Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 422,119 T→A 5.9% E610V (GAG→GTG)  PP_0348 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947422,1190TA 5.9% 211.4 / 2.1 68E610V (GAG→GTG) PP_0348hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/32);  new base A (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GCTGATGAAATCCAGCAAACGCCAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGGTTGTTCGA  >  NC_002947/421974‑422265
                                                                                                                                                 |                                                                                                                                                  
gCTGATGAAATCCAGCAAACGCCAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCgg                                                                                                                                                 >  1:5550/1‑149 (MQ=255)
 cTGATGAAATCCAGCAAACGCCAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCggg                                                                                                                                                >  4:61731/1‑149 (MQ=255)
    atgaAATCCAGCAAACGCCAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGc                                                                                                                                               <  7:230929/147‑1 (MQ=255)
     tgaAATCCAGCAAACGCCAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTg                                                                                                                                            <  7:110473/149‑1 (MQ=255)
                aaaCGCCAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGt                                                                                                                                 <  7:273644/149‑1 (MQ=255)
                      cAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAg                                                                                                                           <  3:87980/149‑1 (MQ=255)
                      cAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCACGCCCGAGGTCaaaga                                                                                                                                    >  4:129493/1‑138 (MQ=255)
                      cAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCACGCCCGAGGTCaaaga                                                                                                                                    <  3:129493/140‑3 (MQ=255)
                                 cATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGt                                                                                                                >  8:260552/1‑149 (MQ=255)
                                  aTGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTc                                                                                                               <  2:5550/149‑1 (MQ=255)
                                  aTGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCCCAGGCGCCCCTTGTc                                                                                                               <  1:70497/149‑1 (MQ=255)
                                   tGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTcc                                                                                                              <  1:186403/149‑1 (MQ=255)
                                   tGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTcc                                                                                                              <  2:265560/149‑1 (MQ=255)
                                   tGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTc                                                                                                               >  6:123812/1‑148 (MQ=255)
                                     aCCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCa                                                                                                             <  3:61731/148‑1 (MQ=255)
                                      ccGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAg                                                                                                            >  5:19903/1‑148 (MQ=255)
                                             cGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCa                                                                                                             >  4:219033/1‑140 (MQ=255)
                                             cGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCa                                                                                                             <  3:219033/140‑1 (MQ=255)
                                                    aCTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGt                                                                                             >  7:137280/1‑149 (MQ=255)
                                                      ttCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTgg                                                                                           >  3:32055/1‑149 (MQ=255)
                                                           tGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGa                                                                                        >  4:278121/1‑147 (MQ=255)
                                                             gtcgtcATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACa                                                                                      <  7:260552/147‑1 (MQ=255)
                                                              tcgtcATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAgg                                                                                    >  5:68537/1‑148 (MQ=255)
                                                                gtcATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGgca                                                                                  <  3:141670/148‑1 (MQ=255)
                                                                   aTGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCACCCGCTGCACCGTGGCGACAGgcagca                                                                               <  1:161500/148‑1 (MQ=255)
                                                                   aTGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCaa                                                                              <  6:68537/149‑1 (MQ=255)
                                                                   aTGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCaa                                                                              <  5:85795/149‑1 (MQ=255)
                                                                            gcgtcggcCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGgcg                                                                     >  2:209500/1‑149 (MQ=255)
                                                                            gcgtcggcCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGgcg                                                                     >  7:64869/1‑149 (MQ=255)
                                                                             cgtcggcCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGgcgc                                                                    <  1:209500/149‑1 (MQ=255)
                                                                             ccgaggcCCGGGCGCTGATCGCCCAGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGgcgc                                                                    >  3:265825/5‑149 (MQ=255)
                                                                                 ggcCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTgg                                                                 <  8:64869/148‑1 (MQ=255)
                                                                                  gcCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGc                                                               >  7:33004/1‑149 (MQ=255)
                                                                                  gcCCGGGCGCTGATCGCCCAGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGc                                                               >  4:284687/1‑149 (MQ=255)
                                                                                    cTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGa                                                                                        <  4:43006/122‑1 (MQ=255)
                                                                                    cTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGa                                                                                        >  3:43006/1‑122 (MQ=255)
                                                                                    cTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCta                                                             >  1:15483/1‑149 (MQ=255)
                                                                                       ggCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCtacg                                                           <  5:123812/148‑1 (MQ=255)
                                                                                          gATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGcccc                                                                                                                    <  1:291436/88‑1 (MQ=255)
                                                                                          gATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGcacc                                                                                                                    >  2:291436/1‑88 (MQ=255)
                                                                                           aTCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCATTGGCAGGCGCTGGGCTAAGTACGc                                                      >  8:115048/1‑149 (MQ=255)
                                                                                           aTCAGCGACCGGGACTCGGCCATGTAGTAGTAGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCtacgtacg                                                       >  4:292950/1‑148 (MQ=255)
                                                                                                  cccGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGgcag                                                                                 <  5:251837/115‑1 (MQ=255)
                                                                                                  cccGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGgcag                                                                                 >  6:251837/1‑115 (MQ=255)
                                                                                                   ccGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACg                                              >  3:184436/1‑149 (MQ=255)
                                                                                                     gggCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTg                                            <  8:33004/149‑1 (MQ=255)
                                                                                                       gCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTggg                                          <  3:278121/149‑1 (MQ=255)
                                                                                                       gCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTggg                                          >  8:158383/1‑149 (MQ=255)
                                                                                                          tCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGt                                                                                             <  7:236636/95‑1 (MQ=255)
                                                                                                          tCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGt                                                                                             >  8:236636/1‑95 (MQ=255)
                                                                                                          tCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAgg                                                                       >  2:151416/1‑117 (MQ=255)
                                                                                                              ccATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTgg                                   <  6:19903/149‑1 (MQ=255)
                                                                                                                       agtCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAAtgt                           <  4:265825/148‑1 (MQ=255)
                                                                                                                              gaaAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAg                     >  7:128044/1‑147 (MQ=255)
                                                                                                                              gaaAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCa                   <  4:32055/149‑1 (MQ=255)
                                                                                                                                      cgtcTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCgg           >  1:145906/1‑149 (MQ=255)
                                                                                                                                       gtcTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCtacgt                                                          >  7:289686/1‑101 (MQ=255)
                                                                                                                                       gtcTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCtacgt                                                          <  8:289686/101‑1 (MQ=255)
                                                                                                                                       gtcTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCggg          >  7:88192/1‑149 (MQ=255)
                                                                                                                                         atttGACCACGCCCGAGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGct                                                     >  8:193546/2‑104 (MQ=255)
                                                                                                                                         atttGACCACGCCCGAGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGCCAGGCGCTGGGCTACGTACGct                                                     <  7:193546/103‑1 (MQ=255)
                                                                                                                                            tGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGgtt        <  2:15483/146‑1 (MQ=255)
                                                                                                                                             gACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGgcg                                 >  2:204582/1‑120 (MQ=255)
                                                                                                                                             gACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGgcg                                 <  1:204582/120‑1 (MQ=255)
                                                                                                                                             gACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGgttgtt     <  4:184436/148‑1 (MQ=255)
                                                                                                                                             gACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCATTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGGTTGTTc    >  8:202935/1‑149 (MQ=255)
                                                                                                                                              aCCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGGTTGTTc    <  7:202935/148‑1 (MQ=255)
                                                                                                                                                cTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGGTTGTTCGa  >  6:42356/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCTGATGAAATCCAGCAAACGCCAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGGTTGTTCGA  >  NC_002947/421974‑422265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑