Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,419,962 G→C 6.9% R216R (CGG→CGC)  PP_4759 → transcriptional regulator, GntR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,419,9620GC 6.9% 179.0 / 2.8 58R216R (CGG→CGC) PP_4759transcriptional regulator, GntR family
Reads supporting (aligned to +/- strand):  ref base G (22/32);  new base C (2/2);  total (24/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

TGCAACTGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCACCAGGTCAGGCAGA  >  NC_002947/5419814‑5420104
                                                                                                                                                    |                                                                                                                                                
tgcAACTGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCgcgg                                                                                                                                                  >  8:156723/1‑149 (MQ=255)
  cAACTGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCgcgggc                                                                                                                                                >  8:87947/1‑149 (MQ=255)
      tGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCgcgggc‑gg                                                                                                                                             >  8:260594/1‑147 (MQ=255)
        ggTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCa                                                                                                                                           <  7:87947/147‑1 (MQ=255)
        ggTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAgt                                                                                                                                         <  4:136374/149‑1 (MQ=255)
        ggTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAgt                                                                                                                                         >  2:3904/1‑149 (MQ=255)
          tAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAgtgt                                                                                                                                       <  1:132251/149‑1 (MQ=255)
          tAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCCCGCGC‑GGCatccg                                                                                                                                       >  8:148458/1‑145 (MQ=255)
            aCGGCCTGCTCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCCCGCGC‑GGCatccgg                                                                                                                                      <  1:47928/148‑6 (MQ=255)
            aCGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGc                                                                                                                                     <  7:156723/149‑1 (MQ=255)
            aCGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGc                                                                                                                                     <  1:9777/149‑1 (MQ=255)
            aCGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCCCGCGC‑GGCatccgg                                                                                                                                      <  7:148458/148‑6 (MQ=255)
             cGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGcc                                                                                                                                    <  1:144219/149‑1 (MQ=255)
              ggCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCt                                                                                                                                   >  1:58460/1‑149 (MQ=255)
               gCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTg                                                                                                                                  >  5:95745/1‑149 (MQ=255)
               gCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTg                                                                                                                                  <  7:227927/149‑1 (MQ=255)
                    aTCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCAc                                                                                                                               <  8:184951/147‑1 (MQ=255)
                    aTCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCAc                                                                                                                               <  3:249192/147‑1 (MQ=255)
                            cAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTg                                                                                                                                  <  7:291803/136‑1 (MQ=255)
                            cAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTg                                                                                                                                  >  8:291803/1‑136 (MQ=255)
                               ccGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGcc                                                                                                                 <  2:175921/149‑1 (MQ=255)
                                 gCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCga                                                                                                               <  2:72749/149‑1 (MQ=255)
                                       cccAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCgg                                                                                                         <  4:239010/149‑1 (MQ=255)
                                       cccAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCgg                                                                                                         >  4:241850/1‑149 (MQ=255)
                                        ccAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGc                                                                                                        <  7:123959/149‑1 (MQ=255)
                                               gAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGcc                                                                                                 >  2:179016/1‑149 (MQ=255)
                                                   gcgcgGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGTCCCAACAGCCGAGACCGGCTACTGCCGga                                                                                              <  1:3904/149‑1 (MQ=255)
                                                   gcgcgGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGgac                                                                                             <  3:139576/149‑1 (MQ=255)
                                                    cgcgGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTAGAGCATGAGGCGATCCTCAATGCCATCCGCCGACATGACCCGGATGCCGCGCGGGC‑GGCAGTATGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGgact                                                                                            <  5:195871/149‑1 (MQ=255)
                                                      cgGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGgactga                                                                                          <  6:189683/149‑1 (MQ=255)
                                                        ggggCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGgactgact                                                                                        <  7:260594/149‑1 (MQ=255)
                                                           gCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGt                                                                                      >  1:190532/1‑148 (MQ=255)
                                                            ccAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGt                                                                                      >  4:103385/1‑147 (MQ=255)
                                                                gCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCa                                                                                <  3:50897/149‑1 (MQ=255)
                                                                 cTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCTGACAGTCAGTCCGGCAg                                                                               <  3:34985/149‑1 (MQ=255)
                                                                             gCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGt                                                                   <  4:251715/149‑1 (MQ=255)
                                                                                   cTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCCAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTg                                                             >  6:223656/1‑149 (MQ=255)
                                                                                              ggAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAgtag                                                                            >  5:188380/1‑121 (MQ=255)
                                                                                              ggAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAgtag                                                                            >  5:184109/1‑121 (MQ=255)
                                                                                              ggAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAgtag                                                                            <  6:188380/123‑3 (MQ=255)
                                                                                              ggAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAgtag                                                                            <  6:184109/123‑3 (MQ=255)
                                                                                              ggAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGACTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGt                                                  >  5:157312/1‑149 (MQ=255)
                                                                                                       ggCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCa                                                      <  5:47352/136‑1 (MQ=255)
                                                                                                       ggCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCa                                                      >  6:47352/1‑136 (MQ=255)
                                                                                                            tCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATc                                      >  5:306045/1‑147 (MQ=255)
                                                                                                               tCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGc                                  >  3:85375/1‑148 (MQ=255)
                                                                                                                      cATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTg                           >  1:197558/1‑149 (MQ=255)
                                                                                                                       aTCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGcc                         <  3:103385/149‑1 (MQ=255)
                                                                                                                               aCAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGa                                                                     <  4:240785/97‑1 (MQ=255)
                                                                                                                               aCAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGa                                                                     >  3:240785/1‑97 (MQ=255)
                                                                                                                               aCAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACtt                  >  2:290714/1‑148 (MQ=255)
                                                                                                                               aCAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTc                 <  2:190532/149‑1 (MQ=255)
                                                                                                                                 aaGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCg                                             >  1:258694/1‑119 (MQ=255)
                                                                                                                                 aaGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCg                                             <  2:258694/119‑1 (MQ=255)
                                                                                                                                  aGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCAcc              <  6:306045/149‑1 (MQ=255)
                                                                                                                                   gACCCGGATGCCGCGCGGGC‑GGCAGTGTCCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCAc               >  1:171975/1‑147 (MQ=255)
                                                                                                                                      ccGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCACCAGGt          >  1:118875/1‑149 (MQ=255)
                                                                                                                                      cacactGCCGCCCGCGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCACCAGGt          >  7:24154/6‑149 (MQ=255)
                                                                                                                                         gATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCACCAGGTCAg       >  5:185284/1‑149 (MQ=255)
                                                                                                                                           tGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCACCAGGTCAGGc     <  6:157312/149‑1 (MQ=255)
                                                                                                                                              cgcgcgGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCACCAGGTCAGGCAGa  <  4:145230/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TGCAACTGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGC‑GGCAGTGTGCCTGCACCTGT‑CCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCACTTCACCAGGTCAGGCAGA  >  NC_002947/5419814‑5420104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑