Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,272,185 G→A 6.1% A46T (GCT→ACT)  PP_4647 → transcriptional regulator, LuxR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,272,1850GA 6.1% 192.6 / 2.3 65A46T (GCT→ACT) PP_4647transcriptional regulator, LuxR family
Reads supporting (aligned to +/- strand):  ref base G (31/30);  new base A (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

TCAAATGCTTCACTGGAAACCCGAGTATCTTCGTGCGTTCGTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAGCTCGACGATGCCGGTGCTGTGGACCGAT  >  NC_002947/5272046‑5272325
                                                                                                                                           |                                                                                                                                            
tCAAATGCTTCACTGGAAACCCGAGTATCTTCGTGCGTTCGTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACaaa                                                                                                                                     >  7:276076/1‑149 (MQ=255)
               gAAACCCGAGTATCTTCGTGCGTTCGTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTa                                                                                                                        <  1:161025/147‑1 (MQ=255)
               gAAACCCGAGTATCTTCGTGCGTTCGTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTAc                                                                                                                       <  1:62038/148‑1 (MQ=255)
               gAAACCCGAGTATCTTCGTGCGTTCGTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTAc                                                                                                                       <  2:72582/148‑1 (MQ=255)
                    ccGAGTATCTTCGTGCGTTCGTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTa                                                                                                                  >  5:65699/1‑148 (MQ=255)
                    ccGAGTATCTTAGTGAGTTCGTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTa                                                                                                                  >  3:258765/1‑148 (MQ=255)
                     cGAGTATCTTCGTGCGTTCGTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACa                                                                                                                <  1:13270/149‑1 (MQ=255)
                           tCTTCGTGCGTTCGTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAAc                                                                                                           >  3:201896/1‑148 (MQ=255)
                               cGTGCGTTCGTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTAcc                                                                                                       <  6:65699/148‑1 (MQ=255)
                                        gTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTa                                                                                                                  <  4:280308/128‑1 (MQ=255)
                                        gTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTa                                                                                                                  >  3:280308/1‑128 (MQ=255)
                                        gTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAagtgagtg                                                                                             >  8:41610/1‑149 (MQ=255)
                                        gTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAagtgagt                                                                                              <  4:227825/148‑1 (MQ=255)
                                           agcgagcgCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAagt                                                                                                  <  1:214714/141‑1 (MQ=255)
                                           agcgagcgCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAagt                                                                                                  <  1:218931/141‑1 (MQ=255)
                                           agcgagcgCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAagt                                                                                                  >  2:214714/1‑141 (MQ=255)
                                           agcgagcgCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAagt                                                                                                  >  2:218931/1‑141 (MQ=255)
                                            gcgagcgCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGc                                                                                                              <  8:143252/128‑1 (MQ=255)
                                            gcgagcgCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGc                                                                                                              >  7:143252/1‑128 (MQ=255)
                                            gcgagcgCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTAc                                                                                                        >  8:88578/1‑134 (MQ=255)
                                            gcgagcgCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATc                                                                                         <  4:211661/149‑1 (MQ=255)
                                             cgagcgCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATc                                                                                         >  8:245772/1‑148 (MQ=255)
                                               agcgCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCGTTCAGATCGCTACACAAAc                                                                                                                                    >  3:21387/1‑103 (MQ=255)
                                               agcgCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCGTTCAGATCGCTACACAAAc                                                                                                                                    <  4:21387/103‑1 (MQ=255)
                                                  gcAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACAAGGATTACCTTGGGCTCAACATCCGCAATCAGATCGCTACACAAACCCCAAGCCTGTACCACAACAGCAACAACCCAAGAGAGAGCATCGAgcg                                                                                    >  7:105150/1‑148 (MQ=255)
                                                      cccGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTAc                                                                                >  6:28957/1‑148 (MQ=255)
                                                       ccGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCa                                                                              <  5:28957/149‑1 (MQ=255)
                                                       ccGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGAACTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCa                                                                              <  8:276076/149‑1 (MQ=255)
                                                        cGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAg                                                                             >  8:36198/1‑149 (MQ=255)
                                                            gAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAgcgc                                                                          >  7:32840/1‑148 (MQ=255)
                                                               gCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGcgac                                                                       >  2:102640/1‑148 (MQ=255)
                                                                cTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGcgacga                                                                     <  1:279866/149‑1 (MQ=255)
                                                                      gACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGCGCGCTGCCAGCGCGACGACTTCTa                                                               >  8:145757/1‑149 (MQ=255)
                                                                       aCATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTAc                                                              <  7:41610/149‑1 (MQ=255)
                                                                       aCATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTAc                                                              <  7:245772/149‑1 (MQ=255)
                                                                         aTCGCAGTCCATCTGGCTCAGGACCTGGACATGGAATACCTTGGGCTGAACATCAGCATTAAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGAGACGACTTCTAc                                                              >  5:285313/1‑147 (MQ=255)
                                                                          tCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAg                                                           >  3:71989/1‑149 (MQ=255)
                                                                                tCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGcga                                                                        >  8:13005/1‑130 (MQ=255)
                                                                                tCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGcga                                                                        <  7:13005/130‑1 (MQ=255)
                                                                                 ccATCTGGCTCAGGACCTGGACATGGATAACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGAcc                                                    >  6:128827/1‑149 (MQ=255)
                                                                                  cATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGAccc                                                   >  2:231861/1‑149 (MQ=255)
                                                                                       ggCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGAt                                                                                          <  1:3385/105‑1 (MQ=255)
                                                                                       ggCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGAt                                                                                          >  2:3385/1‑105 (MQ=255)
                                                                                        gCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCTGATCGCTACAGATACGCCAAGGCTGTTCCTCTACAGCTACTACCCAAGTGAGTGGATCGAGCGGTACCAGCGCTACGACTTCTACAAGCAGGACCcggcgg                                              <  6:285313/148‑1 (MQ=255)
                                                                                        gCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCcggc                                                <  1:231861/146‑1 (MQ=255)
                                                                                          tCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGgcggcg                                            >  2:28657/1‑148 (MQ=255)
                                                                                            aGGACCTGGACATGCATTACCTTGGGCTGAACATCCGCAATCAGAGCGCTACCCAAACGCCTAGGCGGTAGCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTTCCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAAc                                         <  8:248825/149‑1 (MQ=255)
                                                                                                ccTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGa                                     <  5:128827/149‑1 (MQ=255)
                                                                                                ccTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGa                                     <  1:28657/149‑1 (MQ=255)
                                                                                                   ggACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGa                                     >  4:138614/1‑146 (MQ=255)
                                                                                                   ggACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGa                                     <  3:138614/146‑1 (MQ=255)
                                                                                                        tGGATTACCTTGGTCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAGc                             <  7:230057/149‑1 (MQ=255)
                                                                                                         ggATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAGc                             >  4:271455/1‑148 (MQ=255)
                                                                                                           aTTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCCAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAGCTc                           >  5:226929/1‑148 (MQ=255)
                                                                                                               ccTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAGCTcgacga                      >  7:180273/1‑149 (MQ=255)
                                                                                                                          aCATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAGCTCGACGATGCCGGTGCtg           <  8:180273/149‑1 (MQ=255)
                                                                                                                           cATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAGCTCGACGATGCCGGTGCtgt          <  4:201896/149‑1 (MQ=255)
                                                                                                                              ccGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAGCTCGACGATGCCGGTGCTGTGGa       >  2:88119/1‑149 (MQ=255)
                                                                                                                                gCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAg                              <  4:28047/124‑1 (MQ=255)
                                                                                                                                gCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAg                              >  3:28047/1‑124 (MQ=255)
                                                                                                                                   ttCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAGCTCGACGATGCCGGTGCTGTGGACCGAt  <  8:32840/149‑1 (MQ=255)
                                                                                                                                        aTCACTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAGc                             <  1:291670/117‑1 (MQ=255)
                                                                                                                                        aTCACTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAGc                             >  2:296238/1‑117 (MQ=255)
                                                                                                                                        aTCACTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAGc                             <  1:296238/117‑1 (MQ=255)
                                                                                                                                        aTCACTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAGc                             >  2:291670/1‑117 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TCAAATGCTTCACTGGAAACCCGAGTATCTTCGTGCGTTCGTCAGCGAGCGCAGCCCGCAGAAGCTATTCGACATCGCAGTCCATCTGGCTCAGGACCTGGACATGGATTACCTTGGGCTGAACATCCGCATTCAGATCGCTACACAAACGCCAAGGCTGTACCTCTACAGCAACTACCCAAGTGAGTGGATCGAGCGCTACCAGCGCGACGACTTCTACAAGCAGGACCCGGCGGCGAACCTGAGCCACAGCTCGACGATGCCGGTGCTGTGGACCGAT  >  NC_002947/5272046‑5272325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑