Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,419,959 G→C 7.0% A215A (GCG→GCC)  PP_4759 → transcriptional regulator, GntR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,419,9590GC 7.0% 180.5 / 3.3 57A215A (GCG→GCC) PP_4759transcriptional regulator, GntR family
Reads supporting (aligned to +/- strand):  ref base G (22/31);  new base C (2/2);  total (24/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

GCTGCAACTGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCACCAGGTCAGGCAGA  >  NC_002947/5419812‑5420104
                                                                                                                                                   |                                                                                                                                                   
gctgcAACTGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCcgcgc                                                                                                                                                    >  2:9777/1‑149 (MQ=255)
  tgcAACTGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCgcgg                                                                                                                                                  >  8:156723/1‑149 (MQ=255)
    cAACTGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCgcgggc                                                                                                                                                >  8:87947/1‑149 (MQ=255)
        tGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCgcgggc‑gg                                                                                                                                             >  8:260594/1‑147 (MQ=255)
          ggTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCa                                                                                                                                           <  7:87947/147‑1 (MQ=255)
          ggTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAgt                                                                                                                                         <  4:136374/149‑1 (MQ=255)
          ggTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAgt                                                                                                                                         >  2:3904/1‑149 (MQ=255)
            tAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAgtgt                                                                                                                                       <  1:132251/149‑1 (MQ=255)
            tAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCCCGCGC‑GGCatccg                                                                                                                                       >  8:148458/1‑145 (MQ=255)
              aCGGCCTGCTCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCCCGCGC‑GGCatccgg                                                                                                                                      <  1:47928/148‑6 (MQ=255)
              aCGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGc                                                                                                                                     <  1:9777/149‑1 (MQ=255)
              aCGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGc                                                                                                                                     <  7:156723/149‑1 (MQ=255)
              aCGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCCCGCGC‑GGCatccgg                                                                                                                                      <  7:148458/148‑6 (MQ=255)
               cGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGcc                                                                                                                                    <  1:144219/149‑1 (MQ=255)
                ggCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCt                                                                                                                                   >  1:58460/1‑149 (MQ=255)
                 gCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTg                                                                                                                                  >  5:95745/1‑149 (MQ=255)
                 gCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTg                                                                                                                                  <  7:227927/149‑1 (MQ=255)
                      aTCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCAc                                                                                                                               <  8:184951/147‑1 (MQ=255)
                      aTCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCAc                                                                                                                               <  3:249192/147‑1 (MQ=255)
                              cAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTg                                                                                                                                  <  7:291803/136‑1 (MQ=255)
                              cAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTg                                                                                                                                  >  8:291803/1‑136 (MQ=255)
                                 ccGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGcc                                                                                                                 <  2:175921/149‑1 (MQ=255)
                                   gCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCga                                                                                                               <  2:72749/149‑1 (MQ=255)
                                         cccAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCgg                                                                                                         >  4:241850/1‑149 (MQ=255)
                                         cccAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCgg                                                                                                         <  4:239010/149‑1 (MQ=255)
                                          ccAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGc                                                                                                        <  7:123959/149‑1 (MQ=255)
                                                 gAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGcc                                                                                                 >  2:179016/1‑149 (MQ=255)
                                                     gcgcgGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGTCCCAACAGCCGAGACCGGCTACTGCCGga                                                                                              <  1:3904/149‑1 (MQ=255)
                                                     gcgcgGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGgac                                                                                             <  3:139576/149‑1 (MQ=255)
                                                      cgcgGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTAGAGCATGAGGCGATCCTCAATGCCATCCGCCGACATGACCCGGATGCCGCGCGGGC‑GGCAGTATGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGgact                                                                                            <  5:195871/149‑1 (MQ=255)
                                                        cgGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGgactga                                                                                          <  6:189683/149‑1 (MQ=255)
                                                          ggggCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGgactgact                                                                                        <  7:260594/149‑1 (MQ=255)
                                                             gCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGt                                                                                      >  1:190532/1‑148 (MQ=255)
                                                              ccAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGt                                                                                      >  4:103385/1‑147 (MQ=255)
                                                                  gCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCa                                                                                <  3:50897/149‑1 (MQ=255)
                                                                   cTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCTGACAGTCAGTCCGGCAg                                                                               <  3:34985/149‑1 (MQ=255)
                                                                               gCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGt                                                                   <  4:251715/149‑1 (MQ=255)
                                                                                     cTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCCAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTg                                                             >  6:223656/1‑149 (MQ=255)
                                                                                                ggAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAgtag                                                                            >  5:184109/1‑121 (MQ=255)
                                                                                                ggAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAgtag                                                                            >  5:188380/1‑121 (MQ=255)
                                                                                                ggAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAgtag                                                                            <  6:188380/123‑3 (MQ=255)
                                                                                                ggAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAgtag                                                                            <  6:184109/123‑3 (MQ=255)
                                                                                                ggAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGACTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGt                                                  >  5:157312/1‑149 (MQ=255)
                                                                                                         ggCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCa                                                      <  5:47352/136‑1 (MQ=255)
                                                                                                         ggCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCa                                                      >  6:47352/1‑136 (MQ=255)
                                                                                                              tCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATc                                      >  5:306045/1‑147 (MQ=255)
                                                                                                                 tCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGc                                  >  3:85375/1‑148 (MQ=255)
                                                                                                                        cATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTg                           >  1:197558/1‑149 (MQ=255)
                                                                                                                         aTCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGcc                         <  3:103385/149‑1 (MQ=255)
                                                                                                                                 aCAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGa                                                                     <  4:240785/97‑1 (MQ=255)
                                                                                                                                 aCAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGa                                                                     >  3:240785/1‑97 (MQ=255)
                                                                                                                                 aCAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACtt                  >  2:290714/1‑148 (MQ=255)
                                                                                                                                 aCAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTc                 <  2:190532/149‑1 (MQ=255)
                                                                                                                                   aaGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCg                                             >  1:258694/1‑119 (MQ=255)
                                                                                                                                   aaGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCg                                             <  2:258694/119‑1 (MQ=255)
                                                                                                                                    aGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCAcc              <  6:306045/149‑1 (MQ=255)
                                                                                                                                     gACCCGGATGCCGCGCGGGC‑GGCAGTGTCCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCAc               >  1:171975/1‑147 (MQ=255)
                                                                                                                                        ccGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCACCAGGt          >  1:118875/1‑149 (MQ=255)
                                                                                                                                        cacactGCCGCCCGCGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCACCAGGt          >  7:24154/6‑149 (MQ=255)
                                                                                                                                           gATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCACCAGGTCAg       >  5:185284/1‑149 (MQ=255)
                                                                                                                                             tGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCACCAGGTCAGGc     <  6:157312/149‑1 (MQ=255)
                                                                                                                                                cgcgcgGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCACCAGGTCAGGCAGa  <  4:145230/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCTGCAACTGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCACCAGGTCAGGCAGA  >  NC_002947/5419812‑5420104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑