Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,611,703	G→A	7.1%	A233T (GCC→ACC)	PP_4931 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,611,703	0	G	A	7.1%	205.1 / 4.3	70	A233T (GCC→ACC)	PP_4931	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (30/35);  new base A (3/2);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01

ATGCGTTGGTCGGCCTGCGCGTGGACGACCGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTACCTCGGTGGGGACCTGGCCGAAGCCGATGGCGTGGC  >  NC_002947/5611557‑5611848                                                                                                                                                   |                                                                                                                                                   aTGCGTTGGTCGGCCTGCGCGTGGACGACCGGGAAATCCGTGCCCAACGTATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGc                                                                                                                                                   >  4:300911/1‑148 (MQ=255) aTGCGTTGGTCGGCCTGCGCGTGGACGACCGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATACAAACCCGCCCGCTGCACATGGTCATGGc                                                                                                                                                   <  8:131927/148‑1 (MQ=255)     gTTGGTCGGCCTGCGCGTGGACGACCGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCGGCCCGCTGCACATGGTCATGGCCAAgg                                                                                                                                              <  5:83812/149‑1 (MQ=255)     gTTGGTCGGCCTGCGCGTGGACGACCGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGACCAAgg                                                                                                                                              >  8:249334/1‑149 (MQ=255)     gTTGGTCGGCCTGCGCGTGGACGACCGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGACCAAgg                                                                                                                                              >  2:963/1‑149 (MQ=255)     gTTGGTCGGCCTGCGCGTGGACGACCGGGAAATCCGTGCCCAACGAATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACACGCCCGCTGCACATGGTCATGGCCAAgg                                                                                                                                              >  4:176523/1‑149 (MQ=255)        ggTCGGCCTGCGCGTGGACGACCGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAACCCCGCCCGCTGCACATGGTCATGGCCAAGGGcc                                                                                                                                           >  6:277249/1‑149 (MQ=255)        ggTCGGCCTGCGCGTGGACGACCGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGc                                                                                                                                            <  2:252816/148‑1 (MQ=255)          tCGGCCTGCGCGTGGACGACCGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGTCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCg                                                                                                                                         <  2:285949/149‑1 (MQ=255)            ggCCTGCGCGTGGACGACCGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGaa                                                                                                                                       <  6:204498/149‑1 (MQ=255)             gCCTGCGCGTGGACGACCGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAAc                                                                                                                                      <  4:201163/149‑1 (MQ=255)                        gacgacCGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCtt                                                                                                                            <  5:310457/148‑1 (MQ=255)                        gacgacCGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGACCAAGGGCCCGAACCTCAAACCttt                                                                                                                           <  1:963/149‑1 (MQ=255)                        gacgacCGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGACCAAGGGCCCGAACCTCAAACCttt                                                                                                                           <  7:249334/149‑1 (MQ=255)                             ccGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACg                                                                                                                      >  6:281140/1‑149 (MQ=255)                              cGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGc                                                                                                                     >  6:172796/1‑149 (MQ=255)                              cGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAAGGCCCGAACCTCAAACCTTTGTACGc                                                                                                                     >  4:113319/1‑149 (MQ=255)                               gggAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGcc                                                                                                                    <  3:110521/149‑1 (MQ=255)                                   aaTCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACt                                                                                                                <  7:7148/149‑1 (MQ=255)                                           cccAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTcggc                                                                                                        >  5:129955/1‑149 (MQ=255)                                            ccAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTcggcg                                                                                                       >  2:124507/1‑149 (MQ=255)                                             cAACGCATCGTATTGAGCGCCGGAGCCGGAACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAg                                                                                                                                               >  3:169996/1‑108 (MQ=255)                                             cAACGCATCGTATTGAGCGCCGGAGCCGGAACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAg                                                                                                                                               <  4:169996/108‑1 (MQ=255)                                               aCGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTcggcggcg                                                                                                    <  3:67109/149‑1 (MQ=255)                                               aCGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTcggcggcg                                                                                                    >  2:201614/1‑149 (MQ=255)                                                  cATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGc                                                                                                 <  5:281140/149‑1 (MQ=255)                                                   aTCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGcc                                                                                                <  4:255906/149‑1 (MQ=255)                                                            aGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCcgcg                                                                                       >  3:118856/1‑149 (MQ=255)                                                                ccGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCcgcg                                                                                       >  2:299260/1‑145 (MQ=255)                                                                ccGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCcgcg                                                                                       <  1:299260/145‑1 (MQ=255)                                                                        ggCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCa                                                                                                                                  >  4:279391/1‑94 (MQ=255)                                                                        ggCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCa                                                                                                                                  <  3:279391/94‑1 (MQ=255)                                                                        ggCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGac                                                                          <  4:274211/149‑1 (MQ=255)                                                                          cACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGacca                                                                        <  5:277249/149‑1 (MQ=255)                                                                          cACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGacca                                                                        <  5:172796/149‑1 (MQ=255)                                                                           aCCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGaccac                                                                       <  2:206949/149‑1 (MQ=255)                                                                           aCCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGaccac                                                                       >  2:224223/1‑149 (MQ=255)                                                                           aCCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGaccac                                                                       <  3:300911/149‑1 (MQ=255)                                                                            ccGTAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGaccacc                                                                      >  4:79807/1‑149 (MQ=255)                                                                             cGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGaccacc                                                                      >  2:43276/1‑148 (MQ=255)                                                                              gAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGACCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCa                                                                    >  2:195998/1‑149 (MQ=255)                                                                              gAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCCGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCa                                                                    >  1:202186/1‑149 (MQ=255)                                                                                    ttGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCgg                                                               >  2:203691/1‑148 (MQ=255)                                                                                           aGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGCCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGAt                                                       <  3:176523/149‑1 (MQ=255)                                                                                             gcgcTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATgg                                                     <  1:43276/149‑1 (MQ=255)                                                                                                   ggCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGACCCTCACACCTTTCTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCCCGCGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTg                                               >  8:159585/1‑149 (MQ=255)                                                                                                   ggCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGTCAACGGTGACCACCCATCCGGCAGCCGATGGCCAGt                                                <  1:201614/149‑1 (MQ=255)                                                                                                    gCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAAGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTgg                                              <  3:113319/149‑1 (MQ=255)                                                                                                     ccTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTggg                                             >  5:131054/1‑149 (MQ=255)                                                                                                     ccTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTggg                                             >  2:60195/1‑149 (MQ=255)                                                                                                      cTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGt                                            >  6:25355/1‑149 (MQ=255)                                                                                                         gATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAATCCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTg                                         <  1:203691/149‑1 (MQ=255)                                                                                                         gATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTc                                           <  1:60195/147‑1 (MQ=255)                                                                                                          aTCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACg                                                                               <  5:220095/110‑1 (MQ=255)                                                                                                          aTCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACg                                                                               >  6:220095/1‑110 (MQ=255)                                                                                                           tCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGt                                       <  4:118856/149‑1 (MQ=255)                                                                                                           tCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGt                                       <  1:224223/149‑1 (MQ=255)                                                                                                             aGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTAc                                     >  3:54187/1‑149 (MQ=255)                                                                                                               ccGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTACCt                                   <  5:25355/149‑1 (MQ=255)                                                                                                                cGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTACCTc                                  >  3:231107/1‑149 (MQ=255)                                                                                                                cGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTACCTc                                  >  3:235263/1‑149 (MQ=255)                                                                                                                   ccATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTACCTCGGt                               <  3:79807/149‑1 (MQ=255)                                                                                                                       gCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTACCTCGGTggg                            <  1:176862/148‑1 (MQ=255)                                                                                                                       gCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTACCTCGGTggg                            >  2:176862/1‑148 (MQ=255)                                                                                                                            cccgcccgCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTACCTCGGTGGGGACCTg                      >  5:159478/1‑149 (MQ=255)                                                                                                                                   gcTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTACCTCGGTGGGGACCTGGCCGAAg               <  4:54187/149‑1 (MQ=255)                                                                                                                                             gTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTACCTCGGTGGGGACCTGGCCGAAGCCGAtggcg      <  1:124507/148‑1 (MQ=255)                                                                                                                                              tCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTACCTCGGTGGGGACCTGGCCGAAGCCGAtggcgtg    >  7:214112/1‑149 (MQ=255)                                                                                                                                                aTGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTACCTCGGTGGGGACCTGGCCGAAGCCGAtggcgtggc  >  6:236664/1‑149 (MQ=255)                                                                                                                                                aTGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTACCTCGGTGGGGACCTGGCCGAAGCCGAtggcgtggc  <  6:129955/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   ATGCGTTGGTCGGCCTGCGCGTGGACGACCGGGAAATCCGTGCCCAACGCATCGTATTGAGCGCCGGAGCCGGCACCGAAGGCTTGCTGCAGGCGCTGGGCCTGGATCAGCCGGCCATGCAAACCCGCCCGCTGCACATGGTCATGGCCAAGGGCCCGAACCTCAAACCTTTGTACGCCCACTGCCTCGGCGGCGGGCCCAAGCCGCGGGT‑AACGGTGACCACCCATCCGGCAGCCGATGGCCAGTGGGTCTGGTACCTCGGTGGGGACCTGGCCGAAGCCGATGGCGTGGC  >  NC_002947/5611557‑5611848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑