Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,472,538 A→G 8.1% M94T (ATG→ACG)  rsfS ← ribosomal silencing factor RsfS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,472,5380AG 8.1% 134.4 / 2.7 49M94T (ATG→ACG) rsfSribosomal silencing factor RsfS
Reads supporting (aligned to +/- strand):  ref base A (22/23);  new base G (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

CCTTATTCGCGGTCTTTGAGCTTGTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTACCGGTG  >  NC_002947/5472397‑5472684
                                                                                                                                             |                                                                                                                                                  
ccTTATTCGCGGTCTTTGAGCTTGTCGGAGTACTCGTTGGCATGGTCCGGGCTTTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCa                                                                                                                                              <  8:293304/148‑1 (MQ=255)
    aTTCGCGGTCTTTGAGCTTGTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGAt                                                                                                                                          <  2:23334/148‑1 (MQ=255)
              tttGAGCTTGTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGtt                                                                                                                                 >  1:286960/1‑147 (MQ=255)
                tGAGCTTGTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAg                                                                                                                              <  2:102772/148‑1 (MQ=255)
                      tGTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGTCATCGGCGGAACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGAAGCGGCGGTCATCATGTGCACGATGACGTCGTAGAGGTCcag                                                                                                                        >  4:44979/1‑148 (MQ=255)
                       gTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCcagc                                                                                                                       <  8:212650/148‑1 (MQ=255)
                       gTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCcag                                                                                                                        <  3:129302/147‑1 (MQ=255)
                       gTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCcag                                                                                                                        <  6:33321/147‑1 (MQ=255)
                                 tCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTc                                                                                                            >  3:230198/1‑149 (MQ=255)
                                         aTGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGc                                                                                                    >  7:43625/1‑149 (MQ=255)
                                          tGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGcc                                                                                                   <  2:142825/149‑1 (MQ=255)
                                            gTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCt                                                                                                 >  4:12610/1‑149 (MQ=255)
                                                gggCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGcc                                                                                             >  2:128428/1‑149 (MQ=255)
                                                 ggCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCct                                                                                            >  5:3394/1‑149 (MQ=255)
                                                     gtggtgCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTAATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGActtctt                                                                                        >  1:264356/1‑149 (MQ=255)
                                                      tggtgCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCttcttc                                                                                       <  3:51509/149‑1 (MQ=255)
                                                      tggtgCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCttcttc                                                                                       <  1:146146/149‑1 (MQ=255)
                                                        gtgCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCAc                                                                                     <  4:230198/149‑1 (MQ=255)
                                                           cTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTc                                                                                                      >  1:120653/1‑129 (MQ=255)
                                                                    ggcggcACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCATTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTgg                                                                          <  3:44979/148‑1 (MQ=255)
                                                                          aCGGCTTTGCTCGGCACCCTCCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCt                                                                   >  2:220194/1‑149 (MQ=255)
                                                                            ggCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTg                                                                 >  5:15490/1‑149 (MQ=255)
                                                                            ggCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTg                                                                 <  1:128428/149‑1 (MQ=255)
                                                                            ggCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTg                                                                 <  3:12610/149‑1 (MQ=255)
                                                                             gCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAg                                                                                 >  1:138917/1‑132 (MQ=255)
                                                                             gCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAg                                                                                 <  2:138917/132‑1 (MQ=255)
                                                                                    cccggaCCATGCCACAGACGCTCAAGGTCGTAGAACTGGCGCGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGAc                                                         <  5:264983/144‑1 (MQ=255)
                                                                                      ggCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCACTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTg                                                       >  4:170104/1‑149 (MQ=255)
                                                                                           ccTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCa                                                  <  8:43625/149‑1 (MQ=255)
                                                                                                cacaGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGAc                                             <  6:15490/149‑1 (MQ=255)
                                                                                                 acaGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCt                                                                            >  5:304006/1‑117 (MQ=255)
                                                                                                 acaGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCt                                                                            <  6:304006/117‑1 (MQ=255)
                                                                                                     aCGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGc                                                                                              <  1:136931/95‑1 (MQ=255)
                                                                                                     aCGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGc                                                                                              >  2:136931/1‑95 (MQ=255)
                                                                                                       gCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCt                                                     <  2:31428/134‑1 (MQ=255)
                                                                                                       gCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCt                                                     >  1:31428/1‑134 (MQ=255)
                                                                                                         tCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGc                                    >  5:285116/1‑149 (MQ=255)
                                                                                                              gTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATcg                               >  3:298815/1‑149 (MQ=255)
                                                                                                                 gTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGt                            >  1:66839/1‑149 (MQ=255)
                                                                                                                          gCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGc                   <  3:170104/149‑1 (MQ=255)
                                                                                                                            gggCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCgg                 >  8:124218/1‑149 (MQ=255)
                                                                                                                               cAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTg              <  6:3394/149‑1 (MQ=255)
                                                                                                                                  acgacgTCATCGTGCACATGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCg                  >  5:88028/6‑142 (MQ=255)
                                                                                                                                  acgacgTCATCGTGCACATGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCg                  <  6:88028/137‑1 (MQ=255)
                                                                                                                                     cggTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTa        >  7:520/1‑149 (MQ=255)
                                                                                                                                       gTCATCGTGCACATGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTTGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAg           <  6:145316/144‑1 (MQ=255)
                                                                                                                                       gTCATCGTGCACATGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAg           >  5:145316/1‑144 (MQ=255)
                                                                                                                                       gTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGGTGGAGGTAc       <  1:307664/148‑1 (MQ=255)
                                                                                                                                       gTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGATGGAGGTAc       >  2:307664/1‑148 (MQ=255)
                                                                                                                                          atcatGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCTCCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTACCGGt   <  2:264356/149‑1 (MQ=255)
                                                                                                                                          atcatGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTACCGGt   >  6:149320/1‑149 (MQ=255)
                                                                                                                                           tcatGAGCACGATGACGTCTTGCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTACCGGTg  <  3:204973/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
CCTTATTCGCGGTCTTTGAGCTTGTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTACCGGTG  >  NC_002947/5472397‑5472684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑