Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,634,458 C→A 11.1% intergenic (+34/+349) putA → / ← PP_5718 proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,634,4580CA11.1% 159.2 / 9.9 54intergenic (+34/+349)putA/PP_5718proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase/membrane protein
Reads supporting (aligned to +/- strand):  ref base C (25/23);  new base A (4/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.57e-01

GGGCTGTCCAGCGGGGATCACCAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCTGGGCCGGTCCT  >  NC_002947/5634311‑5634605
                                                                                                                                                   |                                                                                                                                                   
gggCTGTCCAGCGGGGATCACGAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCg                                                                                                                                                    <  8:65601/149‑1 (MQ=255)
 ggCTGTCCAGCGGGGATCACCAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCgg                                                                                                                                                   >  5:128128/1‑149 (MQ=255)
        cAGCGGGGATCACCTGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCGAGGCTCTcc                                                                                                                                             <  3:99361/148‑1 (MQ=255)
        cAGCGGGGATCACCAGATTGCGCTGGAGCTCCTGGTCATCGAGCGCGCGGTGAGAGTGAACACTGAAGCGGAGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCt                                                                                                                                            >  6:313496/1‑149 (MQ=255)
               gATCACCAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTAt                                                                                                                                     <  6:241950/149‑1 (MQ=255)
                                    cgcCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAgg                                                                                                                >  8:269278/1‑149 (MQ=255)
                                       cTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCATGAAGCAGGGGc                                                                                                             >  4:33721/1‑149 (MQ=255)
                                             aTCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGAc                                                                                                        >  2:11258/1‑148 (MQ=255)
                                              tCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGt                                                                                                                                 <  4:261092/122‑1 (MQ=255)
                                              tCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGt                                                                                                                                 >  3:261092/1‑122 (MQ=255)
                                               cGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCGAGGCTCTCcttcttct                                                                                                                                      <  3:142306/116‑3 (MQ=255)
                                               cGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCGAGGCTCTCcttcttct                                                                                                                                      >  4:142306/1‑114 (MQ=255)
                                                    gcgcgGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGt                                                                                                >  3:281394/1‑149 (MQ=255)
                                                        ggTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTc                                                                                            <  6:128128/149‑1 (MQ=255)
                                                          tGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCAc                                                                                          >  4:278634/1‑149 (MQ=255)
                                                             gCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGc                                                                                                             >  1:150171/1‑127 (MQ=255)
                                                             gCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGc                                                                                                             <  2:150171/127‑1 (MQ=255)
                                                                    cacTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCa                                                                                        <  7:288949/141‑1 (MQ=255)
                                                                    cacTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCa                                                                                        >  8:288949/1‑141 (MQ=255)
                                                                     acTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGt                                                                               <  6:269591/149‑1 (MQ=255)
                                                                          aGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGc                                                                          >  5:199904/1‑149 (MQ=255)
                                                                              gcggGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAAc                                                                      <  5:313496/149‑1 (MQ=255)
                                                                                      tAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACtt                                                              >  7:87208/1‑149 (MQ=255)
                                                                                        aCGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTgg                                                            <  1:11258/149‑1 (MQ=255)
                                                                                        aCGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTg                                                             <  3:278634/148‑1 (MQ=255)
                                                                                            cAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGgtgt                                                        >  5:286997/1‑149 (MQ=255)
                                                                                                cTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGtc                                                       <  4:10568/146‑1 (MQ=255)
                                                                                                cTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGtc                                                       >  3:10568/1‑146 (MQ=255)
                                                                                                   atgaCCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGtcttct                                                   <  1:190286/147‑1 (MQ=255)
                                                                                                   atgaCCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGtcttct                                                   <  8:237745/147‑1 (MQ=255)
                                                                                                   atgaCCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGtcttca                                                   <  6:199904/147‑2 (MQ=255)
                                                                                                    tgaCCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCtgtt                                                <  6:94026/149‑1 (MQ=255)
                                                                                                     gaCCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTg                                                                                               <  5:265861/101‑1 (MQ=255)
                                                                                                     gaCCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTg                                                                                               >  6:265861/1‑101 (MQ=255)
                                                                                                         aTTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTAc                                           <  8:87208/149‑1 (MQ=255)
                                                                                                               tAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGt                                                                   <  1:195921/119‑1 (MQ=255)
                                                                                                               tAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGt                                                                   >  2:195921/1‑119 (MQ=255)
                                                                                                                   ggCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATg                                                                                                                            >  1:141119/1‑58 (MQ=255)
                                                                                                                   ggCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATg                                                                                                                            <  2:141119/58‑1 (MQ=255)
                                                                                                                          ggTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGaa                           >  8:117662/1‑148 (MQ=255)
                                                                                                                                  aagaagaagGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGc                  >  3:80832/1‑149 (MQ=255)
                                                                                                                                  aagaagaagGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGc                  >  4:31380/1‑149 (MQ=255)
                                                                                                                                    gaagaagGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTg                >  2:284059/1‑149 (MQ=255)
                                                                                                                                      aaaagGAGAGCCGAGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGcc              >  2:202327/3‑149 (MQ=255)
                                                                                                                                        aagGAGAGCCGAGGCTCTCCTTTTTTATGCGCGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCTTGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCTg            >  6:271900/1‑149 (MQ=255)
                                                                                                                                         agGAGAGCCGAGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCTgg           >  3:222905/1‑149 (MQ=255)
                                                                                                                                          ggAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATccc                                 >  4:252459/1‑126 (MQ=255)
                                                                                                                                          ggAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCTggg          <  1:284059/149‑1 (MQ=255)
                                                                                                                                           gagaGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCTGGGc         <  4:80832/149‑1 (MQ=255)
                                                                                                                                             gagCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCTGGGCCg       >  1:21350/1‑149 (MQ=255)
                                                                                                                                                ccTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCTGGGCCGGTc    <  4:281394/149‑1 (MQ=255)
                                                                                                                                                 cTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTg                                                                                 >  2:156724/1‑71 (MQ=255)
                                                                                                                                                 cTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTg                                                                                 <  1:156724/71‑1 (MQ=255)
                                                                                                                                                  tCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAATCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCTGGGCCGGTCCt  >  7:40064/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGGCTGTCCAGCGGGGATCACCAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCTGGGCCGGTCCT  >  NC_002947/5634311‑5634605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑