Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,420,450 A→G 12.3% W217R (TGG→CGG)  PP_4760 ← alcohol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,420,4500AG12.3% 117.7 / 8.3 49W217R (TGG→CGG) PP_4760alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (19/23);  new base G (3/3);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

ACCCCGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACACCGCCGGTA  >  NC_002947/5420308‑5420595
                                                                                                                                              |                                                                                                                                                 
aCCCCGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCgg                                                                                                                                             <  5:187471/149‑1 (MQ=255)
 ccccGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCggg                                                                                                                                            <  6:153683/149‑1 (MQ=255)
 ccccGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCggg                                                                                                                                            <  3:106758/149‑1 (MQ=255)
  cccGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCgggg                                                                                                                                           <  8:187767/149‑1 (MQ=255)
     ggTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGc                                                                                                                                        >  3:222520/1‑149 (MQ=255)
         aGGATGCCTATCAGAGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCGGCCTGGGGTgagaag                                                                                                                                    <  8:232892/149‑6 (MQ=255)
         aGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGGACCTTCTCACCCCGGCCTGGGGTgagaa                                                                                                                                     <  1:8938/148‑5 (MQ=255)
         aGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCGGCCTGGGGTgagaag                                                                                                                                    <  5:293335/149‑6 (MQ=255)
             tGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAg                                                                                                                                <  4:222520/149‑1 (MQ=255)
                cGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTg                                                                                                                             <  8:86607/149‑1 (MQ=255)
                cGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTg                                                                                                                             >  3:159075/1‑149 (MQ=255)
                   tCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATc                                                                                                                          <  8:153218/149‑1 (MQ=255)
                     aGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAg                                                                                                                        <  3:193119/149‑1 (MQ=255)
                       cGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCggg                                                                                                                                            >  1:267621/1‑127 (MQ=255)
                       cGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCggg                                                                                                                                            <  2:267621/127‑1 (MQ=255)
                       cGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCggg                                                                                                                                            <  6:87373/127‑1 (MQ=255)
                       cGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCggg                                                                                                                                            >  5:87373/1‑127 (MQ=255)
                        gAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAggtg                                                                                                                     <  5:109533/149‑1 (MQ=255)
                           aCATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTc                                                                                                                  <  5:245580/149‑1 (MQ=255)
                             aTGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCgg                                                                                                                >  7:12158/1‑149 (MQ=255)
                                cccGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCa                                                                                                              <  4:159075/148‑1 (MQ=255)
                                     cAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAgg                                                                                                        >  8:133726/1‑149 (MQ=255)
                                          cgcgcAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCtt                                                                                                   <  1:5962/149‑1 (MQ=255)
                                           gcgcAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCtt                                                                                                   >  4:137883/1‑148 (MQ=255)
                                             gcagcaATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAg                                                                                                <  6:75536/149‑1 (MQ=255)
                                                  aaTCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTGAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTGGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCt                                                                                           >  3:105512/1‑149 (MQ=255)
                                                         gACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCtt                                                                                    <  3:137883/149‑1 (MQ=255)
                                                               tCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATc                                                                              >  8:70729/1‑149 (MQ=255)
                                                                ccAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCg                                                                             >  4:218316/1‑149 (MQ=255)
                                                                             gccgccCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCCGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTgg                                                                <  5:261296/149‑1 (MQ=255)
                                                                              ccgcaCACTTCAATCACATGATCGAAACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTTGTAGGCACCCAGGGCCTTAAGGCTCTACAGCTTGGCATCGCTGGACGAGGTCGc                                                               >  8:52386/1‑149 (MQ=255)
                                                                                  ccACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCAccc                                                                                                           >  5:92184/1‑101 (MQ=255)
                                                                                  ccACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCAccc                                                                                                           <  6:92184/101‑1 (MQ=255)
                                                                                   cACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGATGAGGTGGCGATCa                                                          <  7:70729/149‑1 (MQ=255)
                                                                                                tGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGcgcccgc                                             <  3:218316/149‑1 (MQ=255)
                                                                                                          cacGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGc                                    >  8:166005/1‑148 (MQ=255)
                                                                                                                   cAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAgg                          >  2:50878/1‑149 (MQ=255)
                                                                                                                        aGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGaa                     <  8:245317/149‑1 (MQ=255)
                                                                                                                        aGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTAGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGa                      >  7:158828/1‑148 (MQ=255)
                                                                                                                         gCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGaa                     >  2:307280/1‑148 (MQ=255)
                                                                                                                             gcgcACCTTCTCACCCCACGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATc                 <  8:12158/148‑1 (MQ=255)
                                                                                                                              cgcACCTTCTCACCCCAGTCCGGGGTGCTCTTGTATTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTTGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATcta               <  7:52386/149‑3 (MQ=255)
                                                                                                                                     gagcACCCCGGCCTGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACAccg        >  4:142555/4‑149 (MQ=255)
                                                                                                                                     gagcACCCCGGCCTGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACAccg        >  2:272032/4‑149 (MQ=255)
                                                                                                                                      agcACCCCGGCCTGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACAccgc       >  7:54453/3‑149 (MQ=255)
                                                                                                                                         aCCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTg                                                                          <  5:288034/79‑1 (MQ=255)
                                                                                                                                         aCCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTg                                                                          >  6:288034/1‑79 (MQ=255)
                                                                                                                                           cccAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACACCGCCGGTa  >  8:157102/1‑149 (MQ=255)
                                                                                                                                           cccAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACACCGCCGGTa  >  6:23231/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
ACCCCGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACACCGCCGGTA  >  NC_002947/5420308‑5420595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑