Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,487,904 C→G 8.1% T411T (ACC→ACG)  PP_4824 → two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,487,9040CG 8.1% 211.3 / 9.6 74T411T (ACC→ACG) PP_4824two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base C (30/38);  new base G (3/3);  total (33/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-02

TGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACGAAATTCTGGACATTTCCAAGCTG  >  NC_002947/5487757‑5488048
                                                                                                                                                      |                                                                                                                                                 
tGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCAccc                                                                                                                                                  >  3:130400/1‑149 (MQ=255)
 ggCATGATCCTCAACCTGGCGGTAAGTGATCGGTGGCTGGGCATATGCGTGGAGCGTTTCTGCGCCTGCAGTGCAGTTTCCTCCAGC‑‑‑GATGCGGAAATCAACTCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCAccc                                                                                                                                                  <  6:199841/148‑1 (MQ=255)
  gCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCAcccc                                                                                                                                                 >  2:79490/1‑148 (MQ=255)
   ccTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGa                                                                                                                                               <  3:263584/149‑1 (MQ=255)
    cTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGAt                                                                                                                                              <  8:160518/149‑1 (MQ=255)
          ctcAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACgg                                                                                                                                        >  5:33439/1‑149 (MQ=255)
          ctcAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACgg                                                                                                                                        >  3:100031/1‑149 (MQ=255)
             aaCCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGc                                                                                                                                       <  1:161165/147‑1 (MQ=255)
             aaCCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCAACGAAATCCGCACCCCGATGAACGGCGt                                                                                                                                     >  8:79443/1‑149 (MQ=255)
                cTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCAACGAAATCCGCACCCCGATGAACGGCGTGCt                                                                                                                                  >  8:262428/1‑149 (MQ=255)
                 tGGCGGTCAGTGAACGGCTGCGTCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTc                                                                                                                                 >  8:55841/1‑149 (MQ=255)
                   gCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTc                                                                                                                                 <  6:33439/147‑1 (MQ=255)
                   gCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCgg                                                                                                                               <  5:254515/149‑1 (MQ=255)
                    cGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGc                                                                                                                              <  3:21967/149‑1 (MQ=255)
                     ggTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACGCCGTTCATCGGGGTGcggattt                                                                                                                             <  3:258939/149‑7 (MQ=255)
                      gTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCTGCCGTGCACTGGCCGCCAGCGTGGAGGCGAAAAACACCCCAAAAGCCGATTTTATGGCAAAAAAAACCCACAAAAACCGCCCCCCGATGAACGGCGTGCTCgg                                                                                                                               >  1:95341/1‑149 (MQ=255)
                         aGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGAc                                                                                                                         >  2:290853/1‑149 (MQ=255)
                           tGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACGCCGTTCATCGGGGTGcgga                                                                                                                                >  6:84251/1‑137 (MQ=255)
                           tGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACGCCGTTCATCGGGGTGcgga                                                                                                                                <  5:84251/140‑4 (MQ=255)
                           tGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACt                                                                                                                        >  3:295957/1‑148 (MQ=255)
                            gAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTg                                                                                                                       <  7:262428/148‑1 (MQ=255)
                              aCGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGaa                                                                                                                     <  2:263251/148‑1 (MQ=255)
                              aCGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAAc                                                                                                                    <  5:270817/149‑1 (MQ=255)
                               cGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAAct                                                                                                                   <  7:55841/149‑1 (MQ=255)
                                  cTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAActgct                                                                                                                >  4:214961/1‑149 (MQ=255)
                                     cggcgCATAAGCGAGGAGTGTTTCAGCTCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCCGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAActgctgct                                                                                                             <  8:19782/149‑1 (MQ=255)
                                       gcgcATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTgg                                                                                                           <  4:100031/149‑1 (MQ=255)
                                             aaGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACGCCGTTCATCGGGGTGcggat                                                                                                                               <  7:250946/123‑5 (MQ=255)
                                             aaGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACGCCGTTCATCGGGGTGcggat                                                                                                                               >  8:250946/1‑119 (MQ=255)
                                               gCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCg                                                                                                   >  3:57249/1‑149 (MQ=255)
                                                 gaggagCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCt                                                                                                 >  8:223778/1‑149 (MQ=255)
                                                  aggagCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTgg                                                                                                           <  4:264313/138‑1 (MQ=255)
                                                  aggagCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTgg                                                                                                           >  3:264313/1‑138 (MQ=255)
                                                   ggagCGTTTCAGCGCCAGCCGTGCACTGGCCTCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑t                                                                                              <  1:142980/149‑1 (MQ=255)
                                                      gCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCAGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCgg                                                                                           <  1:79490/149‑1 (MQ=255)
                                                       cGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCGTAGATCAGCCACGAAATGCGCAGCCCGATGAACGGGGTGCTCGGCATGACTGAACTGCTGCTGGGCACTCGGCTG‑TCgg                                                                                           <  4:215348/148‑1 (MQ=255)
                                                         gttCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTg                                                                                         >  3:150085/2‑148 (MQ=255)
                                                           tCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGaa                                                                                       <  7:223778/148‑1 (MQ=255)
                                                           tCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGaa                                                                                       <  4:130400/148‑1 (MQ=255)
                                                                       gTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTAt                                                                          >  2:42117/1‑149 (MQ=255)
                                                                       gTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTAt                                                                          <  4:295957/149‑1 (MQ=255)
                                                                               gccgccAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGc                                                                                                         <  2:233619/111‑1 (MQ=255)
                                                                               gccgccAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGc                                                                                                         >  1:233619/1‑111 (MQ=255)
                                                                               gccgccAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGAc                                                                  >  3:301800/1‑149 (MQ=255)
                                                                                  gccAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCAt                                                               <  3:167166/149‑1 (MQ=255)
                                                                                     aGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCca                                                            <  3:214961/149‑1 (MQ=255)
                                                                                           gATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGAtt                                                                            <  6:169324/130‑1 (MQ=255)
                                                                                           gATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGAtt                                                                            >  5:169324/1‑130 (MQ=255)
                                                                                               cGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGcgcg                                                     >  5:173311/1‑149 (MQ=255)
                                                                                                ggAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCgg                                                    <  3:132962/149‑1 (MQ=255)
                                                                                                ggAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCgg                                                    <  1:290853/149‑1 (MQ=255)
                                                                                                     tCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAAc                                               <  4:150085/149‑1 (MQ=255)
                                                                                                       aaCGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAAc                                               >  8:97828/1‑147 (MQ=255)
                                                                                                                   gAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCt                                 >  1:36481/1‑149 (MQ=255)
                                                                                                                      tttCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGa                               >  7:262236/1‑148 (MQ=255)
                                                                                                                       ttCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGGCTGAACTGCTGCTGGGCACACCGCTG‑TCGGTg                                                                                         >  1:238610/1‑89 (MQ=255)
                                                                                                                       ttCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTg                                                                                         <  2:238610/89‑1 (MQ=255)
                                                                                                                            gCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACg                         >  8:173059/1‑148 (MQ=255)
                                                                                                                            gCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACg                         >  3:27856/1‑148 (MQ=255)
                                                                                                                                   tCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACGAAATTCTg                 <  6:173311/149‑1 (MQ=255)
                                                                                                                                    cAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGAtt                                                                            >  4:150912/1‑89 (MQ=255)
                                                                                                                                    cAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGAtt                                                                            <  3:150912/89‑1 (MQ=255)
                                                                                                                                     aGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACGAAATTCTGGa               <  2:95341/149‑1 (MQ=255)
                                                                                                                                       ccACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACGAAATTCTGGACa             >  4:67743/1‑149 (MQ=255)
                                                                                                                                        cACGAAATCAGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACGAAATTCTGGACAt            >  5:137120/1‑149 (MQ=255)
                                                                                                                                          cGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACGAAATTCTGGACAttt          >  8:201864/1‑149 (MQ=255)
                                                                                                                                           gAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACGAAATTCTGGACATTTc         >  1:154607/1‑149 (MQ=255)
                                                                                                                                             aaTCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACGAAATTCTGGACATTTCCa       <  3:67743/149‑1 (MQ=255)
                                                                                                                                                gagCACGCCGTTCATCGGGGTGCTCGGCATGACTGAACTGCTGCTGCGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGCGCTGCTGACGCTGATCAACGAAATTCTGTACATTTCCAAGc    >  7:21218/3‑149 (MQ=255)
                                                                                                                                                 cGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACGATATTCTGGACATTTCCAAGc    <  4:301800/148‑1 (MQ=255)
                                                                                                                                                 cGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACGAAATTCTGGACATTTCCAAGCt   <  2:154607/149‑1 (MQ=255)
                                                                                                                                                  gCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACTAGCTGCTGACGCTGATCAACGAAATTCTGGACATTTCCAAGCTg  <  7:79443/149‑1 (MQ=255)
                                                                                                                                                  gCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACg                         <  5:134562/126‑1 (MQ=255)
                                                                                                                                                  gCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACg                         >  6:134562/1‑126 (MQ=255)
                                                                                                                                                  gCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACGAAATTCTGGACATTTCCAAGCTg  <  7:97828/149‑1 (MQ=255)
                                                                                                                                                  gCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACGAAATTCTGGACATTTCCAAGCTg  <  2:36481/149‑1 (MQ=255)
                                                                                                                                                    aCCCCGATTAACGTTGTGCTCTTCATTACTTAACTGCTGGTTTTAACACAGGTGTTCGGT‑AAGAAACGTGATTCTGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACGAAATTCTGGACATTTCCAAGCTg  <  1:250383/147‑1 (MQ=255)
                                                                                                                                                      cccGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCgg                                                    >  2:166823/1‑95 (MQ=255)
                                                                                                                                                      cccGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCgg                                                    <  1:166823/95‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
TGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGC‑‑‑GATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAATCCGCACCCCGATGAACGGCGTGCTCGGCATGACTGAACTGCTGCTGGGCACACCGCTG‑TCGGTGAAGCAACGTGATTATGTGCAGACCATCCACAGCGCGGGCAACGAGCTGCTGACGCTGATCAACGAAATTCTGGACATTTCCAAGCTG  >  NC_002947/5487757‑5488048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑