Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 388,722 T→A 8.2% L198Q (CTG→CAG)  soxB → sarcosine oxidase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947388,7220TA 8.2% 160.1 / 3.2 57L198Q (CTG→CAG) soxBsarcosine oxidase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (25/27);  new base A (2/3);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

CTGCCCAGGTTGCGGCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCGGTAACTCCGGGCACATGGCCAAGC  >  NC_002947/388578‑388868
                                                                                                                                                |                                                                                                                                                  
cTGCCCAGGTTGCGGCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGc                                                                                                                                                <  7:257812/149‑1 (MQ=255)
   cccAGGTTGCGGCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTc                                                                                                                                             <  8:198405/149‑1 (MQ=255)
    ccAGGTTGCGGCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGc                                                                                                                                                <  4:22736/145‑1 (MQ=255)
    ccAGGTTGCGGCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGc                                                                                                                                                >  3:22736/1‑145 (MQ=255)
    ccAGGTTGCGGCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTc                                                                                                                                             >  7:67871/1‑148 (MQ=255)
     cAGGTTGCGGCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCAGGGCGTCgg                                                                                                                                           <  8:246330/149‑2 (MQ=255)
      aGGTTGCGGCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCAGGGCGTCgg                                                                                                                                           >  3:118510/1‑147 (MQ=255)
       ggTTGCGGCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCAGGGCGTCggca                                                                                                                                         <  4:118510/149‑4 (MQ=255)
        gTTGCGGCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACGGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCt                                                                                                                                        <  7:199954/149‑1 (MQ=255)
             ggCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGa                                                                                                                                      <  6:264708/146‑1 (MQ=255)
             ggCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGa                                                                                                                                      >  5:264708/1‑146 (MQ=255)
             ggCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATc                                                                                                                                    >  4:181571/1‑148 (MQ=255)
               ccGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAg                                                                                                                                 >  4:117797/1‑149 (MQ=255)
                    aTCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCAGGGCGTCggca                                                                                                                                         <  1:119122/136‑4 (MQ=255)
                    aTCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCCCCGCCCAGGgcggcggca                                                                                                                                         >  2:119122/1‑133 (MQ=255)
                     tCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAAcc                                                                                                                           >  1:29611/1‑149 (MQ=255)
                        cGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGaa                                                                                                                        <  2:294924/149‑1 (MQ=255)
                         ctACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTTGTGGCGTTGCCCGTCACGATGCCGTGGCCTGTGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGaa                                                                                                                        <  7:5866/147‑1 (MQ=255)
                             cTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGa                                                                                                                                      <  2:228801/130‑1 (MQ=255)
                             cTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGa                                                                                                                                      >  1:228801/1‑130 (MQ=255)
                             cTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGAt                                                                                                                   >  6:50665/1‑149 (MQ=255)
                               ggACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCg                                                                                                                 >  3:27920/1‑149 (MQ=255)
                                   tGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCtt                                                                                                             >  5:298079/1‑149 (MQ=255)
                                      tCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCg                                                                                                          >  2:198868/1‑149 (MQ=255)
                                        gaagaaCACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGc                                                                                                         >  6:124430/1‑148 (MQ=255)
                                        gaagaaCACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGc                                                                                                         >  5:66146/1‑148 (MQ=255)
                                        gaagaaCACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGc                                                                                                         <  5:50665/148‑1 (MQ=255)
                                        gaagaaCACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGc                                                                                                         >  5:229208/1‑148 (MQ=255)
                                        gaagaaCACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCAGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGc                                                                                                         >  8:124568/1‑148 (MQ=255)
                                         aagaaCACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCaa                                                                                                       <  5:297790/149‑1 (MQ=255)
                                               acCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGaaaa                                                                                                 <  4:27920/149‑1 (MQ=255)
                                                   gCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAAc                                                                                                                            <  4:230127/118‑1 (MQ=255)
                                                   gCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAAc                                                                                                                            >  3:230127/1‑118 (MQ=255)
                                                   gCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGgc                                                                                             >  5:67151/1‑149 (MQ=255)
                                                           aTCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCAt                                                                                     <  3:181571/149‑1 (MQ=255)
                                                            tCCTCGGCGCCACCGTACAGCGCCGTGGTTGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCTGTCATc                                                                                    <  6:229208/149‑1 (MQ=255)
                                                              cTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCg                                                                                   <  8:67871/148‑1 (MQ=255)
                                                                 ggCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGgt                                                                                 <  5:124430/147‑1 (MQ=255)
                                                                           tACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACcaac                                                                     <  6:67151/149‑1 (MQ=255)
                                                                               gcgcCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAg                                                                 <  6:294057/149‑1 (MQ=255)
                                                                                cgcCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAgg                                                                <  6:298079/149‑1 (MQ=255)
                                                                                 gcCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAGGc                                                               <  7:124568/149‑1 (MQ=255)
                                                                                            gTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTgg                                                                                                                                                  <  7:196483/55‑1 (MQ=255)
                                                                                            gTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTgg                                                                                                                                                  >  8:196483/1‑55 (MQ=255)
                                                                                               gCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAGGCTTCATCGGCGcc                                                   >  6:128286/1‑147 (MQ=255)
                                                                                                 ccGTCACGATGCCGTGGCCTGGGACTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAGGCTTCATCGGCGCCAAgc                                               >  7:193574/1‑149 (MQ=255)
                                                                                                  cGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAGGCTTCATCGGCGCCAAgcg                                              >  3:251292/1‑149 (MQ=255)
                                                                                                            ccGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAGTCTTCATCGGCGCCAAGCGCGTCGGCGTg                                    <  1:198868/149‑1 (MQ=255)
                                                                                                            ccGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTg                                    <  2:29611/149‑1 (MQ=255)
                                                                                                             cGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGaaa                                                                          >  7:211106/1‑110 (MQ=255)
                                                                                                             cGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGaaa                                                                          <  8:211106/110‑1 (MQ=255)
                                                                                                                  ccTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCAcc                              >  5:212358/1‑149 (MQ=255)
                                                                                                                    tGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCAccgc                            >  1:192039/1‑149 (MQ=255)
                                                                                                                       ggCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCgg                         <  6:66146/149‑1 (MQ=255)
                                                                                                                        gCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCGGt                        <  5:128286/149‑1 (MQ=255)
                                                                                                                                        cGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCGGTAACTCCGGGCACATgg        <  2:192039/149‑1 (MQ=255)
                                                                                                                                              ccTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCGGTAACTCCGGGCACATGGCCAAGc  >  5:55995/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CTGCCCAGGTTGCGGCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACCTGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGTGGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCGGTAACTCCGGGCACATGGCCAAGC  >  NC_002947/388578‑388868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑