Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,319,175 T→G 11.3% K67Q (AAG→CAG) ‡ ilvI ← acetohydroxybutanoate synthase/acetolactate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,319,1750TG11.3% 145.5 / 8.6 52K67Q (AAG→CAG) ‡ilvIacetohydroxybutanoate synthase/acetolactate synthase
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base G (2/4);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCAGGAGAGCACCACCAGGGTACCCGTAGATGTGC  >  NC_002947/5319032‑5319314
                                                                                                                                               |                                                                                                                                           
cTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCgg                                                                                                                                        >  6:24592/1‑149 (MQ=255)
   gtaGGCATCGGTGGCCTGCTTGTTGTTTGGCACGTGGCCGGACAGGATGTCCATCGGTTTCGAGTCCATGTAGGCGGTGGCAATTCCGGTATTGGCTTTGGTCGCGCCAGGACCGGAGGTTTCCAGCACCACACCGGCATGTCCGGTg                                                                                                                                      <  2:65408/146‑1 (MQ=255)
   gAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGt                                                                                                                                       <  5:24592/147‑1 (MQ=255)
   gAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGAAGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGCAGGTTACCAGCACCACACCGGCCTTGCCGGTgg                                                                                                                                     >  8:239182/1‑149 (MQ=255)
    aaGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCAAGGCCGGTgtg                                                                                                                                    <  8:102359/149‑3 (MQ=255)
      ggCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGgcgc                                                                                                                                  >  7:58711/1‑149 (MQ=255)
         aTCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAg                                                                                                                               <  8:217584/149‑1 (MQ=255)
          tCGGTGCCCACCATGGTGTTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCAAGGCCGGTgt                                                                                                                                     <  3:262611/142‑2 (MQ=255)
                cccACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGcc                                                                                                                        <  5:167410/149‑1 (MQ=255)
                cccACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGcc                                                                                                                        >  4:133539/1‑149 (MQ=255)
                           gCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCatat                                                                                                             >  8:284432/1‑149 (MQ=255)
                             tAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGcc                                                                                                                        >  3:135976/1‑136 (MQ=255)
                             tAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGcc                                                                                                                        <  4:135976/136‑1 (MQ=255)
                             tAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATgg                                                                                                           >  8:204394/1‑149 (MQ=255)
                             tAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACAACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATgg                                                                                                           >  4:240757/1‑149 (MQ=255)
                               ggCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATgg                                                                                                           <  7:683/147‑1 (MQ=255)
                                       gCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGgccgc                                                                                                   <  5:207826/147‑1 (MQ=255)
                                          ggACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCt                                                                                              >  2:168593/1‑149 (MQ=255)
                                           gACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTc                                                                                             <  7:204394/149‑1 (MQ=255)
                                                gATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGAc                                                                                        >  8:275655/1‑149 (MQ=255)
                                                            aaTCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGAtgt                                                                            >  4:216714/1‑149 (MQ=255)
                                                            aaTCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGAtgt                                                                            >  7:96947/1‑149 (MQ=255)
                                                            aaTCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGAtgt                                                                            >  2:306476/1‑149 (MQ=255)
                                                                        gTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCt                                                                                              <  1:45419/119‑1 (MQ=255)
                                                                        gTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCt                                                                                              >  2:45419/1‑119 (MQ=255)
                                                                         tAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCgg                                                               <  3:240757/149‑1 (MQ=255)
                                                                           ggCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGt                                                              <  7:284432/148‑1 (MQ=255)
                                                                              ggTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCtt                                                          <  8:58711/149‑1 (MQ=255)
                                                                               gTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCttt                                                         <  3:216714/149‑1 (MQ=255)
                                                                               gTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCttt                                                         <  8:278873/149‑1 (MQ=255)
                                                                                  gCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGaa                                                      <  3:133539/149‑1 (MQ=255)
                                                                                        ccGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGc                                                <  5:150709/149‑1 (MQ=255)
                                                                                          ggTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGcgt                                              <  1:168593/149‑1 (MQ=255)
                                                                                           gTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGcgtc                                             <  1:306476/149‑1 (MQ=255)
                                                                                              aTGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGcgtcgt                                           <  7:275655/148‑1 (MQ=255)
                                                                                                 gCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTaaa                                        >  7:179383/1‑148 (MQ=255)
                                                                                                       gTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCa                                  <  7:239182/148‑1 (MQ=255)
                                                                                                              cAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGTTTGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCAGTAGAGca                          <  7:196114/149‑1 (MQ=255)
                                                                                                              cAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCAGGAGAGca                          <  7:159275/149‑1 (MQ=255)
                                                                                                              cAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCAGGAGAGca                          >  7:73309/1‑149 (MQ=255)
                                                                                                                    cGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCAGGAGAGcaccac                      >  1:288824/1‑147 (MQ=255)
                                                                                                                      gAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCAGGAGAGCACCACCAgg                  >  8:281074/1‑149 (MQ=255)
                                                                                                                              cAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTg                                                        <  1:105359/103‑1 (MQ=255)
                                                                                                                              cAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTg                                                        >  2:105359/1‑103 (MQ=255)
                                                                                                                              cAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCAGGAGAGCACCACCAGGGTACCCg           >  8:259080/1‑148 (MQ=255)
                                                                                                                                gCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCAGGAGAGCACCACCAGGGTACCCg           >  3:117626/1‑146 (MQ=255)
                                                                                                                                   ccacACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCAGGAGAGCACCACCAGGGTACCCGTAGAtg     >  7:109342/1‑149 (MQ=255)
                                                                                                                                    gccacCGGCAAGGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCAGgaga                             >  3:49641/3‑124 (MQ=255)
                                                                                                                                    gccacCGGCAAGGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCAGgaga                             <  4:49641/122‑1 (MQ=255)
                                                                                                                                    gccacCGGCAAGGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCAGGAGAGCACCACCAGGGTACCCGTAGAtgt    >  1:150305/3‑149 (MQ=255)
                                                                                                                                    cacaCCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTGGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGTACAGTGCGTCGTAAACATGCAGGAGAGCACCACCAGGGTACCCGTAGAtgt    >  7:157292/1‑149 (MQ=255)
                                                                                                                                      cacCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCAGGAGAGCACCACCAGGGTACCCGTAGATGTGc  <  8:96947/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                           
CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCGCCAGGACCGGAGGTTACCAGCACCACACCGGCCTTGCCGGTGGCGCGAGCGTAGCCGTCCGCCATATGGGTGGCCGCCTGCTCGTGACGAACCAGGATGTGTTCCACTTCCGGTTCTTTGAACAGTGCGTCGTAAACATGCAGGAGAGCACCACCAGGGTACCCGTAGATGTGC  >  NC_002947/5319032‑5319314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑