Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 432,012 A→G 8.7% F101S (TTC→TCC)  PP_0354 ← CBS domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947432,0120AG 8.7% 126.2 / 3.1 46F101S (TTC→TCC) PP_0354CBS domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (23/19);  new base G (2/2);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

ACACCGCGCAGGGCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCG  >  NC_002947/431865‑432158
                                                                                                                                                    |                                                                                                                                                  
acacCGCGCATGGCAAAATCCAGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGaa                                                                                                                                                   <  8:99736/149‑1 (MQ=255)
     gcgcAGGGCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCAc                                                                                                                                               <  3:188113/148‑1 (MQ=255)
     gcgcAGGGCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCAc                                                                                                                                               >  4:188113/1‑148 (MQ=255)
          gggCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCg                                                                                                                                         >  4:21751/1‑149 (MQ=255)
              aaacacccGAAACACATCCGACATCGAGAACACGCGGAAGAATGCGGCCTTGTTCACTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCACGGCTGCCAGCGGGGAGTGCTTCCCGCTgg                                                                                                                                     <  6:69291/144‑1 (MQ=255)
                aaTCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGGAGTGCTTCCCGCTggca                                                                                                                                   <  1:5422/149‑3 (MQ=255)
                aaTCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTg                                                                                                                                   <  2:164187/149‑1 (MQ=255)
                      gAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGt                                                                                                                                    >  6:227961/1‑142 (MQ=255)
                      gAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGt                                                                                                                                    <  5:227961/142‑1 (MQ=255)
                             aTCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTgg                                                                                                                      >  4:230326/1‑149 (MQ=255)
                              tCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTGACCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTgg                                                                                                                      >  1:87723/1‑149 (MQ=255)
                                aGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAAc                                                                                                                          <  2:213271/142‑1 (MQ=255)
                                aGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAAc                                                                                                                          >  1:213271/1‑142 (MQ=255)
                                aGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGttt                                                                                                                   <  7:95341/149‑1 (MQ=255)
                                 gACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTc                                                                                                                  >  2:150340/1‑149 (MQ=255)
                                     tCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGaca                                                                                                              >  7:252868/1‑149 (MQ=255)
                                      cGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGCTCTCGACCGTGGTTTCGacac                                                                                                             >  8:56058/1‑149 (MQ=255)
                                        agaACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACAccc                                                                                                           <  3:21751/149‑1 (MQ=255)
                                               gcgGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTgg                                                                                                    >  2:12653/1‑149 (MQ=255)
                                                       aTGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGc                                                                                            <  5:297898/149‑1 (MQ=255)
                                                           ggCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGgc                                                                                        >  7:21332/1‑149 (MQ=255)
                                                                 gTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTc                                                                                    <  3:230326/147‑1 (MQ=255)
                                                                      gTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAAcc                                                                             <  4:67850/149‑1 (MQ=255)
                                                                                gAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGc                                                                                                   <  6:228892/117‑1 (MQ=255)
                                                                                gAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGc                                                                                                   >  5:228892/1‑117 (MQ=255)
                                                                                gAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCt                                                                   >  4:88657/1‑149 (MQ=255)
                                                                                gAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCt                                                                   <  8:21332/149‑1 (MQ=255)
                                                                                 aaGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGcc                                                                     <  1:296897/146‑1 (MQ=255)
                                                                                 aaGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGcc                                                                     >  2:296897/1‑146 (MQ=255)
                                                                                      gtCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGa                                                             >  5:30628/1‑149 (MQ=255)
                                                                                       tCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGAc                                                            >  1:286584/1‑149 (MQ=255)
                                                                                            ccccGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATg                                                        >  5:20503/1‑149 (MQ=255)
                                                                                              ccGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTa                                                      <  2:286584/149‑1 (MQ=255)
                                                                                                 gCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAg                                                     >  3:251516/1‑147 (MQ=255)
                                                                                                     ggTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGc                                               <  8:252868/149‑1 (MQ=255)
                                                                                                          tCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGAc                                          >  5:23073/1‑149 (MQ=255)
                                                                                                            ggTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGAcc                                         <  6:20503/148‑1 (MQ=255)
                                                                                                              tGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGc                                      <  7:56058/149‑1 (MQ=255)
                                                                                                               gCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGcc                                     >  2:16016/1‑149 (MQ=255)
                                                                                                                cGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGccc                                    <  3:88657/149‑1 (MQ=255)
                                                                                                                cGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGccc                                    <  6:30628/149‑1 (MQ=255)
                                                                                                                      gCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTACTTGACGATGTAGAAGTGCTCGACCGGCCCGTCgg                               <  6:41031/148‑1 (MQ=255)
                                                                                                                      gCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTACTTGACGATGTAGAAGTGCTCGACCGGCCCGTCgg                               >  5:41031/1‑148 (MQ=255)
                                                                                                                                ccAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGAc                     >  8:22965/1‑148 (MQ=255)
                                                                                                                                      agatcaccAGCGGGGAGTGCTTCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCtt              >  6:33843/7‑149 (MQ=255)
                                                                                                                                           accAGCGGGGAGTGCTTCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCAcc         >  4:167421/2‑149 (MQ=255)
                                                                                                                                                   gAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCg  >  3:161138/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACACCGCGCAGGGCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCG  >  NC_002947/431865‑432158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑