Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,391,329 A→G 7.5% E82E (GAA→GAG)  PP_5707 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,391,3290AG 7.5% 188.8 / 4.5 66E82E (GAA→GAG) PP_5707hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/29);  new base G (2/3);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

TTGCCAAAACGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCGCCATCAAC  >  NC_002947/5391182‑5391477
                                                                                                                                                   |                                                                                                                                                    
ttGCCAAAACGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTCATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGaa                                                                                                                                                      >  7:102334/1‑148 (MQ=255)
    cAAAACGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCgg                                                                                                                                                 >  6:56842/1‑149 (MQ=255)
      aaaCGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCa                                                                                                                                               <  5:56842/149‑1 (MQ=255)
      aaaCGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCa                                                                                                                                               <  8:8664/149‑1 (MQ=255)
       aaCGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCat                                                                                                                                              <  1:298755/149‑1 (MQ=255)
         cGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCatga                                                                                                                                            <  3:51031/149‑1 (MQ=255)
           cccGCGAACAGGCCTCGGAACTGATGGGCTATGCGCAGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAGTTCGGcatcatc                                                                                                                                          <  1:94257/149‑2 (MQ=255)
              gcgAACAGGCCTCGGAACTTATGGGCTATGCGCTGGACCTGACCATCGACGGACTGATGTAAGACAAGCCCTATCTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGGTTCTGGATGATGCCGAACTCGGCATGATGCGt                                                                                                                                       <  4:102410/149‑1 (MQ=255)
                    aGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAAt                                                                                                                                  >  1:4761/1‑148 (MQ=255)
                    aGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAAt                                                                                                                                  >  6:100550/1‑148 (MQ=255)
                    aGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATc                                                                                                                                 <  4:264200/149‑1 (MQ=255)
                        cTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACg                                                                                                                              >  5:252529/1‑148 (MQ=255)
                         tCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAGTTCGGcatcatcca                                                                                                                                        <  6:118349/137‑4 (MQ=255)
                         tCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAGTTCGGcatcatcca                                                                                                                                        >  5:118349/1‑134 (MQ=255)
                               cTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGAACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCTGAATGATGCGTTGAATCACGCACCGCGa                                                                                                                      >  3:188005/1‑149 (MQ=255)
                                tGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGAt                                                                                                                     >  8:98112/1‑149 (MQ=255)
                                   tGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGc                                                                                                                   <  6:18023/148‑1 (MQ=255)
                                      gCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTaa                                                                                                               <  1:135736/149‑1 (MQ=255)
                                         aTGCGCGGGACCTGACCATCGACGGACTGATGAAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAgg                                                                                                            >  1:193225/1‑149 (MQ=255)
                                          tGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAggg                                                                                                           <  5:9510/149‑1 (MQ=255)
                                            cgcgGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAgggg                                                                                                          <  4:270913/148‑1 (MQ=255)
                                             gcgGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGt                                                                                                         >  2:97454/1‑148 (MQ=255)
                                                  aCCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGt                                                                                                   <  2:4761/149‑1 (MQ=255)
                                                   ccTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCTGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGt                                                                                                   >  4:214763/1‑148 (MQ=255)
                                                   ccTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGCCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTc                                                                                                  <  1:97454/149‑1 (MQ=255)
                                                       aCCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGcc                                                                                              >  5:223376/1‑149 (MQ=255)
                                                         cATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGc                                                                                            >  5:127154/1‑149 (MQ=255)
                                                           tCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCtt                                                                                          >  4:107381/1‑149 (MQ=255)
                                                                ggACTGATGGAAGACAAGCCCAATCTGATCTTGGCGGCGCACTACCTGTGTGCTTTGGGCAAAGCGCTACTGTATGATGCCGAACTCGGCATGATGCGTTGAATGACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGccc                                                                                     <  4:188005/149‑1 (MQ=255)
                                                                 gACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGcccc                                                                                    <  5:100550/149‑1 (MQ=255)
                                                                  aCTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCt                                                                                   >  8:122729/1‑149 (MQ=255)
                                                                      aTGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTTCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATTCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTa                                                                               <  3:61197/149‑1 (MQ=255)
                                                                        ggAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACg                                                                             >  3:184396/1‑149 (MQ=255)
                                                                        ggAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACg                                                                             >  6:8435/1‑149 (MQ=255)
                                                                        ggAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACg                                                                             >  7:43484/1‑149 (MQ=255)
                                                                          aaGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGcc                                                                           <  2:193225/149‑1 (MQ=255)
                                                                          aaGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGcc                                                                           <  7:208809/149‑1 (MQ=255)
                                                                                aGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGa                                                                     >  6:173071/1‑149 (MQ=255)
                                                                                  cccAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGAt                                                                    >  3:128545/1‑148 (MQ=255)
                                                                                     aaaCTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAAc                                                                <  6:223376/149‑1 (MQ=255)
                                                                                      aaCTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACg                                                               >  4:102590/1‑149 (MQ=255)
                                                                                         tGATCTGGCCGGAGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCCTCAGACGCTTGGCCCCATTTACGCAACACGATCAACGcc                                                             >  8:156471/1‑148 (MQ=255)
                                                                                            tCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACACCACACGATCAACGCCGAgg                                                         >  3:157317/1‑149 (MQ=255)
                                                                                            tCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGAGCTACTGGATGATGCAGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAgg                                                         >  7:227988/1‑149 (MQ=255)
                                                                                               gggCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACCCCACACCATCAACCCCGAgggtt                                                      >  5:199950/1‑147 (MQ=255)
                                                                                                ggcggcGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGAt                                                      <  6:252529/148‑1 (MQ=255)
                                                                                                        aCTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCttt                                             <  6:127154/149‑1 (MQ=255)
                                                                                                         cTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTtc                                            <  3:107381/149‑1 (MQ=255)
                                                                                                         cTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTtc                                            >  4:45598/1‑149 (MQ=255)
                                                                                                            ccTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTtctc                                          <  3:45598/148‑1 (MQ=255)
                                                                                                            ccTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTtctc                                          <  7:122729/148‑1 (MQ=255)
                                                                                                               ctGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGAt                                                                                                                     <  6:253346/70‑1 (MQ=255)
                                                                                                               ctGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGAt                                                                                                                     >  5:253346/1‑70 (MQ=255)
                                                                                                                     tttGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTc                                >  2:205810/1‑149 (MQ=255)
                                                                                                                        ggtcAAAGAGCTACTGGATGATGCCGAACTCGTCATGATGCGTTGAATCACGCACCGCTATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGc                              <  7:156471/145‑1 (MQ=255)
                                                                                                                         ggCAAAGCGCTACTGGATGATGCCGAACTCGTCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCAt                            >  6:14621/1‑149 (MQ=255)
                                                                                                                           cAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCAtt                           >  8:84859/1‑148 (MQ=255)
                                                                                                                               gcgcTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATgc                      <  8:43484/149‑1 (MQ=255)
                                                                                                                                        catcatgCCGAGTTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACTCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCgcagc              >  3:188010/5‑148 (MQ=255)
                                                                                                                                          tgatgCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTgg                                                                                        <  3:77004/72‑1 (MQ=255)
                                                                                                                                          tgatgCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTgg                                                                                        >  4:77004/1‑72 (MQ=255)
                                                                                                                                            atgCCGAGTTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCg          <  4:188010/148‑1 (MQ=255)
                                                                                                                                               ccGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCGCCAt      <  3:102590/149‑1 (MQ=255)
                                                                                                                                               ccGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCGCCAt      >  2:40256/1‑149 (MQ=255)
                                                                                                                                               ccGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACACCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGAAGCATCGCCa       <  4:157317/148‑1 (MQ=255)
                                                                                                                                                cGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCGCCAt      >  6:263799/1‑148 (MQ=255)
                                                                                                                                                 gAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCGCCATc     >  2:79907/1‑148 (MQ=255)
                                                                                                                                                   aCTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCGCCATCAAc  <  5:8435/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTGCCAAAACGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCGCCATCAAC  >  NC_002947/5391182‑5391477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑