‑149 (MQ=255)
                                                                                                                                      tGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGt        <  5:238674/149‑1 (MQ=255)
                                                                                                                                        gt‑cgtcGGCGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCTACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGa      <  5:32964/149‑1 (MQ=255)
                                                                                                                                         t‑cgtcgGCGACCATGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAg     <  1:289097/149‑1 (MQ=255)
                                                                                                                                         t‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAg     <  8:63019/149‑1 (MQ=255)
                                                                                                                                             ccGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGcc  >  4:297723/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGT‑CGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCC  >  NC_002947/5183977‑5184265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑