Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,485,154 T→C 20.7% intergenic (+60/‑87) purH → / → purD bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,485,1540TC20.7% 58.4 / 11.8 36intergenic (+60/‑87)purH/purDbifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base T (12/15);  new base C (4/3);  total (17/19)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.08e-01

TTCGATGCGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCA  >  NC_002947/5485007‑5485302
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ttCGATGCGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTTCCCGCGCATACGCtg                                                                                                                                                     >  8:160363/1‑149 (MQ=255)
ttCGATGCGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGAtg                                                                                                                                                     >  3:295392/1‑149 (MQ=255)
     tGCGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGa                                                                                                                                                <  7:95351/149‑1 (MQ=255)
        gTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGAtggt                                                                                                                                                   >  6:160985/1‑143 (MQ=255)
        gTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGAtggt                                                                                                                                                   <  5:160985/143‑1 (MQ=255)
           aTGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCAcc                                                                                                                                          <  3:68622/149‑1 (MQ=255)
               tGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTTCCCGCGCATAAGATGGTGGATTCAccgccc                                                                                                                                      >  2:50684/1‑148 (MQ=255)
                gAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGGCCGCGAATAACATGGTGGATTAAcccacc                                                                                                                                      >  6:224257/1‑144 (MQ=255)
                  aGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGGCGGTGAATCCAccatcgtat                                                                                                                                   >  6:112470/1‑142 (MQ=38)
                  aGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCAt                                                                                                                                   <  6:229827/149‑1 (MQ=255)
                      aTCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGGCGGTGAATCCACCATCGTATTcgc                                                                                                                               <  5:112470/149‑1 (MQ=37)
                      aTCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCa                                                                                                                                    <  4:199576/144‑1 (MQ=255)
                      aTCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCa                                                                                                                                    >  3:199576/1‑144 (MQ=255)
                          ctgctgCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCggg                                                                                                                            >  5:304417/1‑148 (MQ=255)
                               gcCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTTCCCCCGAAAAAGAAGGTGGATTAACCGCCGCAATAGCGGGCAAGc                                                                                                                       >  4:297647/1‑148 (MQ=255)
                               gcCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGc                                                                                                                       <  3:297647/148‑1 (MQ=255)
                                ccGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGCATAAGATGGGGGGTTGACCGCCGCCATAGCGGGCCAGccc                                                                                                                     >  3:38986/1‑149 (MQ=255)
                                ccGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGc                                                                                                                       <  4:295392/147‑1 (MQ=255)
                                ccGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGGGGATTCACCGCCGCATTCGCGGGCAAGc                                                                                                                       <  3:61924/147‑1 (MQ=255)
                                     gaGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGGCCGCGCATAAGATTGTGGGTTTCCCCCCGGCTTAGCGGGGCAGGCCGCTcg                                                                                                                >  3:101972/1‑148 (MQ=255)
                                              agcgcgATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTCCGCGGATCCCGAGCCAAGTCCAGATCCTGGGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGtt                                                                                                       <  7:160363/147‑1 (MQ=255)
                                                       gTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGATATCCTGTGGGAGCGGGCTTGCCCGCGAATACTATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCaa                                                                                               <  1:94164/148‑1 (MQ=255)
                                                            cACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGCCAAGGCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGgc                                                                                                   <  5:73315/139‑1 (MQ=255)
                                                                 gCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATc                                                                                    <  6:304417/149‑1 (MQ=255)
                                                                  cATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGGCGGTGAATCCAccat                                                                                                                                        <  4:174026/96‑3 (MQ=25)
                                                                  cATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGGCGGTGAATCCAccat                                                                                                                                        >  3:174026/1‑94 (MQ=25)
                                                                   aTGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGTCCGCGAATGCGGCGGTGAATCCACCATCGTATTcg                                                                                                                                <  4:127792/103‑1 (MQ=18)
                                                                   aTGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGTCCGCGAATGCGGCGGTGAATCCACCATCGTATTcg                                                                                                                                >  3:127792/1‑103 (MQ=18)
                                                                              ccGCCAATAATTCCGCGTATCCCGAGGCATGGCGAGATCCTGTGGGAGCGGGCTTGCCCGCTAATACGTGGGTGTATTCCCCGCCGCTTTCGGGGGAAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAg                                                                        <  4:38986/148‑1 (MQ=37)
                                                                                           cgcgGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCGGCTAATACGTTGGTGGATTCACCGCCGCATTCGGGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGaaa                                                          <  5:224257/149‑1 (MQ=255)
                                                                                                                        tgGGAGCGGGCTTGCCCGCGTATAGGTTGGTGGATTCACGGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGGTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGa                             <  1:50684/149‑1 (MQ=39)
                                                                                                                              cGGGCTTGCCCGCTAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGc                       <  3:248864/149‑1 (MQ=255)
                                                                                                                               gggCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGcc                      >  4:143441/1‑149 (MQ=255)
                                                                                                                                ggCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGcac                     >  6:250845/1‑147 (MQ=255)
                                                                                                                                 gCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcct                    >  6:313128/1‑149 (MQ=255)
                                                                                                                                        cgcgAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctg              >  2:159915/1‑148 (MQ=255)
                                                                                                                                         gcgAATGCGGCGGTGAATCCACCATCGTATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATTAAATTTTTGATAATCTGCAGCGGTGGCCGTGAGCACGCcctggcctgg             >  8:252233/1‑148 (MQ=25)
                                                                                                                                            aaTACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAg         >  7:182315/1‑149 (MQ=255)
                                                                                                                                              tACGATGGTGGATTCACCGCCGCATTCGCGGGAAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGTATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTc       <  7:199899/149‑1 (MQ=255)
                                                                                                                                                 gATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGcc    >  6:289073/1‑149 (MQ=255)
                                                                                                                                                   tggtggATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCa  <  1:93307/149‑1 (MQ=255)
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TTCGATGCGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCA  >  NC_002947/5485007‑5485302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑