Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,391,330 C→T 7.4% L83F (CTC→TTC)  PP_5707 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,391,3300CT 7.4% 195.6 / 4.9 67L83F (CTC→TTC) PP_5707hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/30);  new base T (2/3);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

CAAAACGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCGCCATCAAC  >  NC_002947/5391186‑5391477
                                                                                                                                                |                                                                                                                                                   
cAAAACGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCgg                                                                                                                                                 >  6:56842/1‑149 (MQ=255)
  aaaCGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCa                                                                                                                                               <  8:8664/149‑1 (MQ=255)
  aaaCGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCa                                                                                                                                               <  5:56842/149‑1 (MQ=255)
   aaCGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCat                                                                                                                                              <  1:298755/149‑1 (MQ=255)
     cGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCatga                                                                                                                                            <  3:51031/149‑1 (MQ=255)
       cccGCGAACAGGCCTCGGAACTGATGGGCTATGCGCAGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAGTTCGGcatcatc                                                                                                                                          <  1:94257/149‑2 (MQ=255)
          gcgAACAGGCCTCGGAACTTATGGGCTATGCGCTGGACCTGACCATCGACGGACTGATGTAAGACAAGCCCTATCTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGGTTCTGGATGATGCCGAACTCGGCATGATGCGt                                                                                                                                       <  4:102410/149‑1 (MQ=255)
                aGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAAt                                                                                                                                  >  1:4761/1‑148 (MQ=255)
                aGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAAt                                                                                                                                  >  6:100550/1‑148 (MQ=255)
                aGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATc                                                                                                                                 <  4:264200/149‑1 (MQ=255)
                    cTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACg                                                                                                                              >  5:252529/1‑148 (MQ=255)
                     tCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAGTTCGGcatcatcca                                                                                                                                        <  6:118349/137‑4 (MQ=255)
                     tCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAGTTCGGcatcatcca                                                                                                                                        >  5:118349/1‑134 (MQ=255)
                           cTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGAACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCTGAATGATGCGTTGAATCACGCACCGCGa                                                                                                                      >  3:188005/1‑149 (MQ=255)
                            tGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGAt                                                                                                                     >  8:98112/1‑149 (MQ=255)
                               tGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGc                                                                                                                   <  6:18023/148‑1 (MQ=255)
                                  gCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTaa                                                                                                               <  1:135736/149‑1 (MQ=255)
                                     aTGCGCGGGACCTGACCATCGACGGACTGATGAAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAgg                                                                                                            >  1:193225/1‑149 (MQ=255)
                                      tGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAggg                                                                                                           <  5:9510/149‑1 (MQ=255)
                                        cgcgGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAgggg                                                                                                          <  4:270913/148‑1 (MQ=255)
                                         gcgGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGt                                                                                                         >  2:97454/1‑148 (MQ=255)
                                              aCCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGt                                                                                                   <  2:4761/149‑1 (MQ=255)
                                               ccTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCTGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGt                                                                                                   >  4:214763/1‑148 (MQ=255)
                                               ccTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGCCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTc                                                                                                  <  1:97454/149‑1 (MQ=255)
                                                   aCCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGcc                                                                                              >  5:223376/1‑149 (MQ=255)
                                                     cATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGc                                                                                            >  5:127154/1‑149 (MQ=255)
                                                       tCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCtt                                                                                          >  4:107381/1‑149 (MQ=255)
                                                            ggACTGATGGAAGACAAGCCCAATCTGATCTTGGCGGCGCACTACCTGTGTGCTTTGGGCAAAGCGCTACTGTATGATGCCGAACTCGGCATGATGCGTTGAATGACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGccc                                                                                     <  4:188005/149‑1 (MQ=255)
                                                             gACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGcccc                                                                                    <  5:100550/149‑1 (MQ=255)
                                                              aCTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCt                                                                                   >  8:122729/1‑149 (MQ=255)
                                                                  aTGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTTCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATTCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTa                                                                               <  3:61197/149‑1 (MQ=255)
                                                                    ggAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACg                                                                             >  7:43484/1‑149 (MQ=255)
                                                                    ggAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACg                                                                             >  3:184396/1‑149 (MQ=255)
                                                                    ggAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACg                                                                             >  6:8435/1‑149 (MQ=255)
                                                                      aaGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGcc                                                                           <  2:193225/149‑1 (MQ=255)
                                                                      aaGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGcc                                                                           <  7:208809/149‑1 (MQ=255)
                                                                            aGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGa                                                                     >  6:173071/1‑149 (MQ=255)
                                                                              cccAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGAt                                                                    >  3:128545/1‑148 (MQ=255)
                                                                                 aaaCTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAAc                                                                <  6:223376/149‑1 (MQ=255)
                                                                                  aaCTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACg                                                               >  4:102590/1‑149 (MQ=255)
                                                                                     tGATCTGGCCGGAGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCCTCAGACGCTTGGCCCCATTTACGCAACACGATCAACGcc                                                             >  8:156471/1‑148 (MQ=255)
                                                                                        tCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACACCACACGATCAACGCCGAgg                                                         >  3:157317/1‑149 (MQ=255)
                                                                                        tCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGAGCTACTGGATGATGCAGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAgg                                                         >  7:227988/1‑149 (MQ=255)
                                                                                           gggCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACCCCACACCATCAACCCCGAgggtt                                                      >  5:199950/1‑147 (MQ=255)
                                                                                            ggcggcGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGAt                                                      <  6:252529/148‑1 (MQ=255)
                                                                                                    aCTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCttt                                             <  6:127154/149‑1 (MQ=255)
                                                                                                     cTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTtc                                            <  3:107381/149‑1 (MQ=255)
                                                                                                     cTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTtc                                            >  4:45598/1‑149 (MQ=255)
                                                                                                        ccTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTtctc                                          <  7:122729/148‑1 (MQ=255)
                                                                                                        ccTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTtctc                                          <  3:45598/148‑1 (MQ=255)
                                                                                                           ctGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGAt                                                                                                                     >  5:253346/1‑70 (MQ=255)
                                                                                                           ctGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGAt                                                                                                                     <  6:253346/70‑1 (MQ=255)
                                                                                                                 tttGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTc                                >  2:205810/1‑149 (MQ=255)
                                                                                                                    ggtcAAAGAGCTACTGGATGATGCCGAACTCGTCATGATGCGTTGAATCACGCACCGCTATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGc                              <  7:156471/145‑1 (MQ=255)
                                                                                                                     ggCAAAGCGCTACTGGATGATGCCGAACTCGTCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCAt                            >  6:14621/1‑149 (MQ=255)
                                                                                                                       cAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCAtt                           >  8:84859/1‑148 (MQ=255)
                                                                                                                           gcgcTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATgc                      <  8:43484/149‑1 (MQ=255)
                                                                                                                                    catcatgCCGAGTTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACTCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCgcagc              >  3:188010/5‑148 (MQ=255)
                                                                                                                                      tgatgCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTgg                                                                                        >  4:77004/1‑72 (MQ=255)
                                                                                                                                      tgatgCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTgg                                                                                        <  3:77004/72‑1 (MQ=255)
                                                                                                                                        atgCCGAGTTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCg          <  4:188010/148‑1 (MQ=255)
                                                                                                                                           ccGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCGCCAt      >  2:40256/1‑149 (MQ=255)
                                                                                                                                           ccGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCGCCAt      <  3:102590/149‑1 (MQ=255)
                                                                                                                                           ccGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACACCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGAAGCATCGCCa       <  4:157317/148‑1 (MQ=255)
                                                                                                                                            cGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCGCCAt      >  6:263799/1‑148 (MQ=255)
                                                                                                                                             gAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCGCCATc     >  2:79907/1‑148 (MQ=255)
                                                                                                                                               aCTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCGCCATCAAc  <  5:8435/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CAAAACGCCCGCGAACAGGCCTCGGAACTGATGGGCTATGCGCGGGACCTGACCATCGACGGACTGATGGAAGACAAGCCCAAACTGATCTGGGCGGCGCACTACCTCTGTGCTTTGGGCAAAGCGCTACTGGATGATGCCGAACTCGGCATGATGCGTTGAATCACGCACCGCGATGCTTAAAGGGGTCATCGTCAGCCGCTTGGCCCCTTTTACGCCACACGATCAACGCCGAGGGATGCGGTCTTTCTCAGCAACCGTCGCATTTATGCGCCGCAGCATCGCCATCAAC  >  NC_002947/5391186‑5391477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑