Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,300,650 C→G 7.3% P1041P (CCG→CCC)  recB ← Chi activated ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,300,6500CG 7.3% 216.1 / 6.2 68P1041P (CCG→CCC) recBChi activated ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease
Reads supporting (aligned to +/- strand):  ref base C (31/32);  new base G (3/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

CGCAGCTGGGCGCGCCAGACCGGGGTGGGTGTGGCGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGCTCTATCAGTTGAGGGTTAGCGCTGACCTCGCTGAAGC  >  NC_002947/5300503‑5300789
                                                                                                                                                   |                                                                                                                                           
cgcAGCTGGGCGCGCCAGACCGGGGTGGGTGTGGCGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGTGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGccc                                                                                                                                             <  6:184726/148‑1 (MQ=255)
     cTGGGCGCGCCAGACCGGGGTGGGTGTGGCGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGc                                                                                                                                       >  2:130072/1‑149 (MQ=255)
         gcgcgcCAGACCGGGGTGGGTGTGGCGTGCCACCAGGCGATCGAGTTGCTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGc                                                                                                                                    >  7:232126/1‑148 (MQ=255)
           gcgcCAGACCGGGGTGGGTGTGGCGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAAc                                                                                                                                 >  5:143968/1‑149 (MQ=255)
           gcgcCAGACCGGGGTGGGTGTGGCGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAAc                                                                                                                                 >  6:253844/1‑149 (MQ=255)
             gcCAGACCGGGGTGGGTGTGGCGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTTAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGc                                                                                                                               >  6:52662/1‑149 (MQ=255)
             gcCAGACCGGGGTGGGTGTGGCGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCGGGCAGCgaccaga                                                                                                                                <  2:250702/148‑7 (MQ=255)
             gcCAGACCGGGGTGGGTGTGGCGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCGGGCAGCgacca                                                                                                                                  >  1:250702/1‑142 (MQ=255)
                aGACCGGGGTGGGTGTGGCGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTc                                                                                                                            >  7:211943/1‑149 (MQ=255)
                              gtgGCGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCGGGCAGCgac                                                                                                                                    <  8:67508/127‑3 (MQ=255)
                              gtgGCGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCGGGCAGCgac                                                                                                                                    >  7:67508/1‑125 (MQ=255)
                               tgGCGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCt                                                                                                              <  7:31889/148‑1 (MQ=255)
                               tgGCGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTg                                                                                                             >  2:199786/1‑149 (MQ=255)
                                  cGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCAt                                                                                                          >  6:179301/1‑149 (MQ=255)
                                      ccaccaGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAg                                                                                                      <  1:130072/149‑1 (MQ=255)
                                        accaGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTg                                                                                                    >  6:145238/1‑149 (MQ=255)
                                         ccaGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTgg                                                                                                   <  2:211228/149‑1 (MQ=255)
                                            ggCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAACCGCTGCATCCAGTGGCt                                                                                                 >  7:112125/1‑148 (MQ=255)
                                             gCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCa                                                                                               <  5:179301/149‑1 (MQ=255)
                                                 tCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCTATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTg                                                                                                             >  5:162968/1‑131 (MQ=255)
                                                 tCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTg                                                                                                             <  6:162968/131‑1 (MQ=255)
                                                   gAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTg                                                                                          <  1:199786/148‑1 (MQ=255)
                                                         ttCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGc                                                                                                               >  7:98873/1‑121 (MQ=255)
                                                         ttCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGc                                                                                                               <  8:98873/121‑1 (MQ=255)
                                                               gTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCgg                                                                             <  3:294024/149‑1 (MQ=255)
                                                               gTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCgg                                                                             >  5:105858/1‑149 (MQ=255)
                                                                 cGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGcc                                                                           <  5:185042/149‑1 (MQ=255)
                                                                  gACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGGTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGGGGGGATCCAGCCGGccc                                                                          <  8:211943/149‑1 (MQ=255)
                                                                     ccGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCt                                                                                                 <  1:131810/123‑1 (MQ=255)
                                                                     ccGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCt                                                                                                 >  2:131810/1‑123 (MQ=255)
                                                                           gctggcGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCgcgg                                                                  <  2:132505/148‑1 (MQ=255)
                                                                              ggcGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTAAGGGTGGGGATACTGCCGGCCCAGTCGCGGCGAt                                                              >  8:14043/1‑149 (MQ=255)
                                                                               gcGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGAtt                                                             <  6:143968/149‑1 (MQ=255)
                                                                                 gAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGc                                                           <  8:232126/149‑1 (MQ=255)
                                                                                  aaCCAGAACTCCATTTTAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCa                                                          <  5:52662/149‑1 (MQ=255)
                                                                                  aaCCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCa                                                          <  2:202979/149‑1 (MQ=255)
                                                                                   aCCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGt                                                                                                     >  5:20181/1‑105 (MQ=255)
                                                                                   aCCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGt                                                                                                     <  6:20181/105‑1 (MQ=255)
                                                                                       gAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGAtt                                                             >  2:173937/1‑141 (MQ=255)
                                                                                       gAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCt                                                     >  2:242902/1‑149 (MQ=255)
                                                                                       gAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGCGGATCCAGCCGGCCCAGTCGCGGCGAtt                                                             <  1:173937/141‑1 (MQ=255)
                                                                                          cTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCACGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTgg                                                   >  5:18734/1‑148 (MQ=255)
                                                                                          cTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCACGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTgg                                                   <  6:18734/148‑1 (MQ=255)
                                                                                           tCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAgg                                                                                             <  5:78427/105‑1 (MQ=255)
                                                                                           tCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAgg                                                                                             >  6:78427/1‑105 (MQ=255)
                                                                                           tCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGc                                                           >  5:156228/1‑139 (MQ=255)
                                                                                           tCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGc                                                           <  6:156228/139‑1 (MQ=255)
                                                                                           tCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGc                                                  >  3:224041/1‑148 (MQ=255)
                                                                                           tCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGc                                                  >  8:242568/1‑148 (MQ=255)
                                                                                               tttCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACg                                             <  5:145238/149‑1 (MQ=255)
                                                                                               tttCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTAAGCAAACGCTGCATCAAGTGGCTCAGGGTGGGGAACCAGCCGACCCAAACGCGACAATTGCAGAGCTGCCCaaag                                             >  8:192050/1‑147 (MQ=255)
                                                                                                ttCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACgg                                            >  2:301284/1‑149 (MQ=255)
                                                                                                 tCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGcc                                                                           <  7:168631/117‑1 (MQ=255)
                                                                                                 tCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGcc                                                                           >  8:168631/1‑117 (MQ=255)
                                                                                                        ggTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGctc                                     >  6:197600/1‑148 (MQ=255)
                                                                                                               aCGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGCTCTATCAGtt                             <  8:112125/149‑1 (MQ=255)
                                                                                                                 gCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGCTCTATCAGtt                             >  1:301852/1‑147 (MQ=255)
                                                                                                                 gCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGCTCTATCAGTTGa                           <  7:242568/149‑1 (MQ=255)
                                                                                                                      tGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGCTCTATCAGt                              >  1:31618/1‑141 (MQ=255)
                                                                                                                      tGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGCTCTATCAGt                              <  2:31618/141‑1 (MQ=255)
                                                                                                                         cccAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGt                                                                                           >  8:117346/1‑77 (MQ=255)
                                                                                                                         cccAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGt                                                                                           <  7:117346/77‑1 (MQ=255)
                                                                                                                               gTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGCTCTATCAGTTGAGGGTTAGCGCTGAc             <  2:301852/149‑1 (MQ=255)
                                                                                                                                ttACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGCTCTATCAGTTGAGGGTTAgcgc                 >  7:265281/1‑144 (MQ=255)
                                                                                                                                ttACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGCTCTATCAGTTGAGGGTTAgcgc                 <  8:265281/144‑1 (MQ=255)
                                                                                                                                  aCGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGCTCTATCAGTTGAGGGTTAGCGCTGACCt           <  6:105858/148‑1 (MQ=255)
                                                                                                                                    gCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGCTCTATCAGTTGAGGGTTAGCGCTGACCTCGc        <  7:268694/149‑1 (MQ=255)
                                                                                                                                       gttgccGCTGCCGGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGCTCTATCAGTTGAGGGTTAGCGCTGACCTCGCTg      >  4:83609/6‑148 (MQ=255)
                                                                                                                                          gTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGCTCTATCAGTTGAGGGTTAGCGCTGACCTCGCTGAAGc  <  4:224041/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
CGCAGCTGGGCGCGCCAGACCGGGGTGGGTGTGGCGTGCCACCAGGCGATCGAGTTGTTCTGCGTCGACCCGGTGGCTGGCGAACCAGAACTCCATTTCAATCTGGTAATGACGCAGCTGGCCCAGCGTTACGCTCTGGTCGCTGCCCGGCAGCGGCAACGCCTCGCTCAGCAAACGCTGCATCCAGTGGCTCAGGGTGGGGATCCAGCCGGCCCAGTCGCGGCGATTGCAGCGCTGGCCAACGGTACGCTCTATCAGTTGAGGGTTAGCGCTGACCTCGCTGAAGC  >  NC_002947/5300503‑5300789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑