| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,485,162 | T→C | 20.5% | intergenic (+68/‑79) | purH → / → purD | bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,485,162 | 0 | T | C | 20.5% | 69.5 / 14.4 | 39 | intergenic (+68/‑79) | purH/purD | bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase |
| Reads supporting (aligned to +/- strand): ref base T (14/17); new base C (4/4); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.32e-01 | |||||||||||
ATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCA‑CGCCCTGGCCTGGAAAGTCGCCCAGGA > NC_002947/5485018‑5485305 | aTGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCAcc < 3:68622/149‑1 (MQ=255) tGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTTCCCGCGCATAAGATGGTGGATTCAccgccc > 2:50684/1‑148 (MQ=255) gAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGGCCGCGAATAACATGGTGGATTAAcccacc > 6:224257/1‑144 (MQ=255) aGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGGCGGTGAATCCAccatcgtat > 6:112470/1‑142 (MQ=38) aGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCAt < 6:229827/149‑1 (MQ=255) aTCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGGCGGTGAATCCACCATCGTATTcgc < 5:112470/149‑1 (MQ=37) aTCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCa < 4:199576/144‑1 (MQ=255) aTCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCa > 3:199576/1‑144 (MQ=255) ctgctgCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCggg > 5:304417/1‑148 (MQ=255) gcCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTTCCCCCGAAAAAGAAGGTGGATTAACCGCCGCAATAGCGGGCAAGc > 4:297647/1‑148 (MQ=255) gcCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGc < 3:297647/148‑1 (MQ=255) ccGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGCATAAGATGGGGGGTTGACCGCCGCCATAGCGGGCCAGccc > 3:38986/1‑149 (MQ=255) ccGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGc < 4:295392/147‑1 (MQ=255) ccGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGGGGATTCACCGCCGCATTCGCGGGCAAGc < 3:61924/147‑1 (MQ=255) gaGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGGCCGCGCATAAGATTGTGGGTTTCCCCCCGGCTTAGCGGGGCAGGCCGCTcg > 3:101972/1‑148 (MQ=255) agcgcgATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTCCGCGGATCCCGAGCCAAGTCCAGATCCTGGGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGtt < 7:160363/147‑1 (MQ=255) gTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGATATCCTGTGGGAGCGGGCTTGCCCGCGAATACTATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCaa < 1:94164/148‑1 (MQ=255) cACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGCCAAGGCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGgc < 5:73315/139‑1 (MQ=255) gCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATc < 6:304417/149‑1 (MQ=255) cATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGGCGGTGAATCCAccat > 3:174026/1‑94 (MQ=25) cATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGGCGGTGAATCCAccat < 4:174026/96‑3 (MQ=25) aTGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGTCCGCGAATGCGGCGGTGAATCCACCATCGTATTcg < 4:127792/103‑1 (MQ=18) aTGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGTCCGCGAATGCGGCGGTGAATCCACCATCGTATTcg > 3:127792/1‑103 (MQ=18) ccGCCAATAATTCCGCGTATCCCGAGGCATGGCGAGATCCTGTGGGAGCGGGCTTGCCCGCTAATACGTGGGTGTATTCCCCGCCGCTTTCGGGGGAAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAg < 4:38986/148‑1 (MQ=37) cgcgGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCGGCTAATACGTTGGTGGATTCACCGCCGCATTCGGGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGaaa < 5:224257/149‑1 (MQ=255) tgGGAGCGGGCTTGCCCGCGTATAGGTTGGTGGATTCACGGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGGTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGa < 1:50684/149‑1 (MQ=39) cGGGCTTGCCCGCTAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCA‑CGc < 3:248864/149‑1 (MQ=255) gggCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCA‑CGcc > 4:143441/1‑149 (MQ=255) ggCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCA‑CGcac > 6:250845/1‑147 (MQ=255) gCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCA‑CGCcct > 6:313128/1‑149 (MQ=255) cgcgAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCA‑CGCcctggcctg > 2:159915/1‑148 (MQ=255) gcgAATGCGGCGGTGAATCCACCATCGTATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATTAAATTTTTGATAATCTGCAGCGGTGGCCGTGAGCA‑CGCcctggcctgg > 8:252233/1‑148 (MQ=25) aaTACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCA‑CGCCCTGGCCTGGAAAg > 7:182315/1‑149 (MQ=255) tACGATGGTGGATTCACCGCCGCATTCGCGGGAAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGTATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCA‑CGCCCTGGCCTGGAAAGTc < 7:199899/149‑1 (MQ=255) gATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCA‑CGCCCTGGCCTGGAAAGTCGcc > 6:289073/1‑149 (MQ=255) tggtggATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCA‑CGCCCTGGCCTGGAAAGTCGCCCa < 1:93307/149‑1 (MQ=255) cggtgaATCCACCATCGTATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCA‑CGCCCTGGCCTGGAAAGTCGCCCa < 7:252233/148‑1 (MQ=38) gtggATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCA‑CGCCCTGGCCTGGAAAGTCGCCCAg > 5:289266/1‑148 (MQ=255) gATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACCGCCCTGGCCTGGAAAGTCGCCCAGGa < 1:159915/148‑1 (MQ=255) | ATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCA‑CGCCCTGGCCTGGAAAGTCGCCCAGGA > NC_002947/5485018‑5485305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |