Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,632,842 T→G 7.9% L791R (CTG→CGG) ‡ putA → proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,632,8420TG 7.9% 197.4 / 6.7 63L791R (CTG→CGG) ‡putAproline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (32/26);  new base G (3/2);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

GTGAAGCCGTGGACTTCCTGCGCTACTACGCGGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCGGGCCGCGGTGAAACTGTCGGTGCCGGCC  >  NC_002947/5632695‑5632988
                                                                                                                                                   |                                                                                                                                                  
gTGAAGCCGTGGACTTCCTGCGCTACTACGCGGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTg                                                                                                                                                   >  4:54141/1‑149 (MQ=255)
   aaGCCGTGGACTTCCTGCGCTACTACGCGGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCt                                                                                                                                                >  8:28149/1‑149 (MQ=255)
       cGTGGACTTCCTGCGCTACTACGCGGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCg                                                                                                                                            >  7:121971/1‑149 (MQ=255)
        gTGGACTTCCTGCGCTACTACGCGGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCAGGGCTGcggc                                                                                                                                           <  2:126713/149‑4 (MQ=255)
         tGGACTTCCTGCGCTACTACGCGGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGc                                                                                                                                          <  4:266771/149‑1 (MQ=255)
             cTTCCTGCGCTACTACGCGGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAAcc                                                                                                                                      <  5:201019/149‑1 (MQ=255)
              ttCCTGCGCTACTACGCGGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAAccc                                                                                                                                     >  4:180332/1‑149 (MQ=255)
              ttCCTGCGCTACTACGCGGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAAccc                                                                                                                                     >  2:205987/1‑149 (MQ=255)
                   gcgcTACTACGCGGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTAc                                                                                                                                >  2:125042/1‑149 (MQ=255)
                    cgcTACTACGCGGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTa                                                                                                                                 <  1:3838/147‑1 (MQ=255)
                     gcTACTACGCGGTGAAGGAACGTAACTACTTCAGCAACTACGCCCACCGCCCCCTGGTTCCTGTGTTGTGAATCAGCCAATGTAACTTCCCGCTGGCTATCTTCACCGGCCAGGTGGCCGAAG‑CCTGGCTGCCGGCAACCCGGTACTgg                                                                                                                             <  2:812/149‑1 (MQ=255)
                            cgcgGTGCAGGCACGTAACGCCTTCAGCAACGCCGCCCACCGCCAACTGGGTCCGGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCACTGGCTGCCGGCAACCCGGTCCTgg                                                                                                                             >  4:174997/1‑143 (MQ=255)
                            cgcgGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGc                                                                                                                       >  4:26141/1‑149 (MQ=255)
                            cgcgGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGc                                                                                                                       <  6:128617/149‑1 (MQ=255)
                            cgcgGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGGCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTTCCGGCAACCCGGTACTgg                                                                                                                             <  3:174997/143‑1 (MQ=255)
                                gTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGc                                                                                                                   >  1:202814/1‑149 (MQ=255)
                                  gCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCg                                                                                                                 <  3:180332/149‑1 (MQ=255)
                                     ggCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGc                                                                                                              <  1:125042/149‑1 (MQ=255)
                                        aCGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCaaa                                                                                                           <  7:124144/149‑1 (MQ=255)
                                        aCGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCaaa                                                                                                           >  5:50357/1‑149 (MQ=255)
                                             aCGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCa                                                                                                      <  7:171602/149‑1 (MQ=255)
                                             aCGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCa                                                                                                      >  8:171602/1‑149 (MQ=255)
                                                        aaCGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATcgccgc                                                                                           >  2:199379/1‑149 (MQ=255)
                                                         acgacgCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCgccgcc                                                                                          >  2:13326/1‑149 (MQ=255)
                                                              gcccaccgcccacTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACAGGCCAAGCCTGCCGACCAAACCCCACAGAACGACGCCCAgcc                                                                                     >  6:164473/1‑147 (MQ=255)
                                                                ccaccgcccacTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCg                                                                                   >  4:106465/1‑149 (MQ=255)
                                                                 caccgcccacTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCCAGCAAACCCCACTGATCGCCGCCCAGGCCGt                                                                                  >  1:131137/1‑149 (MQ=255)
                                                                       ccacTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGGAAACCCCACTGATCGCCGCCCAGGCCGTGCGCct                                                                            >  8:281510/1‑149 (MQ=255)
                                                                        cacTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCctg                                                                           <  7:28149/149‑1 (MQ=255)
                                                                         acTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCctgc                                                                          <  3:26141/149‑1 (MQ=255)
                                                                          cTGGGTCCTGTGGTGTGCATCAGCCAATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCACCCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCctgct                                                                         >  3:17012/1‑149 (MQ=255)
                                                                             ggTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCctgctgct                                                                      <  3:106465/149‑1 (MQ=255)
                                                                                    tggtgTGCATCAGCCCTTGGAACTTCCCGCTGGCGATCTTCTCCGGCCAGGTGGCCGCAGCCATTGCTGCCGGCAACCCGGTACTGGCCATGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGc                                                                <  5:164473/148‑1 (MQ=255)
                                                                                        gtgCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCa                                                           <  7:281510/149‑1 (MQ=255)
                                                                                         tgCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTg                                                              >  3:254962/1‑145 (MQ=255)
                                                                                         tgCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTg                                                              <  4:254962/145‑1 (MQ=255)
                                                                                           cagcAGGCCCTGGAAGTTCCCGCTGGCGATCTTCTCCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATcc                                                        <  4:17012/146‑1 (MQ=255)
                                                                                           cATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCTCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATcc                                                        <  1:205987/149‑1 (MQ=255)
                                                                                           cATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATc                                                         <  1:199379/148‑1 (MQ=255)
                                                                                            aTCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATcc                                                        >  5:266682/1‑148 (MQ=255)
                                                                                                 cccATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAg                                                  <  2:202814/149‑1 (MQ=255)
                                                                                                 cccATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAAACCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAg                                                  >  7:87874/1‑149 (MQ=255)
                                                                                                   cATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGc                                                <  2:131137/149‑1 (MQ=255)
                                                                                                        aaCTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGtgct                                           >  6:78145/1‑149 (MQ=255)
                                                                                                          cTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGtgctg                                          >  8:226141/1‑148 (MQ=255)
                                                                                                                    gCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGcc                               >  5:172756/1‑149 (MQ=255)
                                                                                                                         cTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCGGGcc                          >  4:89068/1‑149 (MQ=255)
                                                                                                                            cACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCgg                             <  6:266682/143‑1 (MQ=255)
                                                                                                                                 gCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCGGGCcgcg                       >  1:156772/1‑144 (MQ=255)
                                                                                                                                 gCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCGGGCcgcg                       <  2:156772/144‑1 (MQ=255)
                                                                                                                                     ggTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAg                                       >  1:129951/1‑124 (MQ=255)
                                                                                                                                     ggTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAg                                       <  2:129951/124‑1 (MQ=255)
                                                                                                                                        gccggCAGCCAGGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAgctgct                                  >  1:285513/5‑126 (MQ=255)
                                                                                                                                         ccggCAGCCAGGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGTCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCGGGCCGCGGTGAAACTGTCgg          >  7:234289/4‑149 (MQ=255)
                                                                                                                                           ggCAGCCAGGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCGGGCCGCGGTGAAACTGTCg           >  3:126139/2‑146 (MQ=255)
                                                                                                                                           ggCAGCCAGGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCGGGCCGCGGTGAAACTGTCg           <  4:126139/145‑1 (MQ=255)
                                                                                                                                           cGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCGGGCCGCGGTGAAACTGTCGGTg        >  4:221043/1‑149 (MQ=255)
                                                                                                                                           cGCAGCCCTGGCTGCCGGCAACAAGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCGGGCCGCGGTGAAACTGTCGGTg        >  7:46818/1‑149 (MQ=255)
                                                                                                                                            gCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCACGCCTGCCGAGCAAACCCCACTGATCGCCGCCCCGGCCGTGCGCCTGCTGCTGGAAGCTGGCATGCCAGAAGGCGTGCTGCAGCTGCTGCCGGGCCGCGGTGAAACTGTCGGTGc       >  5:151057/1‑149 (MQ=255)
                                                                                                                                            gCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCGGGCCGCGGTGAAACTGTCGGTGc       <  3:54141/149‑1 (MQ=255)
                                                                                                                                                cccTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCGGGCCGCGGTGAAACTGt             <  2:244915/139‑1 (MQ=255)
                                                                                                                                                cccTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCGGGCCGCGGTGAAACTGt             >  1:244915/1‑139 (MQ=255)
                                                                                                                                                 ccTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCGGGCCGCGGTGAAACTGTCGGTGCCGGcc  >  4:266102/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTGAAGCCGTGGACTTCCTGCGCTACTACGCGGTGCAGGCACGTAACGACTTCAGCAACGACGCCCACCGCCCACTGGGTCCTGTGGTGTGCATCAGCCCATGGAACTTCCCGCTGGCGATCTTCACCGGCCAGGTGGCCGCAGCCCTGGCTGCCGGCAACCCGGTACTGGCCAAGCCTGCCGAGCAAACCCCACTGATCGCCGCCCAGGCCGTGCGCCTGCTGCTGGAAGCTGGCATCCCAGAAGGCGTGCTGCAGCTGCTGCCGGGCCGCGGTGAAACTGTCGGTGCCGGCC  >  NC_002947/5632695‑5632988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑