Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,472,545 C→T 7.2% V92M (GTG→ATG)  rsfS ← ribosomal silencing factor RsfS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,472,5450CT 7.2% 156.7 / 2.3 55V92M (GTG→ATG) rsfSribosomal silencing factor RsfS
Reads supporting (aligned to +/- strand):  ref base C (24/27);  new base T (2/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

ATTCGCGGTCTTTGAGCTTGTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTACCGGTGGCAATGA  >  NC_002947/5472401‑5472691
                                                                                                                                                |                                                                                                                                                  
aTTCGCGGTCTTTGAGCTTGTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGAt                                                                                                                                                 <  2:23334/148‑1 (MQ=255)
          tttGAGCTTGTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGtt                                                                                                                                        >  1:286960/1‑147 (MQ=255)
            tGAGCTTGTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAg                                                                                                                                     <  2:102772/148‑1 (MQ=255)
                  tGTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGTCATCGGCGGAACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGAAGCGGCGGTCATCATGTGCACGATGACGTCGTAGAGGTCcag                                                                                                                               >  4:44979/1‑148 (MQ=255)
                   gTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCcagc                                                                                                                              <  8:212650/148‑1 (MQ=255)
                   gTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCcag                                                                                                                               <  3:129302/147‑1 (MQ=255)
                   gTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCcag                                                                                                                               <  6:33321/147‑1 (MQ=255)
                             tCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTc                                                                                                                   >  3:230198/1‑149 (MQ=255)
                                     aTGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGc                                                                                                           >  7:43625/1‑149 (MQ=255)
                                      tGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGcc                                                                                                          <  2:142825/149‑1 (MQ=255)
                                        gTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCt                                                                                                        >  4:12610/1‑149 (MQ=255)
                                            gggCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGcc                                                                                                    >  2:128428/1‑149 (MQ=255)
                                             ggCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCct                                                                                                   >  5:3394/1‑149 (MQ=255)
                                                 gtggtgCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTAATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGActtctt                                                                                               >  1:264356/1‑149 (MQ=255)
                                                  tggtgCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCttcttc                                                                                              <  3:51509/149‑1 (MQ=255)
                                                  tggtgCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCttcttc                                                                                              <  1:146146/149‑1 (MQ=255)
                                                    gtgCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCAc                                                                                            <  4:230198/149‑1 (MQ=255)
                                                       cTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTc                                                                                                             >  1:120653/1‑129 (MQ=255)
                                                                ggcggcACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCATTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTgg                                                                                 <  3:44979/148‑1 (MQ=255)
                                                                      aCGGCTTTGCTCGGCACCCTCCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCt                                                                          >  2:220194/1‑149 (MQ=255)
                                                                        ggCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTg                                                                        <  1:128428/149‑1 (MQ=255)
                                                                        ggCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTg                                                                        >  5:15490/1‑149 (MQ=255)
                                                                        ggCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTg                                                                        <  3:12610/149‑1 (MQ=255)
                                                                         gCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAg                                                                                        >  1:138917/1‑132 (MQ=255)
                                                                         gCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAg                                                                                        <  2:138917/132‑1 (MQ=255)
                                                                                cccggaCCATGCCACAGACGCTCAAGGTCGTAGAACTGGCGCGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGAc                                                                <  5:264983/144‑1 (MQ=255)
                                                                                  ggCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCACTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTg                                                              >  4:170104/1‑149 (MQ=255)
                                                                                       ccTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCa                                                         <  8:43625/149‑1 (MQ=255)
                                                                                            cacaGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGAc                                                    <  6:15490/149‑1 (MQ=255)
                                                                                             acaGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCt                                                                                   <  6:304006/117‑1 (MQ=255)
                                                                                             acaGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCt                                                                                   >  5:304006/1‑117 (MQ=255)
                                                                                                 aCGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGc                                                                                                     <  1:136931/95‑1 (MQ=255)
                                                                                                 aCGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGc                                                                                                     >  2:136931/1‑95 (MQ=255)
                                                                                                   gCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCt                                                            <  2:31428/134‑1 (MQ=255)
                                                                                                   gCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCt                                                            >  1:31428/1‑134 (MQ=255)
                                                                                                     tCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGc                                           >  5:285116/1‑149 (MQ=255)
                                                                                                          gTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATcg                                      >  3:298815/1‑149 (MQ=255)
                                                                                                             gTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGt                                   >  1:66839/1‑149 (MQ=255)
                                                                                                                      gCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGc                          <  3:170104/149‑1 (MQ=255)
                                                                                                                        gggCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCgg                        >  8:124218/1‑149 (MQ=255)
                                                                                                                           cAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTg                     <  6:3394/149‑1 (MQ=255)
                                                                                                                              acgacgTCATCGTGCACATGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCg                         <  6:88028/137‑1 (MQ=255)
                                                                                                                              acgacgTCATCGTGCACATGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCg                         >  5:88028/6‑142 (MQ=255)
                                                                                                                                 cggTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTa               >  7:520/1‑149 (MQ=255)
                                                                                                                                   gTCATCGTGCACATGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTTGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAg                  <  6:145316/144‑1 (MQ=255)
                                                                                                                                   gTCATCGTGCACATGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAg                  >  5:145316/1‑144 (MQ=255)
                                                                                                                                   gTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGGTGGAGGTAc              <  1:307664/148‑1 (MQ=255)
                                                                                                                                   gTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGATGGAGGTAc              >  2:307664/1‑148 (MQ=255)
                                                                                                                                      atcatGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCTCCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTACCGGt          <  2:264356/149‑1 (MQ=255)
                                                                                                                                      atcatGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTACCGGt          >  6:149320/1‑149 (MQ=255)
                                                                                                                                       tcatGAGCACGATGACGTCTTGCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTACCGGTg         <  3:204973/149‑1 (MQ=255)
                                                                                                                                            tgCACGCTGACGTCGTTCATGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTACCGGTGGCaa     <  2:286960/148‑1 (MQ=255)
                                                                                                                                             gCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTACCgg           <  3:205197/141‑1 (MQ=255)
                                                                                                                                             gCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTACCgg           >  4:205197/1‑141 (MQ=255)
                                                                                                                                              cACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTACCGGTGGCAATGa  <  2:66839/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
ATTCGCGGTCTTTGAGCTTGTCGGAGTACTCGTGGGCATGGTCCGGGCTGTGGTGCTTGCCATCGGCGGCACGGCTTTGCTCGGCACCCTGCCACAGACGCTCAAGGTCGTAGAACTGGCGGGCAGCGGCGGTCATCATGTGCACGATGACGTCGTTCAGGTCCAGCAGCACCCAGTCGCTGTCGCCCTTGCCTTCTTCACCCAGTGGCTGGGCGCCCTTGGCCTTGACTGCTTCACGGACCTTTTCGGCCATCGCGTTGATCTGGCGGTTGGAGGTACCGGTGGCAATGA  >  NC_002947/5472401‑5472691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑