Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 402,065 A→C 10.0% I154S (ATC→AGC)  PP_0335 ← glutathione S‑transferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947402,0650AC10.0% 135.0 / 8.0 50I154S (ATC→AGC) PP_0335glutathione S‑transferase family protein
Reads supporting (aligned to +/- strand):  ref base A (21/24);  new base C (2/3);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

TGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGCCGCATCCATGATC  >  NC_002947/401917‑402208
                                                                                                                                                    |                                                                                                                                               
tGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGgaga                                                                                                                                                 <  6:68299/149‑1 (MQ=255)
  tAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGAt                                                                                                                                                <  6:163572/148‑1 (MQ=255)
     cGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGc                                                                                                                                            <  7:170431/149‑1 (MQ=255)
          aTCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCgg                                                                                                                                       <  5:235690/149‑1 (MQ=255)
             gATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGCTGATCTCCGGctt                                                                                                                                    <  2:289747/149‑3 (MQ=255)
             gATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGCTGATCTCCGGct                                                                                                                                     <  6:153068/148‑2 (MQ=255)
              aTGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAg                                                                                                                                   >  6:289035/1‑149 (MQ=255)
                  ccGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtgct                                                                                                                               >  5:96966/1‑149 (MQ=255)
                        gACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtgctcctgc                                                                                                                          <  5:289035/148‑1 (MQ=255)
                        gACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtgctcctg                                                                                                                           <  3:39559/147‑1 (MQ=255)
                           caccTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGCTGATCTCCGGctt                                                                                                                                    <  2:59612/135‑3 (MQ=255)
                           caccTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGCTGATCTCCGGctt                                                                                                                                    >  1:59612/1‑133 (MQ=255)
                                    aTACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTc                                                                                                               >  8:114205/1‑147 (MQ=255)
                                        cACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGc                                                                                                          >  4:199711/1‑148 (MQ=255)
                                         aCGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAACCCCGGGCTGACGCAAATCCAGCTAGCCCAGCGAACAGGCCACGCCAAGCGCCGCCAGCTCGAAGACGGCGATCAGCTCCGGCAAGTGCTACTGCTCACGGTTGTCAAGGCTa                                                                                                        >  3:21151/1‑149 (MQ=255)
                                         aCGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTa                                                                                                        >  7:77138/1‑149 (MQ=255)
                                          cgccgcCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTAc                                                                                                       >  1:142789/1‑149 (MQ=255)
                                                 aaCCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGAt                                                                                                >  5:5716/1‑149 (MQ=255)
                                                    ccAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCtt                                                                                             >  6:185906/1‑149 (MQ=255)
                                                          tGGCGCTCACGCCAGCCAAACCCGGGCTGACCCAAATCCAGATAGCCCAGCGCACATGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCTGGCTACGGCGGATCTTGTCAcc                                                                                       <  8:131579/149‑1 (MQ=255)
                                                            gcgcTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCt                                                                                     <  3:199711/149‑1 (MQ=255)
                                                               cTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTgcg                                                                                  >  6:156932/1‑149 (MQ=255)
                                                                tCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTgcgc                                                                                 >  7:249226/1‑149 (MQ=255)
                                                                  aCGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCtt                                                                               >  4:222512/1‑149 (MQ=255)
                                                                    gccagccaAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTg                                                                              >  4:267738/1‑148 (MQ=255)
                                                                       agccaAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACCCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGcagc                                                                          <  8:77138/149‑1 (MQ=255)
                                                                               ccGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCa                                                                                                             <  4:171656/106‑1 (MQ=255)
                                                                               ccGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCa                                                                                                             >  3:171656/1‑106 (MQ=255)
                                                                               ccGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATc                                                                  >  7:109140/1‑149 (MQ=255)
                                                                                cGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGGTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATcc                                                                 <  4:182964/149‑1 (MQ=255)
                                                                                             aTCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCct                                                    <  8:249226/149‑1 (MQ=255)
                                                                                              tCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCtt                                                                                             <  6:171767/107‑1 (MQ=255)
                                                                                              tCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCtt                                                                                             >  5:171767/1‑107 (MQ=255)
                                                                                              tCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCctc                                                   <  3:222512/149‑1 (MQ=255)
                                                                                                cAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTg                                                 <  3:267738/149‑1 (MQ=255)
                                                                                                      gCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCa                                           <  7:114205/149‑1 (MQ=255)
                                                                                                       cccAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAgccagcca                                                                    >  1:130020/1‑123 (MQ=255)
                                                                                                       cccAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAgccagcca                                                                    <  2:130020/123‑1 (MQ=255)
                                                                                                          aGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGaa                                       <  6:5716/149‑1 (MQ=255)
                                                                                                                caGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGctct                                  >  8:213561/1‑148 (MQ=255)
                                                                                                                   gCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATa                              >  1:115066/1‑149 (MQ=255)
                                                                                                                     cACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATAcc                            >  4:143512/1‑149 (MQ=255)
                                                                                                                       cGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCgg                          <  5:156932/149‑1 (MQ=255)
                                                                                                                               gccgccAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGaca                   <  7:213561/148‑1 (MQ=255)
                                                                                                                                 ctcaagttCGAAGCCTGAGAGCAGCTCCGGCAAGTGCTCCTGATCCAGGTTGTCAAGGCTACTGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTg                <  4:21151/142‑1 (MQ=255)
                                                                                                                                   ccAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGcc              >  4:218320/1‑149 (MQ=255)
                                                                                                                                   ccAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGcc              <  2:115066/149‑1 (MQ=255)
                                                                                                                                      ggTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGCCGCa           <  6:96966/149‑1 (MQ=255)
                                                                                                                                       gTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGCCGCAt          <  2:142789/149‑1 (MQ=255)
                                                                                                                                           ttgCCGGAGCTGATCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGCCGCATCCAt      >  3:92649/3‑149 (MQ=255)
                                                                                                                                               cGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGCCGCATCCATGATc  <  5:185906/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGCCGCATCCATGATC  >  NC_002947/401917‑402208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑