Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,650,675 T→A 11.6% H173L (CAT→CTT) ‡ PP_4959 ← diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,650,6750TA11.6% 159.3 / 10.5 60H173L (CAT→CTT) ‡PP_4959diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (30/23);  new base A (5/2);  total (35/25)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

TAGGCCTTGTAGAAGTCCGACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGA  >  NC_002947/5650534‑5650822
                                                                                                                                             |                                                                                                                                                   
tAGGCCTTGTAGAAGTCCGACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATc                                                                                                                                              <  8:139218/149‑1 (MQ=255)
   gCCTTGTAGAAGTCCGACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAac                                                                                                                                           <  3:280846/149‑1 (MQ=255)
    ccTTGTAGAAGTCCGACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATc                                                                                                                                              >  2:283059/1‑145 (MQ=255)
    ccTTGTAGAAGTCCGACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATc                                                                                                                                              <  1:283059/145‑1 (MQ=255)
            aaGTCCGACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCGCCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCCATGGCATCAtccgtt                                                                                                                                       <  2:81027/144‑4 (MQ=255)
            aaGTCCGACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCGCCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCCATGGCATCAtccgtt                                                                                                                                       >  1:81027/1‑141 (MQ=255)
            aaGTCCGACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGAGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTTGCAAAACGGATGATGCCATGGGCATCAACCACCAGAAt                                                                                                                                  <  8:241072/149‑1 (MQ=255)
              gTCCGACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAACCGGGAAATGGCCGGATTGGCAAAACGGATTATGCCATGGGCATCAACCACCAGAATGc                                                                                                                                >  8:68695/1‑149 (MQ=255)
              gTCCGACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGc                                                                                                                                <  2:220458/149‑1 (MQ=255)
                 cGACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCCATGGCATCAtccg                                                                                                                                         >  7:150744/1‑136 (MQ=255)
                 cGACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCCATGGCATCAtccg                                                                                                                                         <  8:150744/137‑2 (MQ=255)
                  gACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTc                                                                                                                            <  1:235619/149‑1 (MQ=255)
                  gACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTc                                                                                                                            <  3:20271/149‑1 (MQ=255)
                       gcgcCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTcggcg                                                                                                                        <  1:205441/148‑1 (MQ=255)
                        cgcCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAaccacc                                                                                                                                       <  6:205990/132‑1 (MQ=255)
                        cgcCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAaccacc                                                                                                                                       >  5:205990/1‑132 (MQ=255)
                        cgcCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCggcggc                                                                                                                      <  5:267396/149‑1 (MQ=255)
                         gcCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCa                                                                                                                     >  7:112723/1‑149 (MQ=255)
                                     tACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATg                                                                                                         >  6:34366/1‑149 (MQ=255)
                                         ggTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAggtcttggc                                                                                                     >  1:122193/1‑142 (MQ=255)
                                                   acaACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGc                                                                                           >  2:288646/1‑149 (MQ=255)
                                                    caACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGcc                                                                                          >  4:78730/1‑149 (MQ=255)
                                                         tCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGc                                                                                     <  8:156888/149‑1 (MQ=255)
                                                            aGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAAt                                                                                                                                  >  8:201384/1‑101 (MQ=255)
                                                            aGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAAt                                                                                                                                  <  7:201384/101‑1 (MQ=255)
                                                                     gTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTc                                                                         <  5:34366/149‑1 (MQ=255)
                                                                          cTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGa                                                                     >  8:69884/1‑148 (MQ=255)
                                                                            gCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCa                                                                        >  4:2381/1‑143 (MQ=255)
                                                                            gCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCa                                                                        <  3:2381/143‑1 (MQ=255)
                                                                               ggTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAgc                                                               >  8:241083/1‑149 (MQ=255)
                                                                                 tgtTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAgctg                                                             >  6:240072/1‑149 (MQ=255)
                                                                                       aCCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTgcag                                                       >  4:131289/1‑149 (MQ=255)
                                                                                         cGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCtgctgc                                                         <  8:222450/145‑1 (MQ=255)
                                                                                         cGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCtgctgc                                                         >  7:222450/1‑145 (MQ=255)
                                                                                          ggTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGtctc                                                                               <  1:68623/122‑1 (MQ=255)
                                                                                          ggTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGtctc                                                                               >  2:68623/1‑122 (MQ=255)
                                                                                                gccAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCg                                              >  2:303795/1‑149 (MQ=255)
                                                                                                 ccAGCAAACGGGAAATGGCAGTATTTTCAAAACGGATGATTAAATGGGCATCAACCACCAGAATGCCTTCGGCGGCATATTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCATCTCACGACTCAGCTGCTGCAGCATGCTCCgt                                             >  4:185337/1‑148 (MQ=255)
                                                                                                  cAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGt                                            >  4:36004/1‑149 (MQ=255)
                                                                                                   aGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGtt                                           <  3:78730/149‑1 (MQ=255)
                                                                                                      aaaCGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTgcg                                        >  8:150396/1‑149 (MQ=255)
                                                                                                                 ggCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTc                               >  1:127558/1‑147 (MQ=255)
                                                                                                                 ggCCGGATTGGCAAAACGGATGATGCCATGCGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTc                               >  1:25964/1‑147 (MQ=255)
                                                                                                                  gCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGag                            <  1:303795/149‑1 (MQ=255)
                                                                                                                    cGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTc                                                                  >  7:204653/1‑109 (MQ=255)
                                                                                                                    cGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTc                                                                  <  8:204653/109‑1 (MQ=255)
                                                                                                                     ggATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGagcag                         <  8:112723/149‑1 (MQ=255)
                                                                                                                     ggATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGAGACGGTTGCGCTGCTGGTCGagcag                         >  1:271745/1‑149 (MQ=255)
                                                                                                                           gCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTg                   <  2:25964/149‑1 (MQ=255)
                                                                                                                            cAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTg                   >  1:165199/1‑148 (MQ=255)
                                                                                                                             aaaaCGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGAATTGAAGGCCCTGGCCGTCTCCAGCTAACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGa                 >  8:193462/1‑149 (MQ=255)
                                                                                                                              aaaCGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGAc                >  5:39456/1‑149 (MQ=255)
                                                                                                                                   gttgatgCCCATGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTgg           >  8:93688/3‑149 (MQ=255)
                                                                                                                                   gttgatgCCCATGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTgg           >  6:172380/3‑149 (MQ=255)
                                                                                                                                   gatgatGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGAcc               <  7:62421/145‑1 (MQ=255)
                                                                                                                                   gatgatGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGAcc               >  8:62421/1‑145 (MQ=255)
                                                                                                                                    ttgatgCCCATGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTggg          >  5:147545/2‑149 (MQ=255)
                                                                                                                                           cATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCtgctgc                                                         >  4:166963/1‑95 (MQ=255)
                                                                                                                                           cATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCtgctgc                                                         <  3:166963/95‑1 (MQ=255)
                                                                                                                                            aTGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGa  >  1:65470/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
TAGGCCTTGTAGAAGTCCGACTCGCGCCACAGGCTGGTACTGGTCAGTTGCACAACGTCGAGCAATTGGGTGCCCTGCAGGTGTTGCACCGGTGCCGCCAGCAAACGGGAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGA  >  NC_002947/5650534‑5650822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑