Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,187,972	A→T	7.8%	E159D (GAA→GAT)	PP_4567 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,187,972	0	A	T	7.8%	189.5 / 4.8	64	E159D (GAA→GAT)	PP_4567	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/33);  new base T (3/2);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CAGCATTGCTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCG  >  NC_002947/5187827‑5188117                                                                                                                                                    |                                                                                                                                                  cagcaTTGCTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGc                                                                                                                                                <  3:39945/149‑1 (MQ=255)   gcaTTGCTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCgt                                                                                                                                              <  8:225502/149‑1 (MQ=255)   gcaTTGCTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCgt                                                                                                                                              <  7:90119/149‑1 (MQ=255)            gATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTgcgc                                                                                                                                       <  1:178483/147‑1 (MQ=255)            gATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGcgcca                                                                                                                                     <  5:179779/149‑1 (MQ=255)                    cTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATcc                                                                                                                             <  2:58879/149‑1 (MQ=255)                      gcgcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGATGGCGTGGCGCAACACGCCTTcctc                                                                                                                           >  4:12075/1‑147 (MQ=255)                       cgcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGATGGCGTGGCGCAACACGCCTTCCTCg                                                                                                                          <  3:12075/149‑1 (MQ=255)                       cgcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATcccc                                                                                                                           <  8:19708/148‑1 (MQ=255)                        gcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGGGGATGGCGTGGCGCAACACGCCTTCCTCg                                                                                                                          >  8:180560/1‑148 (MQ=38)                         ccTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGaa                                                                                                                        <  8:159194/149‑1 (MQ=255)                             tgAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGGCTATTCTTACATG‑TAACCCACCCCCAAACCCGCCTGGTGGACTTACAGGAAGGCGGTTTGCCCCCCGCCAACCCCGAagag                                                                                                                     >  1:246069/1‑149 (MQ=255)                             tgAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAagt                                                                                                                      <  7:180560/147‑2 (MQ=38)                                aaCCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTcc                                                                                                                 <  8:121225/149‑1 (MQ=255)                                   cGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAag                                                                                                                       <  2:245627/140‑1 (MQ=255)                                   cGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAag                                                                                                                       >  1:245627/1‑140 (MQ=255)                                            aGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTAc                                                                                                     >  7:38546/1‑149 (MQ=255)                                               gAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCt                                                                                                  >  5:5595/1‑149 (MQ=255)                                                 aGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTc                                                                                                >  5:231083/1‑149 (MQ=255)                                                 aGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTc                                                                                                >  2:60008/1‑149 (MQ=255)                                                  gATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCg                                                                                               <  5:143060/149‑1 (MQ=255)                                                  gATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCg                                                                                               <  6:5595/149‑1 (MQ=255)                                                   aTGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCgg                                                                                              <  6:151379/149‑1 (MQ=255)                                                     gTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGc                                                                                             >  6:298909/1‑148 (MQ=255)                                                       tGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGc                                                                                                   <  2:65975/140‑1 (MQ=255)                                                       tGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGc                                                                                                   >  1:65975/1‑140 (MQ=255)                                                                aGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCgcggc                                                                                 <  2:246069/149‑1 (MQ=255)                                                                       gccgcAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTg                                                                          >  2:142914/1‑149 (MQ=255)                                                                       gccgcAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTg                                                                          <  1:21281/149‑1 (MQ=255)                                                                          gcAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCg                                                                        >  7:132000/1‑148 (MQ=255)                                                                                     tcgATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAaca                                                                   >  2:7145/1‑142 (MQ=255)                                                                                     tcgATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAaca                                                                   <  1:7145/142‑1 (MQ=255)                                                                                        aTACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcagcag                                                         <  5:133959/149‑1 (MQ=255)                                                                                         tACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACagcagc                                                        <  1:142914/149‑1 (MQ=255)                                                                                            ccT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGc                                                                                                               >  3:70652/1‑91 (MQ=255)                                                                                            ccT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGc                                                                                                               <  4:70652/91‑1 (MQ=255)                                                                                            ccT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGccc                                                      >  4:74558/1‑148 (MQ=255)                                                                                                gCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTg                                                                          <  1:254913/125‑1 (MQ=255)                                                                                                gCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTg                                                                          >  2:254913/1‑125 (MQ=255)                                                                                                    tac‑tacCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCAc                                                <  3:74558/147‑1 (MQ=255)                                                                                                           cTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGcc                                        >  1:208532/1‑149 (MQ=255)                                                                                                             gCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGccc                                       >  1:187062/1‑148 (MQ=255)                                                                                                              cTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGc                                         <  8:136924/145‑1 (MQ=255)                                                                                                              cTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGc                                         >  7:136924/1‑145 (MQ=255)                                                                                                                  cccAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGcc                                 <  5:298909/149‑1 (MQ=255)                                                                                                                    cAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCca                               <  2:187062/149‑1 (MQ=255)                                                                                                                    cAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCca                               <  1:60008/149‑1 (MQ=255)                                                                                                                     aGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACagcagc                                                        >  1:133398/1‑123 (MQ=255)                                                                                                                     aGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACagcagc                                                        <  2:133398/123‑1 (MQ=255)                                                                                                                          gAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGc                         <  2:103192/149‑1 (MQ=255)                                                                                                                           aaGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCt                        >  6:76103/1‑149 (MQ=255)                                                                                                                             gCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCt                        >  2:269886/1‑147 (MQ=255)                                                                                                                             gCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGa                      <  6:177602/149‑1 (MQ=255)                                                                                                                                ggCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCg                   <  8:38546/149‑1 (MQ=255)                                                                                                                                          tcTTCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCg         >  5:220202/2‑149 (MQ=38)                                                                                                                                            ttCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGt       >  5:178449/1‑149 (MQ=255)                                                                                                                                            ttCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGACCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGAGCCAGGCGGt       >  6:143737/1‑149 (MQ=255)                                                                                                                                              cGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAgg                          <  7:91711/128‑1 (MQ=255)                                                                                                                                              cGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAgg                          >  8:91711/1‑128 (MQ=255)                                                                                                                                                aGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTAcc   <  6:231083/149‑1 (MQ=255)                                                                                                                                                aGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTAcc   >  5:86265/1‑149 (MQ=255)                                                                                                                                                aGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAAAACACCCAAACCACAAACAGCCCCAAGCCCACCAGCCAGAACGCTGCACGGCGGttcac   >  8:192172/1‑146 (MQ=255)                                                                                                                                                  gAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGACCGGTGCCAGGCGGTTACCg  >  3:161406/1‑148 (MQ=255)                                                                                                                                                  gAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGACCGGTGCCAGGCGGTTACCg  <  4:161406/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CAGCATTGCTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCT‑GCTCTAC‑TACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCG  >  NC_002947/5187827‑5188117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑