Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 422,123 G→C 7.0% P609A (CCC→GCC)  PP_0348 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947422,1230GC 7.0% 215.0 / 3.2 67P609A (CCC→GCC) PP_0348hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (30/32);  new base C (3/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGATGAAATCCAGCAAACGCCAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGGTTGTTCGAAAACGG  >  NC_002947/421975‑422271
                                                                                                                                                    |                                                                                                                                                    
cTGATGAAATCCAGCAAACGCCAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCggg                                                                                                                                                      >  4:61731/1‑149 (MQ=255)
   atgaAATCCAGCAAACGCCAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGc                                                                                                                                                     <  7:230929/147‑1 (MQ=255)
    tgaAATCCAGCAAACGCCAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTg                                                                                                                                                  <  7:110473/149‑1 (MQ=255)
               aaaCGCCAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGt                                                                                                                                       <  7:273644/149‑1 (MQ=255)
                     cAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAg                                                                                                                                 <  3:87980/149‑1 (MQ=255)
                     cAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCACGCCCGAGGTCaaaga                                                                                                                                          >  4:129493/1‑138 (MQ=255)
                     cAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCACGCCCGAGGTCaaaga                                                                                                                                          <  3:129493/140‑3 (MQ=255)
                                cATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGt                                                                                                                      >  8:260552/1‑149 (MQ=255)
                                 aTGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTc                                                                                                                     <  2:5550/149‑1 (MQ=255)
                                 aTGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCCCAGGCGCCCCTTGTc                                                                                                                     <  1:70497/149‑1 (MQ=255)
                                  tGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTcc                                                                                                                    <  1:186403/149‑1 (MQ=255)
                                  tGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTcc                                                                                                                    <  2:265560/149‑1 (MQ=255)
                                  tGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTc                                                                                                                     >  6:123812/1‑148 (MQ=255)
                                    aCCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCa                                                                                                                   <  3:61731/148‑1 (MQ=255)
                                     ccGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAg                                                                                                                  >  5:19903/1‑148 (MQ=255)
                                            cGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCa                                                                                                                   >  4:219033/1‑140 (MQ=255)
                                            cGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCa                                                                                                                   <  3:219033/140‑1 (MQ=255)
                                                   aCTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGt                                                                                                   >  7:137280/1‑149 (MQ=255)
                                                     ttCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTgg                                                                                                 >  3:32055/1‑149 (MQ=255)
                                                          tGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGa                                                                                              >  4:278121/1‑147 (MQ=255)
                                                            gtcgtcATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACa                                                                                            <  7:260552/147‑1 (MQ=255)
                                                             tcgtcATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAgg                                                                                          >  5:68537/1‑148 (MQ=255)
                                                               gtcATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGgca                                                                                        <  3:141670/148‑1 (MQ=255)
                                                                  aTGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCACCCGCTGCACCGTGGCGACAGgcagca                                                                                     <  1:161500/148‑1 (MQ=255)
                                                                  aTGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCaa                                                                                    <  6:68537/149‑1 (MQ=255)
                                                                  aTGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCaa                                                                                    <  5:85795/149‑1 (MQ=255)
                                                                           gcgtcggcCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGgcg                                                                           >  2:209500/1‑149 (MQ=255)
                                                                           gcgtcggcCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGgcg                                                                           >  7:64869/1‑149 (MQ=255)
                                                                            cgtcggcCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGgcgc                                                                          <  1:209500/149‑1 (MQ=255)
                                                                            ccgaggcCCGGGCGCTGATCGCCCAGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGgcgc                                                                          >  3:265825/5‑149 (MQ=255)
                                                                                ggcCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTgg                                                                       <  8:64869/148‑1 (MQ=255)
                                                                                 gcCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGc                                                                     >  7:33004/1‑149 (MQ=255)
                                                                                 gcCCGGGCGCTGATCGCCCAGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGc                                                                     >  4:284687/1‑149 (MQ=255)
                                                                                   cTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGa                                                                                              >  3:43006/1‑122 (MQ=255)
                                                                                   cTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGa                                                                                              <  4:43006/122‑1 (MQ=255)
                                                                                   cTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCta                                                                   >  1:15483/1‑149 (MQ=255)
                                                                                      ggCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCtacg                                                                 <  5:123812/148‑1 (MQ=255)
                                                                                         gATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGcccc                                                                                                                          <  1:291436/88‑1 (MQ=255)
                                                                                         gATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGcacc                                                                                                                          >  2:291436/1‑88 (MQ=255)
                                                                                          aTCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCATTGGCAGGCGCTGGGCTAAGTACGc                                                            >  8:115048/1‑149 (MQ=255)
                                                                                          aTCAGCGACCGGGACTCGGCCATGTAGTAGTAGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCtacgtacg                                                             >  4:292950/1‑148 (MQ=255)
                                                                                                 cccGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGgcag                                                                                       <  5:251837/115‑1 (MQ=255)
                                                                                                 cccGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGgcag                                                                                       >  6:251837/1‑115 (MQ=255)
                                                                                                  ccGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACg                                                    >  3:184436/1‑149 (MQ=255)
                                                                                                    gggCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTg                                                  <  8:33004/149‑1 (MQ=255)
                                                                                                      gCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTggg                                                >  8:158383/1‑149 (MQ=255)
                                                                                                      gCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTggg                                                <  3:278121/149‑1 (MQ=255)
                                                                                                         tCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGt                                                                                                   >  8:236636/1‑95 (MQ=255)
                                                                                                         tCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGt                                                                                                   <  7:236636/95‑1 (MQ=255)
                                                                                                         tCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAgg                                                                             >  2:151416/1‑117 (MQ=255)
                                                                                                             ccATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTgg                                         <  6:19903/149‑1 (MQ=255)
                                                                                                                      agtCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAAtgt                                 <  4:265825/148‑1 (MQ=255)
                                                                                                                             gaaAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAg                           >  7:128044/1‑147 (MQ=255)
                                                                                                                             gaaAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCa                         <  4:32055/149‑1 (MQ=255)
                                                                                                                                     cgtcTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCgg                 >  1:145906/1‑149 (MQ=255)
                                                                                                                                      gtcTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCtacgt                                                                >  7:289686/1‑101 (MQ=255)
                                                                                                                                      gtcTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCtacgt                                                                <  8:289686/101‑1 (MQ=255)
                                                                                                                                      gtcTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCggg                >  7:88192/1‑149 (MQ=255)
                                                                                                                                        atttGACCACGCCCGAGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGct                                                           >  8:193546/2‑104 (MQ=255)
                                                                                                                                        atttGACCACGCCCGAGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGCCAGGCGCTGGGCTACGTACGct                                                           <  7:193546/103‑1 (MQ=255)
                                                                                                                                           tGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGgtt              <  2:15483/146‑1 (MQ=255)
                                                                                                                                            gACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGgcg                                       >  2:204582/1‑120 (MQ=255)
                                                                                                                                            gACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGgcg                                       <  1:204582/120‑1 (MQ=255)
                                                                                                                                            gACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGgttgtt           <  4:184436/148‑1 (MQ=255)
                                                                                                                                            gACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCATTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGGTTGTTc          >  8:202935/1‑149 (MQ=255)
                                                                                                                                             aCCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGGTTGTTc          <  7:202935/148‑1 (MQ=255)
                                                                                                                                               cTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGGTTGTTCGa        >  6:42356/1‑148 (MQ=255)
                                                                                                                                                 cGGCCGTGGTCAAATGCGTCCTCAGCCGCACCTTCTCCAGCAACCGCTGCAACCTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACCTACGCTCTCAACGTCCCCTGTTTGCGCAATCTCCGAAGCAGTTCGCGGGTTGTTCGaaaa     >  4:109589/1‑149 (MQ=255)
                                                                                                                                                    gCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGGTTGTTCGAAAACgg  <  8:88192/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGATGAAATCCAGCAAACGCCAGCTCAGCGCCATGACCGACTCCGCATTCACTTCCCTGGTCGTCATGCTGTCGGCGTCGGCCTGGGCGATCAGCGCCCGGGCCTCGGCCATGTAGTAGTCGAAGAAAAGGTCGTCTTTGACCTCGGGCGTGGTCAAATGCGTGCTCAGGCGCCCCTTGTCCAGCAACCGCTGCACCGTGGCGACAGGCAGCAATGGCAGGCGCTGGGCTACGTACGCTCTCAACGTGGGCTGTTGGCGCAATGTGCGAAGCAGTTCGCGGGTTGTTCGAAAACGG  >  NC_002947/421975‑422271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑