Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,257,704 G→T 10.0% P33H (CCC→CAC)  PP_4634 ← alpha/beta family hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,257,7040GT10.0% 204.6 / 11.3 70P33H (CCC→CAC) PP_4634alpha/beta family hydrolase
Reads supporting (aligned to +/- strand):  ref base G (32/31);  new base T (4/3);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

ACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAG  >  NC_002947/5257556‑5257848
                                                                                                                                                    |                                                                                                                                                
aCGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTggg                                                                                                                                                  <  5:151322/149‑1 (MQ=255)
aCGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTggg                                                                                                                                                  >  4:266137/1‑149 (MQ=255)
 cGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTgggg                                                                                                                                                 <  1:286754/149‑1 (MQ=255)
     gCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCa                                                                                                                                             <  8:119051/149‑1 (MQ=255)
         aGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCCCCAGCGCCCGCTGGTGATCatcaa                                                                                                                                         <  6:114111/149‑5 (MQ=255)
                  ggATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCCCCAGCGCCCGCTGGTGa                                                                                                                                                <  5:128847/133‑1 (MQ=255)
                  ggATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCCCCAGCGCCCGCTGGTGa                                                                                                                                                >  6:128847/1‑133 (MQ=255)
                    aTTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGgcgc                                                                                                                              >  3:129928/1‑149 (MQ=255)
                      tCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCCCCAGCGCCCGCTGGTGATCat                                                                                                                                            >  7:32336/1‑132 (MQ=255)
                      ncGCCNANTCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTNATAGNGGCAGCGTACCGAAGTGGCAGCGTTGATGATCCCCAGCGCCCGCTGGTGATCat                                                                                                                                            <  8:32336/132‑2 (MQ=255)
                        gCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCcag                                                                                                                           <  7:145783/148‑1 (MQ=255)
                        gCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCcag                                                                                                                           >  4:26672/1‑148 (MQ=255)
                                       gTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGGGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAgg                                                                                                            <  3:70519/148‑1 (MQ=255)
                                       gTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAgg                                                                                                            <  5:162576/148‑1 (MQ=255)
                                       gTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGc                                                                                                           >  3:295535/1‑149 (MQ=255)
                                       gTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGc                                                                                                           >  5:263489/1‑149 (MQ=255)
                                          gTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGt                                                                                                        <  4:225604/149‑1 (MQ=255)
                                             ggTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTAt                                                                                                      <  4:295535/148‑1 (MQ=255)
                                                 aGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTc                                                                                                 >  3:222982/1‑149 (MQ=255)
                                                 aGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTc                                                                                                 <  2:283995/149‑1 (MQ=255)
                                                      aTCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCcg                                                                                            >  6:159830/1‑149 (MQ=255)
                                                       tCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAgg                                                                                                            >  2:284597/1‑132 (MQ=255)
                                                       tCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAgg                                                                                                            <  1:284597/132‑1 (MQ=255)
                                                          cAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCggg                                                                                        <  1:4688/149‑1 (MQ=255)
                                                            gCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGt                                                                                      <  3:26672/149‑1 (MQ=255)
                                                             ccGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTg                                                                                     >  4:138388/1‑149 (MQ=255)
                                                               gTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGc                                                                                   >  3:82184/1‑149 (MQ=255)
                                                                tGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGCAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGc                                                                                   >  8:95671/1‑148 (MQ=255)
                                                                    cGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCgg                                                                                               <  4:231244/132‑1 (MQ=255)
                                                                    cGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCgg                                                                                               >  3:231244/1‑132 (MQ=255)
                                                                    cGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAAt                                                                              >  4:256771/1‑149 (MQ=255)
                                                                       aCAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATggg                                                                           <  5:222559/149‑1 (MQ=255)
                                                                       aCAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATggg                                                                           <  4:82184/149‑1 (MQ=255)
                                                                          ggtcggcGGCACAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGCTCCCCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTa                                                                        <  4:222982/143‑1 (MQ=255)
                                                                           gTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTgg                                                                                    <  3:292940/136‑1 (MQ=255)
                                                                           gTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTgg                                                                                    >  4:292940/1‑136 (MQ=255)
                                                                           gTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAg                                                                       >  2:53371/1‑149 (MQ=255)
                                                                             aGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGaa                                                                                                                      >  2:295306/1‑100 (MQ=255)
                                                                             aGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGaa                                                                                                                      <  1:295306/100‑1 (MQ=255)
                                                                             aGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCg                                                                     >  1:150990/1‑149 (MQ=255)
                                                                                cGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGt                                                                  >  7:97758/1‑149 (MQ=255)
                                                                                        cGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAg                                                          <  6:263489/149‑1 (MQ=255)
                                                                                              tAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACt                                                    >  6:93800/1‑149 (MQ=255)
                                                                                               aaTAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACt                                                    >  4:33133/1‑148 (MQ=255)
                                                                                                  aGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATg                                                >  5:297238/1‑149 (MQ=255)
                                                                                                   gCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATg                                                <  4:129928/148‑1 (MQ=255)
                                                                                                      gCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAgccgcc                                                                                                                >  1:10251/1‑81 (MQ=255)
                                                                                                      gCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAgccgcc                                                                                                                <  2:10251/81‑1 (MQ=255)
                                                                                                      gCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGgcg                                             >  4:21934/1‑148 (MQ=255)
                                                                                                          cGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGt                                        >  1:97438/1‑149 (MQ=255)
                                                                                                               cGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgt                                   >  4:141708/1‑149 (MQ=255)
                                                                                                                 aaGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGTCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttg                                 >  5:246127/1‑149 (MQ=255)
                                                                                                                   gTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttgtt                               <  4:72145/149‑1 (MQ=255)
                                                                                                                     ggCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttgttgt                             <  8:97758/149‑1 (MQ=255)
                                                                                                                      gCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTtgtg                            <  6:297238/149‑1 (MQ=255)
                                                                                                                      gCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTtgtg                            >  2:153687/1‑149 (MQ=255)
                                                                                                                       cAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGGTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTgc                           <  5:159830/149‑1 (MQ=255)
                                                                                                                           caacctgatCCCCAGCGCCCGCTGGTGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATg                                         >  6:236232/6‑131 (MQ=255)
                                                                                                                            ttGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAg                                                          <  6:41528/113‑1 (MQ=255)
                                                                                                                            ttGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAg                                                          >  5:41528/1‑113 (MQ=255)
                                                                                                                            aacctgatCCCCAGCGCCCGCTGGTGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCa                       >  8:141202/5‑148 (MQ=255)
                                                                                                                              gatgatCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCaaaa                    >  5:113825/1‑149 (MQ=255)
                                                                                                                                 gatCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTCTTGTTGTGAGCCAAAAc                   >  6:270553/1‑147 (MQ=255)
                                                                                                                                  atCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTGGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTa                 <  7:95671/148‑1 (MQ=255)
                                                                                                                                   tCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAg                <  3:266137/148‑1 (MQ=255)
                                                                                                                                    cACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGc               <  2:97438/148‑1 (MQ=255)
                                                                                                                                     aCCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCaa             <  2:150990/149‑1 (MQ=255)
                                                                                                                                     aCCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCa              >  6:123628/1‑148 (MQ=255)
                                                                                                                                         gCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGAt         <  3:256771/149‑1 (MQ=255)
                                                                                                                                           gggCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGgcgc                                            >  7:77636/1‑112 (MQ=255)
                                                                                                                                           gggCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGgcgc                                            <  8:77636/112‑1 (MQ=255)
                                                                                                                                               gcTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTg     >  8:251992/1‑147 (MQ=255)
                                                                                                                                                cTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAg  <  5:123628/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAG  >  NC_002947/5257556‑5257848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑